159,557 results

1. pBS-CMV-gagpol

   Plasmid

   #35614

   Depositor

   Patrick Salmon

   Insert

   MLV gag and pol genes
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 12, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. TATA-GFP

   Plasmid

   #110833

   Purpose

   negative control

   Depositor

   Michael Lewis

   Article

   Wei et al Stem Cells. 2014 Oct;32(10):2571-82. doi

   Insert

   GFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. His6-TEV-CRBN-midi

   Plasmid

   #215330

   Purpose

   CRBN midi construct for use in crystallography and biophysical studies enabling ligand and degrader design

   Depositor

   Alessio Ciulli

   Article

   Kroupova et al Nat Commun. 2024 Oct 15;15(1):8885. doi:

   Insert

   CRBN (CRBN Human)
   

   Use

   Tags

   6xHis and TEV

   Expression

   Bacterial

   Mutation

   contains amino acids 41-187 followed by a Gly-Ser…

   Promoter

   lacI

   Available since

   March 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSpCas9(BB)-2A-Neo

   Plasmid

   #127762

   Purpose

   Used for expression of sgRNA in mammalian cells

   Depositor

   Ken-Ichi Takemaru

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRRL Luciferase

   Plasmid

   #120798

   Purpose

   For production of lentivirus to express luciferase, primarily for in vivo bioluminescence tracking experiments.

   Depositor

   Paul Khavari

   Article

   Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

   Insert

   Luciferase
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hPGK

   Available since

   Jan. 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLVUT-tTR-KRAB

   Plasmid

   #11651

   Purpose

   Tet-regulated (Tet-on) lentiviral vector for transgene (hUbiquitin promoter) - AND/OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 3rd generation

   Depositor

   Didier Trono, Patrick Aebischer

   Article

   Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

   Has Service

   Cloning Grade DNA

   Insert

   hUbiquitin C, GFP, tTR-KRAB, Tet-on
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 17, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. RED

   Plasmid

   #139835

   Purpose

   Expresses RNase H1-EGFP- dCas9 fusion protein in human cells

   Depositor

   Karim Mekhail

   Article

   Abraham et al Nature. 2020 Sep;585(7824):298-302. doi:

   Insert

   RNase H1-EGFP-dCas9 (RNASEH1 RNase H1 (H. sapiens), dCas9 (S. pyogenes))
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   dCas9 protein contains two mutations: D10A and H8…

   Promoter

   tet-inducible CMV

   Available since

   Sept. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPB-CAG-hCas3

   Plasmid

   #134920

   Purpose

   Components for genome editing in mammalian cells with pCAG-All-in-one-hCascade and pBS-U6-crRNA-targeted.

   Depositor

   Tomoji Mashimo

   Article

   Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:

   Insert

   Cas3 with bpNLS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEBFP2-Nuc

   Plasmid

   #14893

   Purpose

   Mammalian expression of EBFP2 blue fluorescent protein with NLS

   Depositor

   Robert Campbell

   Article

   Ai et al Biochemistry. 2007 Apr 20. ():.

   Insert

   pEBFP2-Nuc
   

   Use

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Replace EYFP of the vector pEYFP-Nuc from Clontec…

   Promoter

   Available since

   May 15, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHR-iCasp9

    Plasmid

    #199441

    Purpose

    Express inducible Caspase9 in mammalian cells

    Depositor

    Chao Tang

    Article

    Yuan et al Cell Death Dis. 2022 Jan 10;13(1):34. do

    Insert

    inducible Caspase9 (CASP9 Human)
    

    Use

    Lentiviral

    Tags

    DmrB and mCherry

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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