We narrowed to 7,456 results for: Ski;

1. pMXS-IRES-Blast POLE1 C-ISCmut

   Plasmid

   #160804

   Purpose

   Express C-terminal ISC mutant POLE1

   Depositor

   Richard Possemato

   Article

   Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

   Insert

   POLE1 C-ISCmut (POLE Human)
   

   Use

   Retroviral

   Tags

   3xFLAG

   Expression

   Mutation

   shPOLE_1 and shPOLE2 resistant, C2221S, C2224S, C…

   Promoter

   Available Since

   Oct. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJEP312-pAAV-CMV-SaCas9-P2A-HAFLAGHA-KASH-pA

   Plasmid

   #113689

   Purpose

   SaCas9 driven by CMV. SaCas9 is followed by the self cleaving P2A sequence, several tags, and then the KASH transmembrane domain to enable FACS

   Depositor

   Jonathan Ploski

   Article

   Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

   Insert

   SaCas9 (NEWENTRY )
   

   Use

   AAV and CRISPR

   Tags

   Flag, HAx2, KASH, and NLS

   Expression

   Mutation

   Promoter

   Cytomegalo Virus(CMV)

   Available Since

   Dec. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEF5/FRT/DEST-3xHA/mGli3/Flag P5-6A

   Plasmid

   #51249

   Purpose

   Encodes N-3xHA-TEV-mouseGli3-Flag-C; amino acids 980,1006 mutated to Ala

   Depositor

   Rajat Rohatgi

   Article

   Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

   Insert

   Gli3 (Gli3 Mouse)
   

   Use

   Flpin system

   Tags

   3xHA-TEV and Flag

   Expression

   Mammalian

   Mutation

   amino acids 980,1006 mutated to Ala

   Promoter

   EF1a

   Available Since

   Feb. 28, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pQC MCS IRES G418

   Plasmid

   #110344

   Purpose

   γ-Retroviral transfer vector for cloning and expressing your gene of interest. IRES-driven Geneticin selection.

   Depositor

   Sandra Martha Gomes Dias

   Article

   Adamoski et al Nat Struct Mol Biol. 2023 Dec;30(12):190

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEF5/FRT/DEST-3xHA/mGli3/Flag P1-4A

   Plasmid

   #51247

   Purpose

   Encodes N-3xHA-TEV-mouseGli3-Flag-C; amino acids 849,865,877,907 mutated to Ala

   Depositor

   Rajat Rohatgi

   Article

   Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

   Insert

   Gli3 (Gli3 Mouse)
   

   Use

   Flpin system

   Tags

   3xHA-TEV and Flag

   Expression

   Mammalian

   Mutation

   amino acids 849,865,877,907 mutated to Ala

   Promoter

   EF1a

   Available Since

   Feb. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pMXS-IRES-Blast POLD1 ISCmut

   Plasmid

   #160806

   Purpose

   Express ISC mutant POLD1

   Depositor

   Richard Possemato

   Article

   Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

   Insert

   POLD1 ISCmut (POLD1 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Codon optimized C-terminal 230 amino acids, C1058…

   Promoter

   Available Since

   Oct. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCW-TAZ-CAMTA1

   Plasmid

   #235681

   Purpose

   Express TAZ-CAMTA1 fusion gene in mammalian cells using the doxycycline-inducible TRE promoter

   Depositor

   Duojia Pan

   Article

   Driskill et al Genes Dev. 2021 Apr 1;35(7-8):495-511. d

   Insert

   TAZ-CAMTA1 (WWTR1, CAMTA1 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAK-DR30(SapI)-hfCas13d-Puro-pA/EF1A-mCherry-WPRE-pA

   Plasmid

   #233046

   Purpose

   To express a RfxCas13d-compatible gRNA and to express HA Tagged hfCas13d and a Puro resistance gene. It also encodes an mCherry gene. The CasRx and Puro coding regions are separated by a T2A site.

   Depositor

   Jonathan Ploski

   Article

   Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

   Insert

   RfxCas13d-compatible gRNA expression cassette and hfCas13d and mCherry
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAK-DR30(SapI)-CasRx-Puro-pA/EF1A-mCherry-WPRE-pA

   Plasmid

   #233045

   Purpose

   To express a RfxCas13d-compatible gRNA and to express HA Tagged CasRx and a Puro resistance gene. It also encodes an mCherry gene. The CasRx and Puro coding regions are separated by a T2A site.

   Depositor

   Jonathan Ploski

   Article

   Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

   Insert

   RfxCas13d-compatible gRNA expression cassette and CasRx and mCherry
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. LENTICRISPR-GPI_sgRNA2

    Plasmid

    #201595

    Purpose

    CRISPR/Cas9-mediated gene knock-out

    Depositor

    Vamsi Mootha, Alexis Jourdain

    Article

    Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

    Insert

    GPI (GPI Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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