We narrowed to 7,201 results for: mme

1. pTYB1-pLAC-V69S

   Plasmid

   #48721

   Purpose

   Bacterial expression of Lacritin Protein with Mutation at protein 69 from Valine to Serine

   Depositor

   Gordon Laurie

   Article

   Wang et al J Biol Chem. 2013 Jun 21;288(25):18146-6

   Insert

   Lacritin (LACRT Human)
   

   Tags

   Intein

   Expression

   Bacterial

   Mutation

   Valine 69 to Serine

   Promoter

   T7

   Available Since

   Oct. 18, 2013

   Availability

   Academic Institutions and Nonprofits only

2. pCR4-*mRFP

   Plasmid

   #47118

   Purpose

   Can be used to make an RNA probe for whole-mount in situ hybridization, for in-frame fusion proteins or as a subcloning step for mRFP. Note that the coding sequence for mRFP lacks the start codon.

   Depositor

   Stephen Ekker

   Article

   Clark et al Nat Methods. 2011 Jun;8(6):506-15. Epub

   Insert

   *mRFP
   

   Expression

   Bacterial

   Mutation

   No start codon

   Promoter

   Lac

   Available Since

   Oct. 16, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDM10

   Plasmid

   #41759

   Depositor

   Jeff Gelles

   Article

   Martin et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

   Insert

   kinesin heavy chain (Khc E. coli, Fly)
   

   Tags

   His6 and bccp

   Expression

   Bacterial

   Mutation

   residues 1-401; C45S C338S A378C C451S

   Available Since

   July 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDM8

   Plasmid

   #41758

   Depositor

   Jeff Gelles

   Article

   Martin et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

   Insert

   kinesin heavy chain (Khc E. coli, Fly)
   

   Tags

   His6 and bccp

   Expression

   Bacterial

   Mutation

   residues 1-401; C45S C338S S364C C451S

   Available Since

   July 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pOU1

   Plasmid

   #41753

   Depositor

   Jeff Gelles

   Article

   Martin et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

   Insert

   kinesin heavy chain (Khc E. coli, Fly)
   

   Tags

   His6 and bccp

   Expression

   Bacterial

   Mutation

   residues 1-401; C338S C451S

   Available Since

   July 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. 5' Del 5

   Plasmid

   #16053

   Depositor

   Robert Benezra

   Article

   Tournay et al Mol Cell Biol. 1996 May . 16(5):2418-30.

   Insert

   Id1 promoter, 5' deletion (Id1 Mouse)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   -527 to +88 of promoter

   Available Since

   Nov. 2, 2007

   Availability

   Academic Institutions and Nonprofits only

7. 5' Del 7

   Plasmid

   #16055

   Depositor

   Robert Benezra

   Article

   Tournay et al Mol Cell Biol. 1996 May . 16(5):2418-30.

   Insert

   Id1 promoter, 5' deletion (Id1 Mouse)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   -127 to +88 of promoter

   Available Since

   Nov. 2, 2007

   Availability

   Academic Institutions and Nonprofits only

8. 5' Del 8

   Plasmid

   #16056

   Depositor

   Robert Benezra

   Article

   Tournay et al Mol Cell Biol. 1996 May . 16(5):2418-30.

   Insert

   Id1 promoter, 5' deletion (Id1 Mouse)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   -52 to +88 of promoter

   Available Since

   Nov. 2, 2007

   Availability

   Academic Institutions and Nonprofits only

9. pAAV-hSyn-FLEX-TeLC-P2A-dTomato

   Plasmid

   #159102

   Purpose

   Cre-dependent tetanus toxin light chain construct with nuclear-localized dTomato fluorophore for conditional silencing of neurons.

   Depositor

   Sandeep Datta

   Article

   Pashkovski et al Nature. 2020 Jul;583(7815):253-258. doi:

   Insert

   TeLC-P2A-NLS-dTomato
   

   Use

   AAV

   Promoter

   hSynapsin

   Available Since

   Nov. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHR_PGK_SNIPR_Humanized Hinge Notch SNIPR (Pax6)

    Plasmid

    #188383

    Purpose

    Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch2-JMD, and a Pax6(DBD)-p65(428-551) transcriptional factor

    Depositor

    Kole Roybal

    Article

    Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

    Insert

    PGK_antiCD19_CD8alpha-variant2-ECD_Notch1-TMD_Notch2-JMD_Pax6(DBD)-p65(428-551)
    

    Use

    Lentiviral

    Available Since

    March 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. SpRYc

    Plasmid

    #175575

    Purpose

    Expresses the codon-optimized, engineered SpRYc Cas9 nuclease in mammalian cells

    Depositor

    Joseph Jacobson

    Article

    Koseki et al Res Sq. 2023 Mar 7:rs.3.rs-2625838. doi:

    Insert

    SpRYc
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    PID of SpRY grafted onto the N-terminal domain of…

    Promoter

    CMV

    Available Since

    May 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLX_305_Ovalbumin

    Plasmid

    #184924

    Purpose

    Lentiviral vector for expressing Ovalbumin. Also expresses the puromycin resistance gene as a selectable marker.

    Depositor

    Arlene Sharpe

    Article

    Milling et al J Exp Med. 2024 Apr 1;221(4):e20230699.

    Insert

    Ovalbumin (OVAL Chicken)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    Our vector has an A188T mutation that does not im…

    Promoter

    PGK

    Available Since

    Feb. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. CROPseq-Guide-Puro

    Plasmid

    #86708

    Purpose

    Makes CRISPR gRNAs detectable by single-cell RNA-seq. The gRNA is expressed as part of the puromycin resistance mRNA transcribed by RNA Pol II and in its functional form from the hU6 promoter.

    Depositor

    Christoph Bock

    Article

    Datlinger et al Nat Methods. 2017 Jan 18. doi: 10.1038/n

    Insert

    EF1a-Puro-WPRE-hU6-gRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Feb. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. tetO.Sox9.Puro

    Plasmid

    #117269

    Purpose

    Expresses Sox9 and puromycin resistance gene under control of tetO sequence.

    Depositor

    Henrik Ahlenius

    Article

    Canals et al Nat Methods. 2018 Sep;15(9):693-696. doi

    Insert

    Sox9 (Sox9 Mouse)
    

    Use

    Lentiviral

    Tags

    T2A and puromycin resistant cassette (C terminal …

    Expression

    Mammalian

    Promoter

    tet-on

    Available Since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. tetO.Nfib.Hygro

    Plasmid

    #117271

    Purpose

    Expresses Nfib and hygromycin resistance gene under control of tetO sequence.

    Depositor

    Henrik Ahlenius

    Article

    Canals et al Nat Methods. 2018 Sep;15(9):693-696. doi

    Insert

    Nfib (Nfib Mouse)
    

    Use

    Lentiviral

    Tags

    IRES and hygromycin resistant cassette (C termina…

    Expression

    Mammalian

    Promoter

    tet-on

    Available Since

    Nov. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLEX_305-N-dTAG

    Plasmid

    #91797

    Purpose

    Lentiviral/gateway cloning vector for N-terminally tagging proteins of interest with the dTAG system

    Depositor

    Behnam Nabet, James Bradner

    Article

    Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    FKBP F36V

    Expression

    Mammalian

    Promoter

    hPGK

    Available Since

    March 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLViP-LifeAct-iRFP670

    Plasmid

    #229699

    Purpose

    Lentiviral expression of LifeAct-iRFP670 in mammalian cells

    Depositor

    Alpha Yap

    Article

    Noordstra et al Dev Cell. 2023 Sep 25;58(18):1748-1763.e

    Insert

    Synthetic construct LifeAct-mTurquoise gene
    

    Use

    Lentiviral

    Tags

    iRFP670

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLViP-LifeAct-TagRFPT

    Plasmid

    #229700

    Purpose

    Lentiviral expression of LifeAct-TagRFPT in mammalian cells

    Depositor

    Alpha Yap

    Article

    Noordstra et al Dev Cell. 2023 Sep 25;58(18):1748-1763.e

    Insert

    Synthetic construct LifeAct-mTurquoise gene
    

    Use

    Lentiviral

    Tags

    TagRFPT

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. GSN-bio-His

    Plasmid

    #52067

    Purpose

    Expresses full-length Gelsolin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    GSN (GSN Human)
    

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. 10279-M28-663

    Plasmid

    #225662

    Purpose

    Lentivirus protein expression. Matched control construct for 8522-M01-663 (Addgene # 225660) gives basal expression from mCMVp for gating/thresholding

    Depositor

    Dominic Esposito

    Article

    Tang et al Stem Cell Reports. 2015 Jan 13;4(1):155-

    Insert

    dsCopGFP
    

    Use

    Lentiviral

    Promoter

    mCMVp

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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