We narrowed to 70,757 results for: LAS

1. pPyCAG-Nanog(Q138A)-IP

   Plasmid

   #62997

   Purpose

   Expresses Mouse Nanog Q138A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).

   Depositor

   Shinya Yamanaka

   Insert

   Mouse Nanog Q138A Mutant (Nanog Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Changed Glutamine 138 (139 AA in Mouse Protein) t…

   Promoter

   Available since

   July 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pPyCAG-Nanog(T141A)-IP

   Plasmid

   #62999

   Purpose

   Expresses Mouse Nanog T141A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).

   Depositor

   Shinya Yamanaka

   Insert

   Mouse Nanog T141A Mutant (Nanog Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Changed Threonine 141 (142 AA in Mouse Protein) t…

   Promoter

   Available since

   July 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pPyCAG-Nanog(Q144A)-IP

   Plasmid

   #63000

   Purpose

   Expresses Mouse Nanog Q144A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).

   Depositor

   Shinya Yamanaka

   Insert

   Mouse Nanog Q144A Mutant (Nanog Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Changed Glutamine 144 (145 AA in Mouse Protein) t…

   Promoter

   Available since

   July 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pPyCAG-Nanog(N145A)-IP

   Plasmid

   #63001

   Purpose

   Expresses Mouse Nanog N145A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).

   Depositor

   Shinya Yamanaka

   Insert

   Mouse Nanog N145A Mutant (Nanog Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Changed Asparagine 145 (146 AA in Mouse Protein) …

   Promoter

   Available since

   July 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pPyCAG-Nanog(R147A)-IP

   Plasmid

   #63002

   Purpose

   Expresses Mouse Nanog R147A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).

   Depositor

   Shinya Yamanaka

   Insert

   Mouse Nanog R147A Mutant (Nanog Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Changed Arginine 147 (148 AA in Mouse Protein) to…

   Promoter

   Available since

   July 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pPyCAG-Nanog(K151A)-IP

   Plasmid

   #63004

   Purpose

   Expresses Mouse Nanog K151A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).

   Depositor

   Shinya Yamanaka

   Insert

   Mouse Nanog K151A Mutant (Nanog Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Changed Lysine 151 (152 AA in Mouse Protein) to A…

   Promoter

   Available since

   July 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pPyCAG-Nanog(T100A)-IP

   Plasmid

   #62989

   Purpose

   Expresses Mouse Nanog T100A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).

   Depositor

   Shinya Yamanaka

   Insert

   Mouse Nanog T100A Mutant (Nanog Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Changed Threonine 100 (101AA) to Alanine

   Promoter

   CAG

   Available since

   July 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPyCAG-Nanog(F102A)-IP

   Plasmid

   #62990

   Purpose

   Expresses Mouse Nanog F102A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).

   Depositor

   Shinya Yamanaka

   Insert

   Mouse Nanog F102A Mutant (Nanog Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Changed Phenylalanine 102 (103AA in Mouse Protein…

   Promoter

   Available since

   July 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. PP-MAPK2 (3RP9)

   Plasmid

   #32862

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   MAPK2
   

   Use

   Tags

   His tag with TEV cleavage

   Expression

   Bacterial

   Mutation

   amino acids 123-548 only, P421L

   Promoter

   Available since

   Jan. 10, 2013

   Availability

   Industry, Academic Institutions, and Nonprofits

10. pAAV asyn S129G

    Plasmid

    #36067

    Depositor

    Hilal Lashuel

    Insert

    alpha-synuclein S129G (SNCA Human)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    S129G

    Promoter

    CMV

    Available since

    July 13, 2012

    Availability

    Academic Institutions and Nonprofits only

11. pBAD33-mf-lon

    Plasmid

    #21867

    Depositor

    Robert Sauer

    Article

    Gur et al Proc Natl Acad Sci U S A. 2008 Oct 21. 1

    Insert

    Lon (Mfl404 Mesoplasma florum)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    all TGA codons changed to TGG codons

    Promoter

    Available since

    Aug. 11, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCW57.1 hPHGDH

    Plasmid

    #154916

    Purpose

    Doxycyclin inducible expression of human PHGDH

    Depositor

    Richard Possemato

    Article

    Richard et al Richard Possemato Lab plasmids

    Insert

    PHGDH (PHGDH Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. TRPM4

    Plasmid

    #25903

    Depositor

    Craig Montell

    Article

    Xu et al Proc Natl Acad Sci U S A. 2001 Sep 11. 9

    Insert

    TRPM4 (TRPM4 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pT7RNAP-Q737P

    Plasmid

    #175356

    Purpose

    Support plasmid for T7_ori plasmids

    Depositor

    Sven Panke

    Article

    Becker et al Nucleic Acids Res. 2021 Jul 13. pii: 632

    Insert

    T7RNAP-Q737P
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Q737P

    Promoter

    Available since

    April 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV-CMV-FLEXfrt-SaCas9-U6-sgRosa26

    Plasmid

    #159915

    Purpose

    Mutagenesis of Rosa26

    Depositor

    Larry Zweifel

    Insert

    Rosa26 (Gt(ROSA)26Sor Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pNR4A1.1.0-gDNA

    Plasmid

    #112391

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor NR4A1

    Depositor

    Kevin White

    Insert

    NR4A1 (NR4A1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCAG/HA-hBIG1

    Plasmid

    #226253

    Purpose

    Expresses N-terminally HA-tagged hBIG1 in mammalian cells

    Depositor

    Hye-Won Shin

    Insert

    BIG1 (ARFGEF1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-CMV-FLEX-SaCas9-U6-sgTh

    Plasmid

    #159901

    Purpose

    Mutagenesis of Th

    Depositor

    Larry Zweifel

    Insert

    Th (Th Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMH4-SYN-EGFP-ER

    Plasmid

    #22285

    Depositor

    Thomas Oertner

    Article

    Holbro et al Proc Natl Acad Sci U S A. 2009 Aug 18. (

    Insert

    EGFP-ER
    

    Use

    Tags

    EGFP-ER

    Expression

    Mammalian

    Mutation

    EGFP alanine 206 changed to Lysin

    Promoter

    Available since

    July 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pXZ13lacZalpha

    Plasmid

    #160229

    Purpose

    places LacZalpha unit between Esp3I sites for better cutting of plasmid and ability to distinguish recombinants as white colonies when plated on Xgal plates

    Depositor

    Ian Duncan

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    Oct. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET28-6His-TEV-Nsp14

    Plasmid

    #154954

    Purpose

    Expresses Nsp14 from SARS-CoV-2 with a N-terminal TEV-protease cleavable 6His tag in E. coli

    Depositor

    Justin Kollman

    Insert

    6His-TEV-Nsp14 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

22. pPTbsr

    Plasmid

    #117696

    Purpose

    Blasticidin-S resistance Vector for Phaeodactylum tricornutum

    Depositor

    Peter Kroth

    Article

    Buck et al PeerJ. 2018 Nov 14;6:e5884. doi: 10.7717

    Type

    Empty backbone

    Use

    Vector for phaeodactylum tricornutum

    Tags

    Expression

    Mutation

    Promoter

    fcpB

    Available since

    Dec. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET22-Nsp10-TEV-6His

    Plasmid

    #154957

    Purpose

    Expresses Nsp10 from SARS-CoV-2 with a C-terminal TEV-protease cleavable 6His tag in E. coli

    Depositor

    Justin Kollman

    Insert

    Nsp10-TEV-6His (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    TEV-6His

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    July 21, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

24. pRR-PR-5Z

    Plasmid

    #23057

    Depositor

    Charles Miller

    Article

    Miller et al Plasmid. 2009 Dec 3. ():.

    Insert

    PR (PGR Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 27, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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25. mT2A-sfGFP-linker-iCre-ERT2-FNF

    Plasmid

    #182341

    Purpose

    Recombineering cassette plasmid for T2A-sfGFP-linker-iCre-ERT2-FNF

    Depositor

    Mario Capecchi

    Insert

    T2A-sfGFP-linker-iCre-ERT2-FNF
    

    Use

    Mouse Targeting; Flp / frt

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET28-Nsp14-TEV-6His

    Plasmid

    #154952

    Purpose

    Expresses Nsp14 from SARS-CoV-2 with a C-terminal TEV-protease cleavable 6His tag in E. coli

    Depositor

    Justin Kollman

    Insert

    Nsp14-TEV-6His (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    TEV-6His

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

27. pTEAD1.1.0-gDNA

    Plasmid

    #112425

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor TEAD1

    Depositor

    Kevin White

    Insert

    TEAD1 (TEAD1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pIC144

    Plasmid

    #63067

    Purpose

    Retroviral expression of EGFP-TEV-S-tag CENP-K for protein localization and affinity purification (LAP)

    Depositor

    Iain Cheeseman

    Insert

    CENP-K, IMAGE clone 3511065 (CENPK Human)
    

    Use

    Retroviral

    Tags

    EGFP and TEV-S

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. TRMT10C (RG9MTD1A-c113)

    Plasmid

    #110248

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    RG9MTD1 (TRMT10C Human)
    

    Use

    Tags

    TEV-His6-Flag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 - lacO

    Available since

    June 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. p405MET25

    Plasmid

    #15961

    Depositor

    Fred Cross, Nicolas Buchler

    Insert

    MET25 promoter + polylinker + CYC1 terminator (MET17 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    MET25 promoter is between SacI and XbaI restricti…

    Promoter

    Available since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge