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172_pAAV-ProC37-CatCh-GFP-WPRE

Plasmid

#125971

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

ProC37

Available since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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173_pAAV-ProC38-CatCh-GFP-WPRE

Plasmid

#125972

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

ProC38

Available since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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174_pAAV-ProC39-CatCh-GFP-WPRE

Plasmid

#125973

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

ProC39

Available since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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42_pAAV-ProC41-CatCh-GFP-WPRE

Plasmid

#125975

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

ProC41

Available since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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157_pAAV-ProD2-CatCh-GFP-WPRE

Plasmid

#125978

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

ProD2

Available since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pJEP13-AAV-H1/TO-L-sgRNA(Tet2)-CMV-TetR-P2A-eGFP-KASH-pA

Plasmid

#82703

Purpose

AAV backbone with a full length H1/TO promoter driving expression of a sgRNA that targets exon 3 of the mouse Tet2 locus. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.

Depositor

Jonathan Ploski

Article

de Solis et al Front Mol Neurosci. 2016 Aug 18;9:70. do

Insert

H1/TO-L gRNA Expression Cassette Containing Tet2 gRNA

Use

AAV and CRISPR

Tags

CMV promoter driving expression of TetR-P2A-GFP-K…

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 20, 2016

Availability

Academic Institutions and Nonprofits only

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EW265 SFFV-mCherry-SGc(stem-1(3xPUF9R-tar))cSG-EGFP (FLP-IN)

Plasmid

#236124

Purpose

Plasmid encoding fluorescent reporter for PUF-9R binding which expresses mCherry constitutively and EGFP upon stop codon deamination due to ADAR-PUF9R binding

Depositor

Xiaojing Gao

Article

Wolfsberg et al bioRxiv 2025.02.24.639918

Insert

mCherry-SGc(stem-1(3xPUF9R-tar))cSG-EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-SV40NLSf-GFP-3xmiR122-WPRE-HGHpA

Plasmid

#183775

Purpose

AAV genome with a CAG driven eGFP reporter with mR122 target sequence repeats to reduce transgene expression in hepatocytes.

Depositor

Ben Deverman

Article

Krolak et al Nat Cardiovasc Res. 2022 Apr;1(4):389-40

Insert

NLS-GFP

Use

AAV

Tags

Expression

Mutation

NA

Promoter

CAG

Available since

May 2, 2022

Availability

Academic Institutions and Nonprofits only

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pCDH-EF1-E-NoMi-P2A-CopGFP-T2A-PuroR

Plasmid

#207805

Purpose

E-NoMi is a re-engineered tetraspanin scaffold tagged with bioluminescent and fluorescent reporter proteins under an EF-1α promoter.

Depositor

Koen Breyne

Article

Maalouf et al Mol Ther. 2023 Jul 5;31(7):2206-2219. do

Insert

E-NoMi

Use

Lentiviral

Tags

3xFlag tag, copGFP, mCherry, and nanoluciferase

Expression

Mammalian

Mutation

Promoter

EF-1alpha

Available since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-FLEX-macaque OPN4-IRES-EGFP

Plasmid

#223253

Purpose

AAV construct for Cre-dependent (FLEX) expression of macaque melanopsin (Opn4, short isoform, GenBank:OP856641; hSyn promoter) and of EGFP via an IRES.

Depositor

Michael Do

Article

Liu et al Science. 2023 Jan 27;379(6630):376-381.

Insert

macaque OPN4-IRES-EGFP (OPN4 )

Use

AAV

Tags

Expression

Mutation

Promoter

human Synapsin 1 (hSyn)

Available since

Nov. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GFP-IRES-hSNCA (A11P/V70P)

Plasmid

#185717

Purpose

AAV expression of GFP and human α-Synuclein with A11P and V70P mutations from hSyn promoter

Depositor

Jun Ding

Article

Albarran et al Nat Neurosci. 2023 Jun;26(6):997-1007. d

Insert

synuclein, alpha (SNCA Human)

Use

AAV

Tags

Expression

Mutation

Changed Ala 11 to Pro, Val 70 to Pro

Promoter

hSyn

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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Tol2-pA-GI-TagRFP-E1b-5xUAS-E1b-eGFP-GI-pA-Tol2; gcryst:CFP

Plasmid

#135812

Purpose

This plasmid can be used to express independently eGFP and TagRFP from a bidirectional 5xUAS. Also CFP expression under crystallin promoter.

Depositor

Nadia Mercader Huber

Article

Sanz-Morejon et al Cell Rep. 2019 Jul 30;28(5):1296-1306.e6

Insert

TagRFP

Use

Zebrafish

Tags

Expression

Mutation

Promoter

UAS

Available since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-Archon1-KGC-GFP-ER2

Plasmid

#115892

Purpose

AAV-mediated expression of Archon1-KGC-GFP-ER2 under the Syn promoter. Using bGHpA signal.

Depositor

Edward Boyden

Article

Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

Has Service

AAV8

Insert

Archon1-KGC-EGFP-ER2

Use

AAV

Tags

ER2, GFP, and KGC

Expression

Mammalian

Mutation

Promoter

Syn

Available since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-1BPNLS-Cas9-1BPNLS-2AGFP

Plasmid

#87109

Purpose

BPNLS-Cas9-BPNLS-2AGFP expression plasmid

Depositor

Juan Belmonte

Article

Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

Insert

1BPNLS-Cas9-1BPNLS-2A-EGFP

Use

CRISPR

Tags

3xFLAG (on Cas9)

Expression

Mammalian

Mutation

Promoter

CAG

Available since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO {mCAR}off-{DTR-GFP}on-WPRE

Plasmid

#111395

Purpose

AAV vector with hSynapsin promoter, Cre-OFF mCAR (for efficient CAV-2 infection), Cre-ON DTR-EGFP (diphtheria toxin receptor for cell ablation)

Depositor

Adam Kepecs

Article

Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi

Inserts

mCAR (Cxadr Mouse)
DTR-EGFP

Use

AAV

Tags

EGFP and Myc

Expression

Mammalian

Mutation

Promoter

hSynapsin

Available since

June 8, 2018

Availability

Academic Institutions and Nonprofits only

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PL752-OTX2-LA-p2a-EGFPnls-hGHpA-PGK-puroR-OTX2-RA

Plasmid

#191690

Purpose

For knocking p2a-EGFP into OTX2 locus

Depositor

Yuejun Chen

Article

Xu et al J Clin Invest. 2022 Jul 15;132(14). pii:

Insert

OTX2 homology arms and p2a-EGFP

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

Available since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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PL452-CLSTN2-LA-p2a-EGFP-hGHpA-PGK-neoR-CLSTN2-RA

Plasmid

#191688

Purpose

For knocking p2a-EFGP into CLSTN2 locus

Depositor

Yuejun Chen

Article

Xu et al J Clin Invest. 2022 Jul 15;132(14). pii:

Insert

CLSTN2 homology arms and p2a-EGFP

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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PL452-PTPRO-LA-p2a-EGFP-hGHpA-PGK-neoR-PTPRO-RA

Plasmid

#191689

Purpose

For knocking p2a-EGFP into PTPRO locus

Depositor

Yuejun Chen

Article

Xu et al J Clin Invest. 2022 Jul 15;132(14). pii:

Insert

PTPRO homology arms and p2a-EGFP

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pHR-EF1a-Archon1-KGC-EGFP-ER2

Plasmid

#108425

Purpose

Lentivirus production plasmid encoding for Archon1 fluorescent voltage reporter

Depositor

Edward Boyden

Article

Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

Insert

Archon1-KGC-EGFP-ER2

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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pHR-EF1a-Archon2-KGC-EGFP-ER2

Plasmid

#108426

Purpose

Lentivirus production plasmid encoding for Archon2 fluorescent voltage reporter

Depositor

Edward Boyden

Article

Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

Insert

Archon2-KGC-EGFP-ER2

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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