We narrowed to 10,636 results for: t7

1. pS2GG

   Plasmid

   #108198

   Purpose

   S. solfataricus PCNA2

   Depositor

   Teruyuki Nagamune

   Article

   Iwata et al Biotechnol J. 2018 Nov;13(11):e1700662.

   Insert

   S. solfataricus PCNA2
   

   Tags

   His6 tag

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   April 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pM2GG

   Plasmid

   #108201

   Purpose

   M. sedula PCNA2

   Depositor

   Teruyuki Nagamune

   Article

   Iwata et al Biotechnol J. 2018 Nov;13(11):e1700662.

   Insert

   M. sedula PCNA2
   

   Tags

   His6 tag

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   April 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pM3GG

   Plasmid

   #108202

   Purpose

   M. sedula PCNA3

   Depositor

   Teruyuki Nagamune

   Article

   Iwata et al Biotechnol J. 2018 Nov;13(11):e1700662.

   Insert

   M. sedula PCNA3
   

   Tags

   His6 tag

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. 2QO2 (EPHA3)

   Plasmid

   #101271

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   EPHA3 (EPHA3 Human)
   

   Tags

   Hexahistidine tag with Thrombin cleavage

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 2, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pJC20Tpm2

   Plasmid

   #99356

   Purpose

   Bacterial expression of budding yeast (S. cerevisiae) Tropomyosin, Tpm2

   Depositor

   Dan Mulvihill

   Article

   Skoumpla et al J Cell Sci. 2007 May 1;120(Pt 9):1635-45

   Insert

   Tropomyosin (TPM2 Budding Yeast)
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pITRSF4

   Plasmid

   #89699

   Purpose

   For production of GFP with "GLP" N-tag and "TRNGL" for C-tag for cyclisation control.

   Depositor

   Hideo Iwai

   Article

   Mikula et al FEBS Lett. 2017 Mar 30. doi: 10.1002/187

   Insert

   SMT3_GLP_H6_GFP_TRNGL
   

   Tags

   SMT3

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJTRSF50

   Plasmid

   #89700

   Purpose

   For production of GFP with "GLP" N-tag and "TRNCL" for C-tag for cyclisation control.

   Depositor

   Hideo Iwai

   Article

   Mikula et al FEBS Lett. 2017 Mar 30. doi: 10.1002/187

   Insert

   SMT3_GLP_H6_GFP_TRNCL
   

   Tags

   SMT3

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET15b-E228A-PvuRts1I

   Plasmid

   #86896

   Purpose

   express E228A variant PvuRts1I in ER2566 strain

   Depositor

   Matthias Bochtler

   Article

   Kazrani et al Nucleic Acids Res. 2014 May;42(9):5929-3

   Insert

   PvuRts1I (pvuRts1I )
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   E228A

   Promoter

   T7

   Available Since

   Feb. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET15b-W215A-PvuRts1I

   Plasmid

   #86898

   Purpose

   express W215A variant PvuRts1I in ER2566 strain

   Depositor

   Matthias Bochtler

   Article

   Kazrani et al Nucleic Acids Res. 2014 May;42(9):5929-3

   Insert

   PvuRts1I (pvuRts1I )
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   W215A

   Promoter

   T7

   Available Since

   Feb. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28a-PvuRts1I

    Plasmid

    #86888

    Purpose

    express C-term His tagged PvuRts1I in ER2566 strain

    Depositor

    Matthias Bochtler

    Article

    Kazrani et al Nucleic Acids Res. 2014 May;42(9):5929-3

    Insert

    PvuRts1I (pvuRts1I )
    

    Tags

    C-term His tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET15b-E71A-PvuRts1I

    Plasmid

    #86895

    Purpose

    express E71A variant PvuRts1I in ER2566 strain

    Depositor

    Matthias Bochtler

    Article

    Kazrani et al Nucleic Acids Res. 2014 May;42(9):5929-3

    Insert

    PvuRts1I (pvuRts1I )
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    E71A

    Promoter

    T7

    Available Since

    Feb. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET15b-D57A-PvuRts1I

    Plasmid

    #86892

    Purpose

    express D57A variant PvuRts1I in ER2566 strain

    Depositor

    Matthias Bochtler

    Article

    Kazrani et al Nucleic Acids Res. 2014 May;42(9):5929-3

    Insert

    PvuRts1I (pvuRts1I )
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    D57A

    Promoter

    T7

    Available Since

    Feb. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET15b-R208A-PvuRts1I

    Plasmid

    #86891

    Purpose

    express R208A variant PvuRts1I in ER2566 strain

    Depositor

    Matthias Bochtler

    Article

    Kazrani et al Nucleic Acids Res. 2014 May;42(9):5929-3

    Insert

    PvuRts1I (pvuRts1I )
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    R208A

    Promoter

    T7

    Available Since

    Feb. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET15b-E20A-PvuRts1I

    Plasmid

    #86897

    Purpose

    express E20A variant PvuRts1I in ER2566 strain

    Depositor

    Matthias Bochtler

    Article

    Kazrani et al Nucleic Acids Res. 2014 May;42(9):5929-3

    Insert

    Pvurts1I (pvuRts1I )
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    E20A

    Promoter

    T7

    Available Since

    Feb. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET15b-D70A-PvuRts1I

    Plasmid

    #86890

    Purpose

    express D70A variant PvuRts1I in ER2566 strain

    Depositor

    Matthias Bochtler

    Article

    Kazrani et al Nucleic Acids Res. 2014 May;42(9):5929-3

    Insert

    PvuRts1I (pvuRts1I )
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    D70A

    Promoter

    T7

    Available Since

    Feb. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJK566

    Plasmid

    #71586

    Purpose

    Produces E. coli acetyl-CoA:oxalate CoA-transferase (yfdE) (YfdE)

    Depositor

    T. J. Kappock

    Article

    Mullins et al PLoS One. 2013 Jul 23;8(7):e67901. doi:

    Insert

    acetyl-CoA:oxalate CoA-transferase (yfdE )
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJK593

    Plasmid

    #71587

    Purpose

    Produces E. coli acetyl-CoA:oxalate CoA-transferase (yfdE) with N-terminal His6 tag (H6YfdE)

    Depositor

    T. J. Kappock

    Article

    Mullins et al PLoS One. 2013 Jul 23;8(7):e67901. doi:

    Insert

    acetyl-CoA:oxalate CoA-transferase (yfdE )
    

    Tags

    His6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJK290

    Plasmid

    #71554

    Purpose

    Production of Acetobacter aceti 1023 alanine racemase (alr) with double mutant (AaAlr-K39A,Y260A)

    Depositor

    T. J. Kappock

    Article

    Francois et al Protein Expr Purif. 2007 Jan;51(1):39-48

    Insert

    alanine racemase
    

    Expression

    Bacterial

    Mutation

    changes lysine-39 to alanine and tyrosine-260 to …

    Promoter

    T7

    Available Since

    March 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJK598

    Plasmid

    #72523

    Purpose

    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase subunits, one with C-terminal His6 tag, and associated hypothetical protein (AaPurS, AaOrfY, AaPurQ, AaPurL)

    Depositor

    T. J. Kappock

    Inserts

    formylglycinamidine ribonucleotide synthetase, small subunit
    hypothetical protein in purSQL operon
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    synthetic gene

    Promoter

    T7 and n/a

    Available Since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJK326

    Plasmid

    #71557

    Purpose

    Production of Acetobacter aceti 1023 alanine racemase (alr) with N-terminal His tag and Ala368>Asp mutant (H6AaAlr-A368D)

    Depositor

    T. J. Kappock

    Article

    Francois et al Protein Expr Purif. 2007 Jan;51(1):39-48

    Insert

    alanine racemase
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    changes alanine-368 to aspartate

    Promoter

    T7

    Available Since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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