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hSgk1_v3 Flag

Plasmid

#86039

Purpose

full length human Sgk1_i3 with Flag tag

Depositor

Christie Thomas

Article

Raikwar et al Am J Physiol Renal Physiol. 2012 Dec 1;3

Insert

Alternate transcript v3 of human Sgk1 (SGK1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

v3; S313G

Promoter

CMV

Available Since

April 7, 2017

Availability

Academic Institutions and Nonprofits only

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pSec7-Halox2

Plasmid

#115425

Purpose

Express Sec7-Halox2 at the endogenous locus via pop-in pop-out mutagenesis

Depositor

Benjamin Glick

Article

Casler et al J Cell Biol. 2019 May 6;218(5):1582-1601

Insert

Sec7-Halox2

Tags

HaloTag

Expression

Yeast

Promoter

Endogenous

Available Since

Nov. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-Nck1 (M3)

Plasmid

#45896

Depositor

Louise Larose

Article

Kebache et al Proc Natl Acad Sci U S A. 2002 Apr 16;99

Insert

Nck-1 M3 mutant (NCK1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

M3 (W229R) in SH3 domain

Promoter

CMV

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only
pUC-UAS1B8-TEF

Plasmid

#44374

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B8-TEF promoter

Use

Synthetic Biology

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B8-TEF

Available Since

April 17, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pZCAG

Plasmid

#129114

Purpose

Z. rouxii centromeric vector containing G418 resistance

Depositor

Paolo Giudici

Article

Bizzarri et al Yeast. 2019 Dec;36(12):711-722. doi: 10.

Type

Empty backbone

Use

Cre/Lox

Expression

Yeast

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-Nck1 (SH2 mut)

Plasmid

#45893

Depositor

Louise Larose

Article

Kebache et al Proc Natl Acad Sci U S A. 2002 Apr 16;99

Insert

Nck-1 SH2 mutant (NCK1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

in SH2 domain (R308K) and E302K and Q333H

Promoter

CMV

Available Since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only
pUC-UAS1B20-Leum

Plasmid

#44325

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B20-Leum promoter

Use

Synthetic Biology

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B20-Leum

Available Since

April 18, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pZEG

Plasmid

#129112

Purpose

Z. rouxii episomal vector containing G418 resistance

Depositor

Paolo Giudici

Article

Bizzarri et al Yeast. 2019 Dec;36(12):711-722. doi: 10.

Type

Empty backbone

Use

Cre/Lox

Expression

Yeast

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pUC-UAS1B16-TEF(1004)

Plasmid

#44365

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B16-TEF(1004) promoter

Use

Synthetic Biology

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B16-TEF(1004)

Available Since

July 15, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pcryBFP_Umyo18bGFP

Plasmid

#172527

Purpose

alphaA-crystallin promoter (cry) drives GFP expression in the lens. The 600bp unc-45b muscle-specific promoter drives expression of GFP-tagged zebrafish Myo18b.

Depositor

Joachim Berger

Article

Berger et al Hum Mol Genet. 2017 Mar 15;26(6):1146-11

Insert

myo18b

Expression

Mammalian

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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EF2451_pET21a(+)

Plasmid

#13840

Depositor

SeWon Suh

Article

Kang et al Acta Crystallograph Sect F Struct Biol C

Insert

PPAT

Tags

His

Expression

Bacterial

Available Since

June 29, 2007

Availability

Academic Institutions and Nonprofits only

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pAS1_4xh7SKPylT_EF1_BsmBI_Intein_DD

Plasmid

#162810

Purpose

STELLA destination plasmid containing restriction sites for golden gate cloning. Suitable for amber suppression (4xMmaPylT cassette); for transient expression

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

BsmBI_Intein_DD

Use

Piggybac

Expression

Mammalian

Promoter

EF1alpha

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAS1_4x7SKPylT_EF1_Ub-*GFP

Plasmid

#162806

Purpose

STELLA plasmid for amber suppression of N-terminally labelled GFP

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

EF1_Ub-(N-TAG)GFP

Use

Piggybac

Expression

Mammalian

Mutation

GFP (Met1,Amber)

Promoter

EF1alpha

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pARiBo1

Plasmid

#98892

Purpose

Expresses ARiBo-fused RNA in vitro with T7 RNA polymerase

Depositor

Pascale Legault

Article

Di Tomasso et al Nucleic Acids Res. 2011 Feb;39(3):e18. d

Insert

ARiBo1

Use

In vitro transcription vector

Promoter

T7 promoter

Available Since

Aug. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pSec7-iGFPx6

Plasmid

#115424

Purpose

Express Sec7-iGFPx6 at the endogenous locus via pop-in pop-out mutagenesis

Depositor

Benjamin Glick

Article

Casler et al J Cell Biol. 2019 May 6;218(5):1582-1601

Insert

Sec7-iGFPx6

Tags

iGFP

Expression

Yeast

Promoter

Endogenous

Available Since

Nov. 21, 2018

Availability

Academic Institutions and Nonprofits only

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LI C-D 11GFP

Plasmid

#54431

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

superfolder GFP 11 domain

Use

Cloning vector

Available Since

Nov. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pUC-UAS1B8-TEF(1004)

Plasmid

#44366

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B8-TEF(1004) promoter

Use

Synthetic Biology

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B8-TEF(1004)

Available Since

July 15, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pDHJS3 AN-

Plasmid

#78241

Purpose

One of four plasmids needed to create the mismatched double Holliday junction substrate (MM-DHJS).

Depositor

Tao-shih Hsieh

Article

Plank et al J Biol Chem. 2006 Jun 23;281(25):17510-6

Inserts

loxP
sequence "A" with NheI site
loxP2

Available Since

Sept. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pUC-UAS1B6-Leum

Plasmid

#44314

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B6-Leum promoter

Use

Synthetic Biology

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B6-Leum

Available Since

April 18, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pDHJS3 AS-

Plasmid

#78237

Purpose

One of four plasmids needed to create the double Holliday junction substrate (DHJS).

Depositor

Tao-shih Hsieh

Article

Plank et al J Biol Chem. 2006 Jun 23;281(25):17510-6

Inserts

loxP
sequence "B"
loxP2

Use

Phagemid

Available Since

Sept. 26, 2016

Availability

Academic Institutions and Nonprofits only

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Human NCKX4-AS-short (pcDNA3.1+)

Plasmid

#75210

Purpose

Mammalian expression of SLC24A4

Depositor

Jonathan Lytton

Article

Li et al J Biol Chem. 2002 Dec 13;277(50):48410-7

Insert

SLC24A4 (SLC24A4 Human)

Expression

Mammalian

Mutation

alternatively spliced exon absent

Promoter

CMV

Available Since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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pGST1-yGGA1-VHS

Plasmid

#44421

Depositor

James Hurley

Article

Ren et al EMBO J. 2010 Mar 17;29(6):1045-54. Epub

Insert

yGGA1-VHS (GGA1 Budding Yeast)

Tags

GST

Expression

Bacterial

Available Since

July 29, 2013

Availability

Academic Institutions and Nonprofits only
pUC-UAS1B12-Leum

Plasmid

#44320

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B12-Leum promoter

Use

Synthetic Biology

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B12-Leum

Available Since

April 17, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pUC-UAS1B5-Leum

Plasmid

#44313

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B5-Leum promoter

Use

Synthetic Biology

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B5-Leum

Available Since

July 15, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pUC-UAS1B10-Leum

Plasmid

#44318

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B10-Leum promoter

Use

Synthetic Biology

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B10-Leum

Available Since

April 18, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pUC-UAS1B8-TEF(203)

Plasmid

#44378

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B8-TEF(203) promoter

Use

Synthetic Biology

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B8-TEF(203)

Available Since

April 18, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pUC-UAS1B8-TEF(804)

Plasmid

#44368

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B8-TEF(804) promoter

Use

Synthetic Biology

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B8-TEF(804)

Available Since

April 18, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pBL155

Plasmid

#246591

Purpose

express cCadC AtRbcL fusion protein

Depositor

Tina Wang

Article

Li et al Proc Natl Acad Sci U S A. 2025 Sep 16;12

Insert

cCadC-AtRbcL (rbcL Synthetic)

Expression

Bacterial

Available Since

Nov. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pRO288

Plasmid

#233827

Purpose

MoClo-YTK; type4A; Activation domains; VP48

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

VP48

Expression

Yeast

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pRO129

Plasmid

#233805

Purpose

MoClo-YTK; type3; rtTA systems; rtTA3

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

rtTA3

Expression

Yeast

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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