We narrowed to 29,757 results for: REP

1. lenti-SpCas9 blast

   Plasmid

   #104997

   Purpose

   This lentiviral construct delivers hSpCas9 and blasticidin S resistance.

   Depositor

   Brett Stringer

   Article

   Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

   Insert

   KpnI-XhoI-BsrGI-NheI
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAS_4xhyb PylT A41AA C55A CRFR1 95TAG 263TAA-HA

   Plasmid

   #154781

   Purpose

   plasmid with 4xhybrid PylT cassette (mutant A41AA C55A) and rat CRFR1 95TAG 263TAA, for transient transfection or stable, piggybac-mediated, integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

   Insert

   CRFR1 (Crhr1 Rat)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   95TAG 263TAA in CRFR1-HA, hybrid PylT with A41AA …

   Promoter

   EF1

   Available Since

   Sept. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAS_4xhyb PylT A41AA C55A CRFR1 95TAA 263TAG-HA

   Plasmid

   #154780

   Purpose

   plasmid with 4xhybrid PylT cassette (mutant A41AA C55A) and rat CRFR1 95TAA 263TAG, for transient transfection or stable, piggybac-mediated, integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

   Insert

   CRFR1 (Crhr1 Rat)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   95TAA 263TAG in CRFR1-HA, hybrid PylT with A41AA …

   Promoter

   EF1

   Available Since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-CMV-Blast-DNTGFBR2-HA

   Plasmid

   #130888

   Purpose

   Expresses dominant negative mutant human TGFBR2

   Depositor

   Gian-Paolo Dotto

   Article

   Bordignon et al Cell Rep. 2019 Aug 27;28(9):2358-2372.e6

   Insert

   TGFBR2 (TGFBR2 Human)
   

   Use

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   Dominant Negative receptor lacking cytosolic cata…

   Promoter

   CMV

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. CAG-Cas9-T2A-EGFP-ires-puro

   Plasmid

   #78311

   Purpose

   Expresses WT SpCas9, EGFP and Puromycin resistance from a CAG promoter.

   Depositor

   Timo Otonkoski

   Article

   Saarimaki-Vire et al Cell Rep. 2017 Apr 11;19(2):281-294. doi

   Insert

   WT SpCas9-T2A-EGFP-ires-puromycin resistance
   

   Use

   CRISPR

   Tags

   T2A-EGFP-ires-puro

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   April 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCC1-4k-BBclone2

   Plasmid

   #137070

   Purpose

   E. coli plasmid that contains the expression optimized genes for BB0323, P13, DipA and Lmp1

   Depositor

   Debra Hansen

   Article

   Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

   Inserts

   BB0323
   P13
   DipA
   Lmp1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   wild-type, full-length (signal-peptide-containing…

   Promoter

   Available Since

   May 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. lenti-SpCas9 puro

   Plasmid

   #104994

   Purpose

   This 3rd generation lentiviral construct delivers hSpCas9 and puromycin resistance.

   Depositor

   Brett Stringer

   Article

   Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

   Insert

   KpnI-XhoI-BsrGI-NheI
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. 6xHis-MBP-BrCas12b-RFND

   Plasmid

   #195339

   Purpose

   Expresses Engineered BrCas12b in Bacteria

   Depositor

   Piyush Jain

   Article

   Nguyen et al Cell Rep Med. 2023 May 16;4(5):101037. d

   Insert

   BrCas12b
   

   Use

   CRISPR and Synthetic Biology

   Tags

   6xHis and 6xHis, MBP, TEV cleavage site

   Expression

   Bacterial

   Mutation

   R160E, F208W, N524V, D868V

   Promoter

   T7

   Available Since

   May 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-P3-Alb-mScarlet

   Plasmid

   #183461

   Purpose

   To produce AAV with the P3 promoter to express Alb(mouse)-mScarlet fusion protein

   Depositor

   Hajime Hirase

   Article

   Wang et al Cell Rep Methods. 2022 Sep 21;2(10):1003

   Insert

   Alb (Alb Mouse)
   

   Use

   AAV

   Tags

   mScarlet

   Expression

   Mutation

   Promoter

   Available Since

   May 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-P3-Alb-mNG

    Plasmid

    #183460

    Purpose

    To produce AAV with the P3 promoter to express Alb(mouse)-mNeonGreen fusion protein

    Depositor

    Hajime Hirase

    Article

    Wang et al Cell Rep Methods. 2022 Sep 21;2(10):1003

    Insert

    Alb (Alb Mouse)
    

    Use

    AAV

    Tags

    mNeonGreen

    Expression

    Mutation

    Promoter

    Available Since

    May 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. 3X FLAG-FUS-WT

    Plasmid

    #44985

    Depositor

    Robin Reed

    Article

    Yamazaki et al Cell Rep. 2012 Oct 25;2(4):799-806. doi:

    Insert

    FUS (Full length) (FUS Human)
    

    Use

    Tags

    3X FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. Lenti-RNF2-ngRNA+41_EF1a-puroR

    Plasmid

    #207358

    Purpose

    Lentiviral transfer plasmid encoding hU6-driven expression of a RNF2 nicking guide RNA and EF1a-driven puromycin resistance gene.

    Depositor

    Marianne Carlon

    Article

    Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

    Insert

    RNF2 +41 ngRNA
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTRIP-SFFV-EGFP-NLS-FLAG-cGAS

    Plasmid

    #127659

    Purpose

    Over-express EGFP-NLS-FLAG-cGAS

    Depositor

    Nicolas Manel

    Article

    Gentili et al Cell Rep. 2019 Feb 26;26(9):2377-2393.e1

    Insert

    cGAS (CGAS Human)
    

    Use

    Lentiviral

    Tags

    EGFP-NLS-Flag

    Expression

    Mutation

    Promoter

    Available Since

    July 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. Phage-PGK-Flag-HA-FKBP-T(WT)

    Plasmid

    #181734

    Purpose

    for lentiviral expression of brachyury ORF in mammalian cells

    Depositor

    Charles Lin

    Article

    Sheppard et al Cell Rep Med. 2021 Jan 19;2(1):100188. d

    Insert

    T (brachyury)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    a silent mutation in the PAM site of an sgRNAt ta…

    Promoter

    Available Since

    March 21, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. GABA(A)R, Alpha1 scFv [N95/35]

    Plasmid

    #199430

    Purpose

    Mammalian expression of GABA(A)R, Alpha1 scFv. Derived from hybridoma N95/35.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    recombinant mouse scFv targetingGABA(A)R, Alpha1 (Human) (GABRA1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pX330-U6-Chimeric_BB-CBh-hSpCas9-hGem(1/110)

    Plasmid

    #71707

    Purpose

    A human codon-optimized SpCas9 fused to first 110 amino acids of human GEMININ and chimeric guide RNA expression plasmid

    Depositor

    Giulio Draetta

    Article

    Gutschner et al Cell Rep. 2016 Feb 3. pii: S2211-1247(16

    Insert

    humanized S. pyogenes Cas9 fused to human Geminin (GMNN Human)
    

    Use

    CRISPR

    Tags

    3xFLAG and hGEM(1/110)

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available Since

    Feb. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. VGAT scFv [L118/14]

    Plasmid

    #206754

    Purpose

    Mammalian Expression of VGAT scFV. Derived from hybridoma L118/14 scFv.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    VGAT (Mus musculus) recombinant scFV (Slc32a1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-CBh-Alb-mNG

    Plasmid

    #183466

    Purpose

    To produce AAV with the CBh promoter to express Alb(mouse)-mNeonGreen fusion protein

    Depositor

    Hajime Hirase

    Article

    Wang et al Cell Rep Methods. 2022 Sep 21;2(10):1003

    Insert

    Alb (Alb Mouse)
    

    Use

    AAV

    Tags

    mNeonGreen

    Expression

    Mutation

    Promoter

    Available Since

    May 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. GAD67 scFv [L127/8]

    Plasmid

    #190509

    Purpose

    Mammalian Expression of GAD67 scFV. Derived from hybridoma L127/8.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    GAD67 (Homo sapiens) recombinant scFV (GAD1 Mouse)
    

    Use

    Tags

    Sortase, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. LRP4 (extracellular) scFv [N207/27]

    Plasmid

    #190537

    Purpose

    Mammalian Expression of LRP4 (extracellular) scFV. Derived from hybridoma N207/27.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    LRP4 (extracellular) (Mus musculus) recombinant scFV (Lrp4 Mouse)
    

    Use

    Tags

    HA, Sortase, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. GFAP scFv [N206A/8]

    Plasmid

    #199426

    Purpose

    Mammalian expression of GFAP scFv. Derived from hybridoma N206A/8.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    recombinant mouse scFv targetingGFAP (Human) (GFAP Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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22. pcDNA3.1 SIRT7_H187Y

    Plasmid

    #53151

    Purpose

    expression vector for catalytically inactive human Sirtuin7

    Depositor

    Katrin Chua

    Article

    Shin et al Cell Rep. 2013 Nov 14;5(3):654-65. doi:

    Insert

    Sirt7 (SIRT7 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    H187Y, catalytic inactive

    Promoter

    CMV

    Available Since

    May 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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23. pTRIP-CMV-Puro-2A-NLS-FLAG-cGAS

    Plasmid

    #127656

    Purpose

    Over-express NLS-FLAG-cGAS

    Depositor

    Nicolas Manel

    Article

    Gentili et al Cell Rep. 2019 Feb 26;26(9):2377-2393.e1

    Insert

    cGAS (CGAS Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mutation

    Promoter

    Available Since

    July 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pNL-CEF-CD79B-RFP

    Plasmid

    #187005

    Purpose

    Expression of human CD79B as RFP fusion protein

    Depositor

    Hermann Eibel

    Article

    Sevdali et al Cell Rep. 2022 Jun 28;39(13):111019. doi

    Insert

    CD79B (CD79B Human)
    

    Use

    Lentiviral

    Tags

    tagRFP

    Expression

    Mutation

    Promoter

    Available Since

    July 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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25. pPGC1a_Proximal Promoter-FLuc2P

    Plasmid

    #154259

    Purpose

    Destabilized firefly luciferase reporter containing the Human PGC-1alpha Proximal Promoter from -500 to +52.

    Depositor

    Antonio Amelio

    Article

    Musicant et al Cell Rep. 2021 Feb 23;34(8):108768. doi:

    Insert

    PGC-1a Proximal Promoter (PPARGC1A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human PGC-1alpha Proximal Promoter

    Available Since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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26. pTRIP-SFFV-EGFP-FLAG-cGAS E225A/D227A

    Plasmid

    #127662

    Purpose

    Over-express catalytically inactive EGFP-FLAG-cGAS E225A/D227A

    Depositor

    Nicolas Manel

    Article

    Gentili et al Cell Rep. 2019 Feb 26;26(9):2377-2393.e1

    Insert

    cGAS (CGAS Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    E225A/D227A

    Promoter

    Available Since

    July 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Gephyrin scFv [L106/23]

    Plasmid

    #206775

    Purpose

    Mammalian Expression of Gephyrin scFV. Derived from hybridoma L106/23 scFv.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    Gephyrin (Homo sapiens) recombinant scFV (GPHN Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. N-Terminal Split Cas9 D10A Nickase with GyrA intein

    Plasmid

    #58695

    Purpose

    Expresses N-terminus of D10A SpCas9 nickase domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with C-Terminal Split Cas9 Gyra Intein for full length SpCas9 nickase production

    Depositor

    Gang Bao

    Article

    Fine et al Sci Rep. 2015 Jul 1;5:10777. doi: 10.103

    Insert

    D10A Nickase humanized S. pyogenes Cas9 with Gyra Nsplit Intein
    

    Use

    AAV and CRISPR

    Tags

    GyrA Nsplit Intein, HA Tag, and NLS

    Expression

    Mammalian

    Mutation

    D10A nickase converting mutation to SpCas9

    Promoter

    CBh

    Available Since

    Sept. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pTRIP-SFFV-EGFP-NLS-FLAG- cGAS E225A/D227A

    Plasmid

    #127660

    Purpose

    Over-express catalytically inactive EGFP-NLS-FLAG-cGAS E225A/D227A

    Depositor

    Nicolas Manel

    Article

    Gentili et al Cell Rep. 2019 Feb 26;26(9):2377-2393.e1

    Insert

    cGAS (CGAS Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    E225A/D227A

    Promoter

    Available Since

    July 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pPGC1a_Distal Promoter-FLuc2P

    Plasmid

    #154260

    Purpose

    Destabilized firefly luciferase reporter containing the upstream Human PGC-1alpha Distal Promoter from -500 to +52.

    Depositor

    Antonio Amelio

    Article

    Musicant et al Cell Rep. 2021 Feb 23;34(8):108768. doi:

    Insert

    PGC-1a Distal Promoter (PPARGC1A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human PGC-1alpha Distal Promoter

    Available Since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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