We narrowed to 7,223 results for: ALP

1. pCMV-XPACK-DNAJC5 (L115R)- HaloTag

   Plasmid

   #205712

   Purpose

   Mammalian expression of XPACK-DNAJC5 (L115R)-HaloTag

   Depositor

   Randy Schekman

   Article

   Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

   Insert

   DNAJC5 (DNAJC5 Human)
   

   Use

   Tags

   HaloTag and XPACK (GCINSKRKD)

   Expression

   Mammalian

   Mutation

   L115R

   Promoter

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-DEAD XPACK-DNAJC5 (L115R)

   Plasmid

   #205718

   Purpose

   Mammalian expression of DEAD XPACK-DNAJC5 (L115R)

   Depositor

   Randy Schekman

   Article

   Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

   Insert

   DNAJC5 (DNAJC5 Human)
   

   Use

   Tags

   Dead XPACK (GCINIKRKD)

   Expression

   Mammalian

   Mutation

   L115R

   Promoter

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pVITRO1-HAPPID1

   Plasmid

   #204626

   Purpose

   Expresses the human anti-Phl p 7 IgD/λ antibody 102.1F10 (HAPPID1) in mammalian cells. HAPPID1 binds to the grass pollen allergen Phl p 7 with subnanomolar affinity.

   Depositor

   James McDonnell

   Article

   Vester et al Antibodies (Basel). 2023 Nov 16;12(4):75

   Inserts

   HAPPID1 heavy chain
   HAPPID1 light chain
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mouse elongation factor 1 alpha and rat elongatio…

   Available since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pOTTC1751 - pcDNA3.1 CMV-IE V5-ER-LBD

   Plasmid

   #201818

   Purpose

   A plasmid expressing the ligand-binding domain of estrogen receptor with a V5 epitope tag from a CMV promoter

   Depositor

   Christopher Richie

   Article

   Bonaventura et al Sci Transl Med. 2023 Jul 26;15(706):eadd

   Insert

   V5-ER-LBD
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-IE

   Available since

   Oct. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. cacna2d1 HA D491A pcDNA3

   Plasmid

   #206070

   Purpose

   expression of rat calcium channel alpha2delta-1 with a single HA tag inserted after aa549 and a D491A mutation that prevents Gabapentin binding

   Depositor

   Annette Dolphin

   Article

   Gumerov et al Proc Natl Acad Sci U S A. 2022 Mar 8;119

   Insert

   cacna2d1 (Cacna2d1 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D491A

   Promoter

   CMV

   Available since

   Sept. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. TFORF2598

   Plasmid

   #144212

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   RORA (RORA Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET15b MOSPD2 RD/LD [282-490]

   Plasmid

   #186470

   Purpose

   Expression of human MOSPD2 (MSP domain from residue 282 to 490; mutant R404D/L406D, unable to bind FFAT motifs), fused to a 6HIS tag, in bacteria

   Depositor

   Catherine Tomasetto

   Article

   Di Mattia et al EMBO Rep. 2018 Jul;19(7). pii: embr.2017

   Insert

   MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)
   

   Use

   Tags

   6HIS

   Expression

   Bacterial

   Mutation

   MSP domain [282-490]

   Promoter

   Available since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. 3020_pETcon-SARS-CoV-2-RBD_N501Y

   Plasmid

   #184405

   Purpose

   yeast surface display of the SARS-CoV-2 Alpha variant RBD

   Depositor

   Jesse Bloom

   Article

   Starr et al Science. 2022 Jun 28:eabo7896. doi: 10.1

   Insert

   SARS-CoV-2 Alpha Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)
   

   Use

   Tags

   HA and c-Myc

   Expression

   Yeast

   Mutation

   N501Y

   Promoter

   Available since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. SNN-GFP

   Plasmid

   #163664

   Purpose

   Expresses a TNF and ST6GAL1 swapped chimera with the cytosolic tail from ST6GAL1, and TMD and luminal domain from TNF in mammalian cells

   Depositor

   Lei Lu

   Article

   Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

   Insert

   ST6 beta-galactoside alpha-2,6-sialyltransferase 1 and tumor necrosis factor (ST6GAL1, TNF Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   This chimera contains the cytosolic tail from ST,…

   Promoter

   Available since

   Nov. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. NNS-GFP

    Plasmid

    #163666

    Purpose

    Expresses a TNF and ST6GAL1 swapped chimera with the cytosolic tail and TMD from TNF, and luminal domain from ST6GAL1 in mammalian cells

    Depositor

    Lei Lu

    Article

    Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

    Insert

    ST6 beta-galactoside alpha-2,6-sialyltransferase 1 and tumor necrosis factor (ST6GAL1, TNF Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    This chimera contains the cytosolic tail from TNF…

    Promoter

    Available since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. SITS-EGFP-Bin1a

    Plasmid

    #176020

    Purpose

    Leishmania cell free expression of zebrafish EGFP-Bin1a. Parton lab clone GRQ

    Depositor

    Rob Parton

    Article

    Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

    Insert

    Bin1a
    

    Use

    Leishmania cell free expression

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    Available since

    Nov. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. SITS-mCherry-Bin1a

    Plasmid

    #176024

    Purpose

    Leishmania cell free expression of zebrafish mCherry-Bin1a. Parton lab clone GSS

    Depositor

    Rob Parton

    Article

    Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

    Insert

    Bin1a
    

    Use

    Leishmania cell free expression

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Available since

    Nov. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV_Donor/Reporter-COL4A3

    Plasmid

    #130279

    Purpose

    COL4A3 mutation-specific sgRNA under the control of U6 promoter; mCherry-sgRNA target-(out of frame)EGFP expression cassette; COL4A3 donor template

    Depositor

    Silvestro Conticello

    Article

    Daga et al Eur J Hum Genet. 2019 Nov 21. pii: 10.10

    Insert

    mCherry-eGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV_COL4A3_self-cleavingCas9

    Plasmid

    #130281

    Purpose

    Expresses an spCas9 under the control of a CMV promoter; The spCas9 is flanked by COL4A3 sgRNA targets to induce its self-cleaving and inactivation

    Depositor

    Silvestro Conticello

    Article

    Daga et al Eur J Hum Genet. 2019 Nov 21. pii: 10.10

    Insert

    spCas9
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV_COL4A5_self-cleavingCas9

    Plasmid

    #130282

    Purpose

    Expresses an spCas9 under the control of a CMV promoter; The spCas9 is flanked by COL4A5 sgRNA targets to induce its self-cleaving and inactivation

    Depositor

    Silvestro Conticello

    Article

    Daga et al Eur J Hum Genet. 2019 Nov 21. pii: 10.10

    Insert

    spCas9
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-HA-DEPTOR S286/287/291A

    Plasmid

    #159160

    Purpose

    Expresses DEPTOR S286/287/291A mutant in mammalian cells

    Depositor

    Michele Pagano

    Article

    Duan et al Mol Cell. 2011 Oct 21;44(2):317-24. doi:

    Insert

    DEPTOR (DEPTOR Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    changed serine 286,287 and 291 to alanine

    Promoter

    CMV

    Available since

    Sept. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-HA-DEPTOR S286/287/291D

    Plasmid

    #159161

    Purpose

    Expresses DEPTOR S286/287/291D mutant in mammalian cells

    Depositor

    Michele Pagano

    Article

    Duan et al Mol Cell. 2011 Oct 21;44(2):317-24. doi:

    Insert

    DEPTOR (DEPTOR Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    changed serine 286,287 and 291 to aspartic acid

    Promoter

    CMV

    Available since

    Sept. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRC/CMV-TYK2(delta)TK-VSV

    Plasmid

    #139353

    Purpose

    Expression of TYK2 protein missing the tyrosine kinase (TK) domain (aa 1-895)

    Depositor

    Sandra Pellegrini

    Article

    Velazquez et al J Biol Chem. 1995 Feb 17;270(7):3327-34.

    Insert

    TYK2 cDNA expressing aa 1-895 (TYK2 Human)
    

    Use

    Tags

    VSV

    Expression

    Mammalian

    Mutation

    V362F-deletion of aa 896-1187

    Promoter

    CMV

    Available since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. hKCNB1 L211P

    Plasmid

    #124487

    Purpose

    missense mutation in children with EOEE. HA tagged in C-terminus, pCIneo vector

    Depositor

    Federico Sesti

    Article

    Yu et al FASEB J. 2019 Dec;33(12):14680-14689. do

    Insert

    human KCNB1 (KCNB1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    changed leucine 211 to proline

    Promoter

    Available since

    Sept. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. hKCNB1 R312H

    Plasmid

    #124488

    Purpose

    missense mutation in children with EOEE. HA tagged in C-terminus, pCIneo vector

    Depositor

    Federico Sesti

    Article

    Yu et al FASEB J. 2019 Dec;33(12):14680-14689. do

    Insert

    human KCNB1 (KCNB1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    changed arginine 312 to histidine

    Promoter

    Available since

    Sept. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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