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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 9,415 results for: Coli

Showing: 9301 - 9350 of 9415 results

  1. pET-NPR4 (SA binding domain)

    Plasmid
    #226885
    Purpose
    Expresses the Salicylic acid binding core (SBC) of NPR4 in E.coli, in an insoluble form
    Depositor
    Ning Zheng
    Article
    Wang et al Nature. 2020 Oct;586(7828):311-316. doi:
    Insert
    The Salicylic acid (SA) binding domain of NPR4 (NPR4 Mustard Weed)
    Use
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    NPR4 SA-binding core (residues 373-516), with a s…
    Promoter
    T7
    Available Since
    Oct. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    226885-plasmid-map-sequence-id-449178

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  5. pET-Duet-6xHis-TEV-Nsp8(76-198)

    Plasmid
    #201024
    Purpose
    Bacterial expression of codon optimized SARS-CoV-2 nsp8 residues 76-198, with an N-terminus His tag followed by a TEV cleavage site.
    Depositor
    Sharon Rozovsky
    Article
    Ghelichkhani et al J Mol Biol. 2023 Apr 15;435(8):168008. d
    Insert
    non-structural protein 8 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
    Use
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Mutation
    Gene insert is codon optimized for Ecoli.
    Promoter
    T7/LacO
    Available Since
    May 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    201024-plasmid-map-sequence-id-394447

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  6. pET-DEST42-dre4

    Plasmid
    #170440
    Purpose
    Express drosophila dre4 in E.coli expression system. Generated from pDONR-dre4 by Gateway system. Used for custom antibody purification.
    Depositor
    Joan Conaway
    Article
    Tettey et al Cell Rep. 2019 Jun 25;27(13):3770-3779.e
    Insert
    dre4 (dre4 Fly)
    Use
    Tags
    6xHis and V5
    Expression
    Bacterial
    Mutation
    Deleted amino acids 1-20
    Promoter
    T7
    Available Since
    July 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    170440-plasmid-map-sequence-id-338015

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  18. pGEX6P1-3xMBT

    Plasmid
    #46987
    Purpose
    Inducible expression of 3xMBT from L3MBTL1 as a GST fusion protein in E.coli
    Depositor
    Or Gozani
    Article
    Moore et al Mol Cell. 2013 May 9;50(3):444-56. doi:
    Insert
    Triple MBT domain from human L3MBTL1 (L3MBTL1 Human)
    Use
    Tags
    GST and PreScission cleavage site
    Expression
    Bacterial
    Mutation
    contains amino acids 190–530 of accession NP_0562…
    Promoter
    tac
    Available Since
    Sept. 10, 2013
    Availability
    Academic Institutions and Nonprofits only
    46987-plasmid-map-sequence-id-66939

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  23. pMG024 MBP-GSK3β-HA-His, GST_λPPase

    Plasmid
    #196182
    Purpose
    Co-expresses MBP-GSK3β-HA-His (human GSK3β as a fusion protein with MBP, HA, and His-tags) and GST_λPPase in E.coli to produce unphosphorylated MBP-GSK3β-HA-His
    Depositor
    Jesse Zalatan
    Article
    Gavagan et al Elife. 2023 Aug 7;12:e85444. doi: 10.755
    Inserts
    GSK3B (GSK3B Human)
    λPPase
    Use
    Tags
    GST, HA, His6, and MBP
    Expression
    Bacterial
    Mutation
    Promoter
    Tac
    Available Since
    Jan. 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    196182-plasmid-map-sequence-id-383259

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  27. pETM33_Nsp15

    Plasmid
    #156481
    Purpose
    Bacterial expression of Sars-CoV2 Nsp15 protein with His-tag and GST-tag
    Depositor
    Ylva Ivarsson
    Article
    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:
    Insert
    Nsp15 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
    Use
    Tags
    His-GST
    Expression
    Bacterial
    Mutation
    Gene insert is codon optimized for Ecoli.
    Promoter
    T7/LacO
    Available Since
    Aug. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    156481-plasmid-map-sequence-id-307733

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  28. pGEX6P1-3xMBT_D355N

    Plasmid
    #46988
    Purpose
    Inducible expression of 3xMBT (D355N) from L3MBTL1 as a GST fusion protein in E.coli
    Depositor
    Or Gozani
    Article
    Moore et al Mol Cell. 2013 May 9;50(3):444-56. doi:
    Insert
    Triple MBT domain from human L3MBTL1 (L3MBTL1 Human)
    Use
    Tags
    GST and PreScission cleavage site
    Expression
    Bacterial
    Mutation
    Mutation aspartic acid 355 to asparagine (D355N);…
    Promoter
    tac
    Available Since
    Sept. 10, 2013
    Availability
    Academic Institutions and Nonprofits only
    46988-plasmid-map-sequence-id-66963

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  29. pETM33_Nsp10

    Plasmid
    #156479
    Purpose
    Bacterial expression of Sars-CoV2 Nsp10 protein with His-tag and GST-tag
    Depositor
    Ylva Ivarsson
    Article
    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:
    Insert
    Nsp10 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
    Use
    Tags
    His-GST
    Expression
    Bacterial
    Mutation
    Gene insert is codon optimized for Ecoli.
    Promoter
    T7/LacO
    Available Since
    Aug. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    156479-plasmid-map-sequence-id-307731

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  31. pETM33_Nsp4

    Plasmid
    #156474
    Purpose
    Bacterial expression of Sars-CoV2 Nsp4 protein with His-tag and GST-tag
    Depositor
    Ylva Ivarsson
    Article
    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:
    Insert
    Nsp4 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
    Use
    Tags
    His-GST
    Expression
    Bacterial
    Mutation
    Gene insert is codon optimized for Ecoli.
    Promoter
    T7/LacO
    Available Since
    Aug. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    156474-plasmid-map-sequence-id-307734

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  33. pETM33_Nsp16

    Plasmid
    #156482
    Purpose
    Bacterial expression of Sars-CoV2 Nsp16 protein with His-tag and GST-tag
    Depositor
    Ylva Ivarsson
    Article
    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:
    Insert
    Nsp16 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
    Use
    Tags
    His-GST
    Expression
    Bacterial
    Mutation
    Gene insert is codon optimized for Ecoli.
    Promoter
    T7/LacO
    Available Since
    Aug. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    156482-plasmid-map-sequence-id-307736

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  36. pETM33_Nsp9

    Plasmid
    #156478
    Purpose
    Bacterial expression of Sars-CoV2 Nsp9 protein with His-tag and GST-tag
    Depositor
    Ylva Ivarsson
    Article
    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:
    Insert
    Nsp9 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
    Use
    Tags
    His-GST
    Expression
    Bacterial
    Mutation
    Gene insert is codon optimized for Ecoli.
    Promoter
    T7/LacO
    Available Since
    Aug. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    156478-plasmid-map-sequence-id-307616

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  41. pETM33_Nsp3d_Ub2_PLPro

    Plasmid
    #156472
    Purpose
    Bacterial expression of Sars-CoV2 Nsp3d_Ub2_PLPro protein with His-tag and GST-tag
    Depositor
    Ylva Ivarsson
    Article
    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:
    Insert
    Nsp3d_Ub2_PLPro (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
    Use
    Tags
    His-GST
    Expression
    Bacterial
    Mutation
    Gene insert is codon optimized for Ecoli.
    Promoter
    T7/LacO
    Available Since
    Aug. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    156472-plasmid-map-sequence-id-307744

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  42. pETM33_Nsp1

    Plasmid
    #156465
    Purpose
    Bacterial expression of Sars-CoV2 Nsp1 protein with His-tag and GST-tag
    Depositor
    Ylva Ivarsson
    Article
    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:
    Insert
    Nsp1 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
    Use
    Tags
    His-GST
    Expression
    Bacterial
    Mutation
    Gene insert is codon optimized for Ecoli.
    Promoter
    T7/LacO
    Available Since
    Aug. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    156465-plasmid-map-sequence-id-307729

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  44. pETM33_N_CTD

    Plasmid
    #156484
    Purpose
    Bacterial expression of Sars-CoV2 N_CTD protein with His-tag and GST-tag
    Depositor
    Ylva Ivarsson
    Article
    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:
    Insert
    N_CTD (N Severe acute respiratory syndrome coronavirus 2, Synthetic)
    Use
    Tags
    His-GST
    Expression
    Bacterial
    Mutation
    Gene insert is codon optimized for Ecoli.
    Promoter
    T7/LacO
    Available Since
    Aug. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    156484-plasmid-map-sequence-id-307735

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  46. pETM33_N_NTD

    Plasmid
    #156483
    Purpose
    Bacterial expression of Sars-CoV2 N_NTD protein with His-tag and GST-tag
    Depositor
    Ylva Ivarsson
    Article
    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:
    Insert
    N_NTD (N Severe acute respiratory syndrome coronavirus 2, Synthetic)
    Use
    Tags
    His-GST
    Expression
    Bacterial
    Mutation
    Gene insert is codon optimized for Ecoli.
    Promoter
    T7/LacO
    Available Since
    Aug. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    156483-plasmid-map-sequence-id-307745

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  50. pETM33_Nsp7

    Plasmid
    #156476
    Purpose
    Bacterial expression of Sars-CoV2 Nsp7 protein with His-tag and GST-tag
    Depositor
    Ylva Ivarsson
    Article
    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:
    Insert
    Nsp7 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
    Use
    Tags
    His-GST
    Expression
    Bacterial
    Mutation
    Gene insert is codon optimized for Ecoli.
    Promoter
    T7/LacO
    Available Since
    Aug. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    156476-plasmid-map-sequence-id-307742

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Showing: 9301 - 9350 of 9415 results