We narrowed to 4,353 results for: erf

1. pLX-TRE-dCas9-KRAB-MeCP2-BSD

   Plasmid

   #140690

   Purpose

   Inducible knockdown of gene expression in human cells for pooled scRNA-seq experiments

   Depositor

   Andrea Califano

   Article

   Tan et al bioRxiv 2021.06.28.449297

   Insert

   Cas9m4-KRAB-MeCP2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCRISPRi_Mxi1_yl

   Plasmid

   #91248

   Purpose

   CRISPR-dCas9-Mxi1 vector for Yarrowia lipolytica, expressing dCas9-Mxi1 and AvrII site for sgRNA insertion

   Depositor

   Ian Wheeldon

   Article

   Schwartz et al Biotechnol Bioeng. 2017 Aug 19. doi: 10.

   Inserts

   Codon optimized dCas9-Mxi1
   sgRNA expression cassette
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   SCR1'-tRNA and UAS1B8-TEF(136)

   Available Since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCRISPathBrick

   Plasmid

   #65006

   Purpose

   E. coli vector for expression of S. pyogenes dCas9, tracrRNA, and nontargeting CRISPR array with BsaI site for inserting user-defined spacer-repeat bricks

   Depositor

   Mattheos Koffas

   Article

   Cress et al ACS Synth Biol. 2015 Mar 30.

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Constitutive native promoters

   Available Since

   May 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV CMV-dSaCas9-KRAB-bGHpA

   Plasmid

   #106219

   Purpose

   AAV expressing deactivated S. aureus Cas9 fused to a KRAB repressor

   Depositor

   Charles Gersbach

   Article

   Thakore et al Nat Commun. 2018 Apr 26;9(1):1674. doi:

   Insert

   dead S. aureus Cas9 KRAB
   

   Use

   AAV and CRISPR

   Tags

   HA-NLS

   Expression

   Mammalian

   Mutation

   D10A and N580A

   Promoter

   CMV

   Available Since

   May 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. CAPTURE2_pLVX-EF1a-dCas9-CBio-IRES-zsGreen1

   Plasmid

   #138418

   Purpose

   CAPTURE2.0 vector containing dCas9-CBio, IRES and zsGreen1

   Depositor

   Jian Xu

   Article

   Liu et al Genome Biol. 2020 Mar 5;21(1):59. doi: 1

   Insert

   dCas9
   

   Use

   Lentiviral

   Tags

   BioTAP-tag

   Expression

   Mutation

   Promoter

   EF1a

   Available Since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pOmniBac zz TEV YBBR TRF2

   Plasmid

   #185443

   Purpose

   Expresses human TRF2 with a zz affinity tag, YBBR labeling site, and TEV cleavage site in insect cells

   Depositor

   Ahmet Yildiz

   Article

   Jack et al Dev Cell. 2022 Jan 24;57(2):277-290.e9.

   Insert

   TRF2 (TERF2 Human)
   

   Use

   Tags

   YBBR and ZZ

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCB-1

   Plasmid

   #210387

   Purpose

   All-in-one cumate-based inducible CRISPRi plasmid for use in Streptomyces

   Depositor

   Gilles van Wezel

   Article

   Bai et al ACS Synth Biol. 2023 Oct 6. doi: 10.1021

   Inserts

   dCas9
   CymR
   sgRNA cassette
   

   Use

   CRISPR; Integrative vector for use in streptomyces

   Tags

   Expression

   Mutation

   BsaI-flanked RFP cassette inserted in place of sp…

   Promoter

   SP11 and SP30

   Available Since

   Jan. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-pMecp2-dSaCas9-KRAB-pU6-sgRNA

   Plasmid

   #158988

   Purpose

   Vector E encodes pAAV-pMecp2-dSaCas9-KRAB-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of CRISPR interference in neurons

   Depositor

   Chung Tin

   Article

   Lau et al Mol Ther Nucleic Acids. 2019 Jun 7;16:63

   Insert

   dSaCas9-KRAB
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pMecp2

   Available Since

   Aug. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pdCas9-humanized

   Plasmid

   #44246

   Purpose

   Expression of a catalytically inactive, human codon-optimized Cas9 under the control of Murine Stem Cell retroVirus LTR promoter for mammalian gene knockdown

   Depositor

   Stanley Qi

   Article

   Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

   Insert

   dead Cas9 with 3X NLS
   

   Use

   CRISPR and Retroviral; Vector

   Tags

   3x NLS

   Expression

   Mammalian

   Mutation

   D10A H840A (catalytically inactive)

   Promoter

   MSCV LTR promoter

   Available Since

   April 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCB-2

    Plasmid

    #210388

    Purpose

    All-in-one cumate-based inducible CRISPRi plasmid for use in Streptomyces

    Depositor

    Gilles van Wezel

    Article

    Bai et al ACS Synth Biol. 2023 Oct 6. doi: 10.1021

    Inserts

    dCas9
    CymR
    sgRNA cassette
    

    Use

    CRISPR; Integrative vector for use in streptomyces

    Tags

    Expression

    Mutation

    BsaI-flanked RFP cassette inserted in place of sp…

    Promoter

    SP11 and SP30

    Available Since

    Jan. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. CAHS2 (pBS0249)

    Plasmid

    #185139

    Purpose

    For the mammalian expression of the tardigrade protein CAHS2. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    CAHS2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pYPQ223 (dAsCpf1-SRDX)

    Plasmid

    #86209

    Purpose

    Deactivated AsCpf1-SRDX repressor fusion; transcriptional repressor dCpf1 Gateway entry plasmid

    Depositor

    Yiping Qi

    Article

    Tang et al Nat Plants. 2017 Feb 17;3:17018. doi: 10

    Insert

    dAsCpf1
    

    Use

    Gateway compatible cpf1 vectors

    Tags

    NLS and SRDX repressor and NLS

    Expression

    Plant

    Mutation

    D908A

    Promoter

    Available Since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTDH3-dCas9-Mxi1

    Plasmid

    #46921

    Purpose

    Yeast CEN/ARS vector (Leu2) that contains dCas9 fused to NLS and Mxi1 domain controlled by TDH3 promoter

    Depositor

    Stanley Qi, Jonathan Weissman

    Article

    Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

    Insert

    dCas9-Mxi1
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TDH3

    Available Since

    Oct. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJMP1339

    Plasmid

    #119271

    Purpose

    for cloning new sgRNAs

    Depositor

    Carol Gross, Oren Rosenberg, Jason Peters

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    dcas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Promoter

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJMP1337

    Plasmid

    #119270

    Purpose

    for cloning new sgRNAs

    Depositor

    Carol Gross, Oren Rosenberg, Jason Peters

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    dcas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Promoter

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLX303-ABI1-dCas9

    Plasmid

    #154474

    Purpose

    Expresses ABI1 fused to the N terminus of dCas9

    Depositor

    Mikko Taipale

    Article

    Alerasool et al Nat Methods. 2020 Nov;17(11):1093-1096.

    Insert

    ABI1 (ABI1 Mustard Weed)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Includes ABI1 aa 126-423, D143A

    Promoter

    CMV

    Available Since

    Oct. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. lentiCRISPRi(v1)-Blast

    Plasmid

    #170067

    Purpose

    Expresses dCas9 repressor KRAB-dCas9 and blasticidin resistance

    Depositor

    Neville Sanjana

    Article

    Morris et al Science. 2023 May 19;380(6646):eadh7699.

    Insert

    KRAB-dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    2A and 2xHA tag

    Expression

    Mammalian

    Mutation

    Promoter

    EFS-NS

    Available Since

    Oct. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCRISPRi_Mxi1_yl_NHEJ

    Plasmid

    #91249

    Purpose

    CRISPRi vector for Yarrowia lipolytica, repressing KU70 and KU80 for enhanced HR

    Depositor

    Ian Wheeldon

    Article

    Schwartz et al Biotechnol Bioeng. 2017 Aug 19. doi: 10.

    Inserts

    Codon optimized dCas9-Mxi1
    KU70 sgRNA expression cassette
    KU80a sgRNA expression cassette
    KU80b sgRNA expression cassette
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SCR1'-tRNA and UAS1B8-TEF(136)

    Available Since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. NAX_CAGGS_CAS9_m4_KRAB-MeCP2_KanR neo

    Plasmid

    #167901

    Purpose

    PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9-KRAB-MeCP2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, D839A, H840A, N863A

    Promoter

    Available Since

    Sept. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLV hUbC-dSaCas9-KRAB-T2A-PuroR

    Plasmid

    #106249

    Purpose

    Lentiviral vector with puro selection for expression of S. aureus dCas9-KRAB

    Depositor

    Charles Gersbach

    Article

    Thakore et al Nat Commun. 2018 Apr 26;9(1):1674. doi:

    Insert

    dead S. aureus Cas9 KRAB T2A PuroR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA-NLS and NLS-KRAB

    Expression

    Mammalian

    Mutation

    D10A and N580A

    Promoter

    hUbC

    Available Since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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