We narrowed to 4,088 results for: PRS

1. KBB416

   Plasmid

   #185087

   Purpose

   Swi6L-GFP with nuclear export signal mutated to alanines

   Depositor

   Ken Belanger

   Article

   Belanger et al BMC Mol Cell Biol. 2022 Feb 21;23(1):10.

   Insert

   SWI6
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Swi6 nuclear export signal L to A mutations

   Promoter

   Available since

   July 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. KBB394

   Plasmid

   #185086

   Purpose

   NMA111 with both nuclear localization signals mutated to alanines fused to GFP (mutagenesis of KBB280)

   Depositor

   Ken Belanger

   Article

   Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

   Insert

   NMA111
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Nma111 KKR 9-11 AAA; KRK 28-30 AAA

   Promoter

   Available since

   July 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. KBB349

   Plasmid

   #185080

   Purpose

   rfa2D248 truncation fused to GFP

   Depositor

   Ken Belanger

   Article

   Belanger et al DNA Cell Biol. 2011 Sep;30(9):641-51. do

   Insert

   RFA2
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Rfa2 truncation aa 1-247

   Promoter

   Available since

   July 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. KBB353

   Plasmid

   #185081

   Purpose

   Swi6M-GFP expressing N-term 181 amino acids of Swi6

   Depositor

   Ken Belanger

   Article

   Belanger et al BMC Mol Cell Biol. 2022 Feb 21;23(1):10.

   Insert

   SWI6
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Swi6 truncation aa 1-181

   Promoter

   Available since

   July 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. KBB484

   Plasmid

   #185132

   Purpose

   SWI6-GFP full length under control of GAL1 promotion

   Depositor

   Ken Belanger

   Article

   Belanger et al BMC Mol Cell Biol. 2022 Feb 21;23(1):10.

   Insert

   SWI6
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   July 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. CRISPR-psbA2 point mutation

   Plasmid

   #182929

   Purpose

   point mutation on psbA2 by CRISPR in Synechocystis 6803

   Depositor

   Himadri Pakrasi

   Article

   Ungerer et al Sci Rep. 2016 Dec 21;6:39681. doi: 10.10

   Inserts

   ddcpf1
   gRNA targeting psbA2 in Synechocystis 6803: gatcttcggtcgcttgatctttc
   psbA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   S264A, and silent mutation to remove PAM site

   Promoter

   Available since

   April 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. Ecm2 1-197

   Plasmid

   #169924

   Purpose

   WT Ecm2 mutated to introduce stop codon after AA 197 of Ecm2

   Depositor

   Aaron Hoskins

   Article

   van der Feltz et al RNA. 2021 Feb 5. pii: rna.077727.120. do

   Insert

   Ecm2 1-197
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Oct. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBHRSF228

   Plasmid

   #135007

   Purpose

   MusF2 prenyltransferases from Nostoc sp. UHCC 0398

   Depositor

   Hideo Iwai

   Article

   Mattila et al ACS Chem Biol. 2019 Dec 20;14(12):2683-2

   Insert

   MusF2 prenyltransferase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. VanR - F165V

   Plasmid

   #158965

   Purpose

   Wild type VanR where the residue in position 165 was mutated from Phenylalanine to Valine. This mutation abolished the response to vanillic acid. The gene is under the control of the TEF1 promoter.

   Depositor

   Michael Jensen

   Article

   et al Biotechnology Notes, Volume 1, 2020, Pag

   Insert

   VanR - F165V
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Wild type VanR from Caulobacter crescentus with m…

   Promoter

   TEF1

   Available since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. VanR - F165R

    Plasmid

    #158966

    Purpose

    Wild type VanR where the residue in position 165 was mutated from Phenylalanine to Arginine. This mutation abolished the response to vanillic acid. The gene is under the control of the TEF1 promoter.

    Depositor

    Michael Jensen

    Article

    et al Biotechnology Notes, Volume 1, 2020, Pag

    Insert

    VanR - F165R
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Wild type VanR from Caulobacter crescentus with m…

    Promoter

    TEF1

    Available since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMP89b CvTA V124N

    Plasmid

    #141207

    Purpose

    Chromobacterium violaceum transaminase with enhances affinity for PLP. TEV cleaving site after the N-terminal Histag.

    Depositor

    Francesca Paradisi

    Article

    Roura Padrosa et al Front Bioeng Biotechnol. 2019 Oct 18;7:2

    Insert

    CvTA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    V124N

    Promoter

    Available since

    June 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHBDuet139

    Plasmid

    #121913

    Purpose

    Charge-Introduced NpuDnaB mini-intein

    Depositor

    Hideo Iwai

    Article

    Beyer et al FEBS J. 2019 Oct 30. doi: 10.1111/febs.1

    Insert

    CI-NpuDnaBΔ290 intein
    

    Use

    Tags

    GB1 and His6-GB1

    Expression

    Bacterial

    Mutation

    Deletion of 290-residue endonuclease domain, I53K…

    Promoter

    T7

    Available since

    Dec. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHBDuet140

    Plasmid

    #121914

    Purpose

    Orthogonal NpuDnaB mini-intein intein

    Depositor

    Hideo Iwai

    Article

    Beyer et al FEBS J. 2019 Oct 30. doi: 10.1111/febs.1

    Insert

    Oth-NpuDnaBΔ290 intein
    

    Use

    Tags

    GB1 and His6-GB1

    Expression

    Bacterial

    Mutation

    Deletion of 290-residue endonuclease domain, I53K…

    Promoter

    T7

    Available since

    Dec. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMET17p-CCG 316

    Plasmid

    #131166

    Purpose

    For ScMET17 promoter regulated expression of mCherry-Cub-R-GFP (CCG) fused bait proteins, centromeric ARS plasmid, URA3 complements the uracil auxotrophy of S. cerevisiae ura mutant

    Depositor

    Nils Johnsson

    Article

    Dunkler et al Methods Mol Biol. 2015;1346:151-68. doi:

    Type

    Empty backbone

    Use

    Tags

    CCG

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. Spt2-K166R-GFP

    Plasmid

    #115573

    Purpose

    Expresses yeast Spt2-GFP fusion protein mutated from lysine to arginine at site 166

    Depositor

    Michael Downey

    Article

    Rossl et al J Biol Chem. 2019 Apr 19;294(16):6227-62

    Insert

    SPT2
    

    Use

    Tags

    GFP

    Expression

    Bacterial and Yeast

    Mutation

    Lysine 166 mutated to Arginine

    Promoter

    Available since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

16. GFP-X2

    Plasmid

    #115567

    Purpose

    GFP tethered to two repeats of the Gcn5 consensus motif

    Depositor

    Michael Downey

    Article

    Rossl et al J Biol Chem. 2019 Apr 19;294(16):6227-62

    Insert

    Green Fluorescent Protein
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Two repeats of the Gcn5 consensus motif are tagge…

    Promoter

    ADH1

    Available since

    Oct. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. GFP-X1

    Plasmid

    #115566

    Purpose

    GFP tethered to a single repeat of the Gcn5 consensus motif

    Depositor

    Michael Downey

    Article

    Rossl et al J Biol Chem. 2019 Apr 19;294(16):6227-62

    Insert

    Green Fluorescent Protein
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Single Gcn5 consensus motif repeat tethered to C-…

    Promoter

    ADH1

    Available since

    Oct. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Hsh155 N4A 3xFLAG

    Plasmid

    #111975

    Purpose

    Shuffle plasmid containing the HSH155 gene and surrounding regions. It bears a TRP marker. It has 3xFLAG at the C-terminus. Contains the N4A mutation.

    Depositor

    Aaron Hoskins

    Article

    Carrocci et al RNA. 2018 May 11. pii: rna.065664.118. d

    Insert

    Hsh155 N4A 3x FLAG
    

    Use

    Tags

    3xFLAG

    Expression

    Bacterial and Yeast

    Mutation

    T178A R181A R182A R186A

    Promoter

    Available since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. Hsh155 K818A 3xFLAG

    Plasmid

    #111982

    Purpose

    Shuffle plasmid containing the HSH155 gene and surrounding regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has 3xFLAG at the C-terminus. Contains K818A mutation. Lethal

    Depositor

    Aaron Hoskins

    Article

    Carrocci et al RNA. 2018 May 11. pii: rna.065664.118. d

    Insert

    Hsh155 K818A 3x FLAG
    

    Use

    Tags

    3xFLAG

    Expression

    Bacterial and Yeast

    Mutation

    K818A

    Promoter

    Available since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Hsh155 R775A 3xFLAG

    Plasmid

    #111977

    Purpose

    Shuffle plasmid containing the HSH155 gene and surrounding regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has 3xFLAG at the C-terminus. Contains R775A mutation. Lethal

    Depositor

    Aaron Hoskins

    Article

    Carrocci et al RNA. 2018 May 11. pii: rna.065664.118. d

    Insert

    Hsh155 R775A 3x FLAG
    

    Use

    Tags

    3xFLAG

    Expression

    Bacterial and Yeast

    Mutation

    R775A

    Promoter

    Available since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge