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We narrowed to 6,447 results for: nls

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Showing: 3681 - 3700 of 6447 results

  1. ACBE-Q

    Plasmid
    #204608
    Purpose
    Mammalian expression of high-accuracy adenine-to-cytosine base editor ACBE-Q
    Depositor
    Dali Li
    Article
    Chen et al Nat Biotechnol. 2023 Jun 15. doi: 10.103
    Insert
    bpNLS-N terminal of nSpCas9-linker-mAAG (R165E/Y179F)-TadA-8e (N108Q)-linker-C terminal of nSpCas9-bpNLS-P2A-GFP
    Use
    CRISPR
    Tags
    6*His
    Expression
    Mammalian
    Mutation
    TadA-8e (N108Q); mAAG (R165E/Y179F); S. pyogenes …
    Available Since
    Aug. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    204608-plasmid-map-sequence-id-404717

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  2. AAV-pCbh-SpRY[C-term]-gRNAentry (CA20)

    Plasmid
    #197511
    Purpose
    Cbh promoter expression plasmid for C-terminal intein-split AAV construct with C-term of SpRY and BsmBI entry cassette to clone SpCas9 gRNA spacer
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    AAV-[ITR]-pCbh-BPNLS-NpuC-SpRY[C-term]-BPNLS-gRNA[BsmBI]-pU6-[ITR]
    Use
    AAV and CRISPR
    Tags
    BPNLS and BPNLS-NpuC(intein)
    Mutation
    C-terminal mutations of SpRY(L1111R/D1135L/S1136W…
    Promoter
    Cbh
    Available Since
    March 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    197511-plasmid-map-sequence-id-386647

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  8. Click editor (CE1) - pCMV-T7-PCV2-nCas9-EcKlenow (EMK488)

    Plasmid
    #208942
    Purpose
    The CE1 construct, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    PCV2-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)
    Promoter
    CMV and T7
    Available Since
    Nov. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    208942-plasmid-map-sequence-id-415667

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  9. AAV-pCbh-ABE8e-nSpCas9(D10A/A61R)[N-term] (CA19)

    Plasmid
    #197510
    Purpose
    Cbh promoter expression plasmid for N-terminal intein-split AAV construct with N-term of ABE8e-nSpCas9(D10A/A61R) (to be used with SpRY C-term constructs)
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    AAV-[ITR]-pCBh-BPNLS-TadA8e-nSpCas9(D10A/A61R)[N-term]-NpuN-BPNLS-[ITR]
    Use
    AAV and CRISPR
    Tags
    BPNLS and NpuN(intein)-BPNLS
    Mutation
    ABE8e mutations in TadA and nSpRY(D10A/A61R)
    Promoter
    Cbh
    Available Since
    March 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    197510-plasmid-map-sequence-id-386692

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  10. pCMV-T7-ABE8e-nSpG-P2A-EGFP (KAC984)

    Plasmid
    #185911
    Purpose
    pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    human codon optimized ABE8e-nSpG-P2A-EGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpG=D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T133…
    Promoter
    CMV and T7
    Available Since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    185911-plasmid-map-sequence-id-367364

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  12. P2880

    Plasmid
    #248830
    Purpose
    All repressors for the WTC848 system.
    Depositor
    Joerg Stelling
    Insert
    LacI(6m), LacI(5m)
    Tags
    SV40NLS on LacI(6m)
    Expression
    Yeast
    Mutation
    LacI(6m): T167A, W220F, M254L, V119I, Q60G, M242L…
    Promoter
    pRnr2 (LacI(6m), P4Lacn.2 (LacI(5m))
    Available Since
    Jan. 15, 2026
    Availability
    Academic Institutions and Nonprofits only
    248830-plasmid-map-sequence-id-497160

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  13. pLenti EF1a VP64-NtcA-2xNLS_SV40

    Plasmid
    #254308
    Purpose
    Constitutive expression of VP64-NtcA-2xNLS_SV40 for use in the functional aKG-ON biosensor. Requires pLenti Neo glnA PCC 6803 reporter gene plasmid.
    Depositor
    Samuel McBrayer
    Article
    Sternisha et al Science. 2026 Jul 16;393(6808):eadx8675.
    Insert
    VP64-NtcA-2xNLS_SV40
    Use
    Lentiviral and Synthetic Biology
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    NtcA R90E
    Promoter
    EF1a
    Available Since
    July 17, 2026
    Availability
    Academic Institutions and Nonprofits only
    254308-plasmid-map-sequence-id-508527

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  14. pLenti EF1a VP64-NtcA_R90E-2xNLS_SV40

    Plasmid
    #254309
    Purpose
    Constitutive expression of VP64- NtcA_R90E-2xNLS_SV40 for use in the inactive aKG-ON biosensor. Requires pLenti Neo glnA PCC 6803 reporter gene plasmid.
    Depositor
    Samuel McBrayer
    Article
    Sternisha et al Science. 2026 Jul 16;393(6808):eadx8675.
    Insert
    VP64-NtcA_R90E-2xNLS_SV40
    Use
    Lentiviral and Synthetic Biology
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    NtcA R90E
    Promoter
    EF1a
    Available Since
    July 17, 2026
    Availability
    Academic Institutions and Nonprofits only
    254309-plasmid-map-sequence-id-508533

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  15. pUBC-SV40NLS-PopZ3H-QtE-TSapphire

    Plasmid
    #250610
    Purpose
    Mammalian lentiviral vector for the expression of the Sapphire-tagged nuclear localizing 50nm nucQtE-GEMs under the UBC promoter
    Depositor
    Liam Holt
    Insert
    Encapsulin
    Use
    Lentiviral
    Tags
    PopZTag and TSapphire
    Expression
    Bacterial and Mammalian
    Mutation
    SV40NLS signal on N-terminal
    Available Since
    Jan. 28, 2026
    Availability
    Academic Institutions and Nonprofits only
    250610-plasmid-map-sequence-id-498552

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  18. pTL54_pyCAG-s36GFPmNG-t2A-mCherry3NLS-polyA

    Plasmid
    #223546
    Purpose
    Minimal PUFFFIN cassette with s36GFP-mNeonGreen and mCherry-3NLS, separated by t2A self-cleaving peptide, strong constitutive promoter CAG, no insulation or mammalian selection gene
    Depositor
    Sally Lowell
    Article
    Lebek et al EMBO J. 2024 Jul 12. doi: 10.1038/s44318
    Insert
    s36GFP-mNeonGreen, mCherry-3NLS
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Jan. 21, 2026
    Availability
    Academic Institutions and Nonprofits only
    223546-plasmid-map-sequence-id-442169

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Showing: 3681 - 3700 of 6447 results