We narrowed to 7,241 results for: CIT

1. pBABE-α-Syn-nYFP

   Plasmid

   #92203

   Purpose

   Retroviral overexpression vector for α-Syn bimolecular fluorescence complementation (BiFC)

   Depositor

   Huda Zoghbi

   Article

   Rousseaux et al Elife. 2016 Oct 25;5. pii: e19809. doi:

   Insert

   SNCA (SNCA Human)
   

   Use

   Retroviral

   Tags

   YFP(1-155)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. 61426-hU6-shmFmr1-EF1a-mCherry

   Plasmid

   #157859

   Purpose

   Expresses a shRNA targeting mouse Fmr1 gene

   Depositor

   Xinyu Zhao

   Article

   Shen et al Nat Neurosci. 2019 Mar;22(3):386-400. do

   Insert

   Fmr1 (Fmr1 Mouse)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-EF1α1.1-FLPX-rc [Chronos-GFP]

   Plasmid

   #122102

   Purpose

   AAV-mediated expression of Chronos-GFP under the EF1α1.1 promoter in frt/reversed (Flp-dependent) manner.

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   Chronos-GFP
   

   Use

   AAV

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a（1.1kb short version)

   Available since

   Sept. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCEP4-myc-EFNB2 (D62Q-Q130L-V167L)

   Plasmid

   #200977

   Purpose

   Mammalian expression plasmid for myc-tagged EFNB2 (D62Q-Q130L-V167L specificity mutant)

   Depositor

   Erik Procko

   Article

   Narayanan et al J Virol. 2023 Nov 30;97(11):e0062123. do

   Insert

   EFNB2 (EFNB2 Human)
   

   Use

   Tags

   Extracellular c-myc epitope tag and Signal peptid…

   Expression

   Mammalian

   Mutation

   D62Q-Q130L-V167L (increase specificity towards he…

   Promoter

   CMV

   Available since

   Oct. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-EFNB2-RBD-Fc (D62Q-Q130L-V167L)

   Plasmid

   #200980

   Purpose

   Mammalian expression plasmid for soluble EFNB2 (D62Q-Q130L-V167L specificity mutant) fused to IgG1 Fc

   Depositor

   Erik Procko

   Article

   Narayanan et al J Virol. 2023 Nov 30;97(11):e0062123. do

   Insert

   EFNB2 (EFNB2 Human)
   

   Use

   Tags

   IgG1 Fc

   Expression

   Mammalian

   Mutation

   Soluble Receptor-Binding Domain; D62Q-Q130L-V167L…

   Promoter

   CMV

   Available since

   Oct. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSPL3-hRPH3A_c.1853Gmut

   Plasmid

   #190479

   Purpose

   minigene assay

   Depositor

   Alfredo Brusco

   Article

   Pavinato et al Genet Med. 2023 Nov;25(11):100922. doi:

   Insert

   RPH3A (NM_014954.3) c.1853 [exon 20 and part of surrounding introns only] (RPH3A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   c.1853A>G

   Promoter

   SV40

   Available since

   Sept. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. CMV-ShadowY-CBD(H83L/H86L)-P2A-Clover(T153M/F223R)-Cdc42

   Plasmid

   #165436

   Purpose

   Expresses ShadowY-CBD(H83L/H86L) and Clover(T153M/F223R)-Cdc42 in mammalian cells as a negative control for FLIM imaging.

   Depositor

   Hideji Murakoshi

   Article

   Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

   Insert

   ShadowY-CBD(H83L/H86L)-P2A-Clover(T153M/F223R)-Cdc42
   

   Use

   Tags

   ShadowY, Clover(T153M/F223R)

   Expression

   Mammalian

   Mutation

   H83L/H86L: CDB(Cdc42 Binding Domain, Pak3, residu…

   Promoter

   CMV

   Available since

   March 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEX4T2-Ste7-EE

   Plasmid

   #52681

   Purpose

   expression of constitutively active Ste7 kinase in bacteria

   Depositor

   Benjamin Turk

   Article

   Mok et al Sci Signal. 2010 Feb 16;3(109):ra12. doi

   Insert

   Ste7 (STE7 Budding Yeast)
   

   Use

   Tags

   GST and Myc

   Expression

   Bacterial

   Mutation

   S359E/T363E constitutively active

   Promoter

   tac

   Available since

   April 30, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pLVX LYCHOS (GPR155) WT-FLAG

   Plasmid

   #199633

   Purpose

   Lentiviral construct to stably express LYCHOS (GPR155) WT

   Depositor

   Roberto Zoncu

   Article

   Shin et al Science. 2022 Sep 16;377(6612):1290-1298

   Insert

   GPR155 (GPR155 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   May 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAV-PGK-Chst3

    Plasmid

    #193024

    Purpose

    AAV vector plasmid expressing mouse chondroitin 6-sulfotransferase (Chst3) under the mouse phosphoglycerate kinase (PGK) promoter

    Depositor

    Bart Nieuwenhuis

    Article

    Yang et al Mol Psychiatry. 2021 Jul 16. pii: 10.103

    Insert

    Chst3 (Chst3 Mouse)
    

    Use

    AAV

    Tags

    3xFLAG and myc-tag

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSN840

    Plasmid

    #184832

    Purpose

    Expresses human KIF5A(∆exon27) and human KLC1 in insect cells

    Depositor

    Kyoko Chiba

    Article

    Nakano et al Genes Cells. 2022 Apr 17. doi: 10.1111/g

    Inserts

    KIF5A (∆exon27) (KIF5A Human)
    KLC1 (KLC1 Human)
    

    Use

    Cre/Lox

    Tags

    His-FLAG and mScarlet-2xStrepII

    Expression

    Insect

    Mutation

    ∆exon27 form of human KIF5A

    Promoter

    p10 and polyhedrin

    Available since

    July 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. Pvalb-2A-Cre targeting vector

    Plasmid

    #61570

    Purpose

    Target the Cre recombinase gene to the stop codon of the mouse Pvalb gene

    Depositor

    Hongkui Zeng

    Article

    Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

    Insert

    Pvalb exon 4 - 2A - Cre
    

    Use

    Cre/Lox and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. Pvalb-2A-Flpo Flp-in replacement vector

    Plasmid

    #61572

    Purpose

    Recombinase-mediated cassette exchange in ES cells to insert the Flpo recombinase gene at the mouse Pvalb gene stop codon

    Depositor

    Hongkui Zeng

    Article

    Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

    Insert

    Pvalb-2A-Flpo
    

    Use

    Recombinase-mediated cassette exchange using flp …

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-EF1α-FLEX-rc[Chronos-GFP]

    Plasmid

    #62725

    Purpose

    AAV-mediated expression of Chronos-GFP under the EF1α promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    Chronos-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1α

    Available since

    April 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDGB3 alpha1 U6-26:gRNA 4 (Pnos):2.1 aptamer (GB1724)

    Plasmid

    #160621

    Purpose

    Target for the Nopaline Synthetase Promoter with the MS2 recognition 2.1 loop in position3' in the scaffold

    Depositor

    Diego Orzaez

    Article

    Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

    Insert

    U6-26:gRNA 4 (pNos): 2.1 aptamer
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    U6-26

    Available since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. Snap25-LSL-2A-GFP targeting vector

    Plasmid

    #61575

    Purpose

    Target a Cre-dependent GFP expression cassette to the stop codon of the mouse Snap25 gene

    Depositor

    Hongkui Zeng

    Article

    Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

    Insert

    Snap25-LSL-2A-GFP
    

    Use

    Cre/Lox and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSN25

    Plasmid

    #100110

    Purpose

    expresses a chimeric SOX2-repressor fusion

    Depositor

    Mark Ptashne

    Article

    Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

    Insert

    mCitrine-P2A-SOX2 fused to full length HP1 alpha (SOX2, CBX5 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. AAV pTRE-FSF-FLEX-EGFP-WPRE-bGHpA

    Plasmid

    #65453

    Purpose

    Can be used to generate AAV virus that will express EGFP from the tetO promoter under intersectional control by Flp and Cre recombinases

    Depositor

    Hongkui Zeng

    Article

    Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

    Has Service

    AAV1

    Insert

    EGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    tetO

    Available since

    Jan. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. PX458-3xHA-SpCas9

    Plasmid

    #130968

    Purpose

    3xHA tagged Cas9 from S. pyogenes with T2A-EGFP and cloning backbone for sgRNA

    Depositor

    Debojyoti Chakraborty

    Article

    Acharya et al Proc Natl Acad Sci U S A. 2019 Oct 15;11

    Insert

    3xHA-NLS-SpCas9
    

    Use

    CRISPR

    Tags

    3x HA, EGFP, and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available since

    Nov. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMG023 MBP-Axin 384-518-His (miniAxin)

    Plasmid

    #154012

    Purpose

    Expresses MBP-Axin_384-518-His (human Axin minimal scaffold fragment as a fusion protein with MBP and His- tags) in E.coli

    Depositor

    Jesse Zalatan

    Article

    Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

    Insert

    Axin1 (AXIN1 Human)
    

    Use

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    381-518 fragment

    Promoter

    Tac

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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