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pMRXIB-WIPI2b-mRuby3

Plasmid

#221749

Purpose

Stable expression of WIPI2b-mRuby3 in mammalian cells

Depositor

Noboru Mizushima

Article

Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

Insert

WIPI2b (WIPI2 Human)

Use

Retroviral

Tags

mRuby3

Expression

Mammalian

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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CVS-N2c-EGFP-FlpO

Plasmid

#172373

Purpose

Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.

Depositor

Yoav Ben-Simon, Peter Jonas

Article

Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

Insert

EGFP + FlpO

Use

Neurotropic virus

Available Since

Sept. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-NanoLuc-15aa linker-βarrestin2-no HA-GS linker-TEVp

Plasmid

#125235

Purpose

Expresses SPARK2 NanoLuc-βarrestin2-TEVp (no HA) in mammalian cells

Depositor

Alice Ting

Article

Kim et al eLife. 2019 Apr 3;8. pii: 43826. doi: 10

Insert

NanoLuc-15aa linker-βarrestin2-no HA-GS linker-TEVp

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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His SUMO TEV WT SPOP 28-359

Plasmid

#194115

Purpose

Wild type His-SUMO SPOP expression plasmid

Depositor

Tanja Mittag

Article

Thomasen et al Elife. 2023 Mar 1;12:e84147. doi: 10.755

Insert

SPOP (SPOP Human)

Tags

6His-SUMO-TEV

Expression

Bacterial

Available Since

Feb. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-RT007-GCN4scFV-VB

Plasmid

#163387

Purpose

2nd generation lentiviral vector to express anti-GCN4 module in the G-baToN system

Depositor

Monte Winslow

Article

Tang et al Elife. 2020 Oct 7;9. pii: 61080. doi: 10

Insert

antiGCN4scFV-VEEGFRtm-BFP (GCN4scFV-VB)

Use

Lentiviral

Tags

BFP

Expression

Bacterial and Mammalian

Promoter

EF1a

Available Since

Jan. 11, 2021

Availability

Academic Institutions and Nonprofits only

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CSII-IDR2-chimpTRIM5-FOS

Plasmid

#79065

Purpose

Express chimpanzee TRIM5alpha in mammalan cells

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

TRIM5alpha(chimpanzee)-FOS

Tags

OneSTrEP-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-Flp-DOG-NW

Plasmid

#75469

Purpose

Flp recombinase dependent on GFP (Flp-DOG) for rAAV production and expression in mammalian cells

Depositor

Connie Cepko

Article

Tang et al Elife. 2016 May 20;5. pii: e15312. doi:

Has Service

AAV1

Insert

Flp-DOG

Use

AAV and Synthetic Biology

Expression

Mammalian

Promoter

EF-1a

Available Since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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pUC57-PB-PGK-tetR-3xFLAG-HA-2A-Puro

Plasmid

#129537

Purpose

PiggyBac transposon carrying tetR-3xFLAG-HA-2A-Puro under the PGK promoter.

Depositor

Hideyuki Okano

Article

Tsujimura et al Elife. 2020 May 5;9. pii: 47980. doi: 10

Insert

tetR-3xFlag-HA-2A-Puro

Use

Synthetic Biology

Promoter

hPGK promoter

Available Since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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VinTS-tCRMod-GGSGGS7

Plasmid

#111764

Purpose

Expresses vinculin tension sensor comprised of Clover-(GGSGGS)7-mRuby2 module

Depositor

Brenton Hoffman

Article

LaCroix et al eLife. 2018 Jul 19;7. pii: 33927. doi: 1

Insert

Clover-mRuby2-based vinculin tension sensor with (GGSGGS)7 extensible domain

Expression

Mammalian

Mutation

Residues 1-228 of Clover, residues 1-3 deleted fr…

Promoter

CMV

Available Since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

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pGI3EM18

Plasmid

#135484

Purpose

Agrobacterium T-DNA with SpunH2Bpr driving hph-tdTomato

Depositor

Nicolas Buchler

Article

Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

Insert

SpunH2Bpr hph-tdTomato ADH1t

Tags

tdTomato

Expression

Bacterial and Yeast

Promoter

SpunH2Bpr

Available Since

May 19, 2020

Availability

Academic Institutions and Nonprofits only

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