We narrowed to 8,311 results for: Set

1. Fast-GCaMP6f-RS09

   Plasmid

   #67160

   Purpose

   genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

   Depositor

   Samuel Wang

   Article

   Badura et al Neurophotonics. 2014 Oct;1(2):025008.

   Insert

   Fast-GCaMP6f-RS09
   

   Use

   Tags

   6xHis, T7 tag, and Xpress tag

   Expression

   Bacterial

   Mutation

   L414T within GCaMP6f

   Promoter

   T7

   Available since

   July 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNR4A1-donor

   Plasmid

   #112295

   Purpose

   CRISPR donor plasmid to tag human transcription factor NR4A1 with GFP

   Depositor

   Kevin White

   Insert

   NR4A1 homology arms flanking EGFP-IRES-Neo cassette (NR4A1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   hg38:12:52058840:C>T, hg38:12:52058930:A>C,…

   Promoter

   Available since

   Nov. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. FDP-P10B2-URA3

   Plasmid

   #130875

   Purpose

   Integration cassette for OrthoRep containing mKate driven by promoter 10B2

   Depositor

   Chang Liu

   Article

   Zhong et al ACS Synth Biol. 2018 Dec 21;7(12):2930-2

   Insert

   mKate
   

   Use

   Expression of mkate, leu2(q180*), and ura3 on ort…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. BASIC_SEVA_27_pKD46.10

   Plasmid

   #178922

   Purpose

   BASIC DNA assembly vector

   Depositor

   Paul Freemont

   Article

   Haines et al Synth Biol (Oxf). 2022 Oct 11;7(1):ysac0

   Insert

   mScarlet counter-selection cassette
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 7, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pYTK-DN1

   Plasmid

   #180282

   Purpose

   Cas9 cassette (TU1)

   Depositor

   Andreas Milias-Argeitis

   Article

   Novarina et al STAR Protoc. 2022 Jun 10;3(2):101358. do

   Insert

   ConLS - pPGK1 - Cas9 - tPGK1 - ConR1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pPGK1

   Available since

   June 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCCL-GKL1RC-Short-LEUg1mKateg2GKL2ORF10UCS

   Plasmid

   #52345

   Purpose

   Integration cassette to create p1-Short-Leu-mKate with p2 ORF10 UCS>mKate

   Depositor

   Chang Liu

   Article

   Ravikumar et al Nat Chem Biol. 2014 Mar;10(3):175-7. doi

   Insert

   mKate2
   

   Use

   P1 integration vector

   Tags

   Expression

   Bacterial

   Mutation

   changed V2H

   Promoter

   pGKL2 ORF10 UCS

   Available since

   June 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBigT

   Plasmid

   #21270

   Purpose

   Used for making transgenic mice. Contains a loxP-flanked cassette with a PGK-neo selectable marker and a tpA transcriptional stop sequence.

   Depositor

   Frank Costantini

   Article

   Srinivas et al BMC Dev Biol. 2001 . 1():4.

   Type

   Empty backbone

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 9, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCRISPR-Cas9-ScaligD

   Plasmid

   #125688

   Purpose

   Gene knockout/in for actinomycetes

   Depositor

   Tilmann Weber

   Article

   Tong et al ACS Synth Biol. 2015 Sep 18;4(9):1020-9.

   Insert

   streptomyces codon optimized spCas9, sgRNA casette, and ScaligD
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   ermE*/tipA

   Available since

   Nov. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDonor_Tn[sfGFP]

   Plasmid

   #213902

   Purpose

   Mariner transposon expressing sfGFP with kan selection casette

   Depositor

   James Collins

   Article

   English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

   Insert

   mariner transposon with kan resistance and superfolder GFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGGZ001

    Plasmid

    #48868

    Purpose

    Empty destination vector for creating a GreenGate plant transformation plasmid with the plant resistance cassette at the T-DNA right border.

    Depositor

    Jan Lohmann

    Article

    Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

    Type

    Empty backbone

    Use

    Golden gate compatible plant transformation vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTORGm43GWY

    Plasmid

    #112906

    Purpose

    Multisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to gentamicin. Contains additional cassette for constitutive expression of mCitrine.

    Depositor

    Sebastian Schornack

    Article

    Evangelisti et al BMC Microbiol. 2019 Nov 27;19(1):265. do

    Type

    Empty backbone

    Use

    Oomycete expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGGZ003

    Plasmid

    #48869

    Purpose

    Empty destination vector for creating a GreenGate plant transformation plasmid with the plant resistance cassette at the T-DNA left border.

    Depositor

    Jan Lohmann

    Article

    Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

    Type

    Empty backbone

    Use

    Golden gate compatible plant transformation vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pYPQ141B-ge4.1

    Plasmid

    #205144

    Purpose

    Guide RNA expression cassette harboring AtU3 promoter, ge4.1 5’ modification, and ge4.1 3’ modification

    Depositor

    Yiping Qi

    Article

    Tang et al Plant Genome. 2024 May 28:e20465. doi: 1

    Insert

    gRNA cloning site
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    AtU3

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMSCVII

    Plasmid

    #162750

    Purpose

    MSCV backbone

    Depositor

    Maki Nakayama

    Article

    Landry et al Bio Protoc. 2021 Jan 20;11(2):e3883. doi

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. DV4 VLPs pVRC8400

    Plasmid

    #63164

    Purpose

    DV4 prM-E structural cassette in pVRC8400

    Depositor

    Stephen Harrison

    Article

    Chao et al Elife. 2018 Jul 12;7. pii: 36461. doi: 1

    Insert

    DV4 prM-E
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. DV3 VLPs pVRC8400

    Plasmid

    #63163

    Purpose

    DV3 prM-E structural cassette in pVRC8400

    Depositor

    Stephen Harrison

    Article

    Chao et al Elife. 2018 Jul 12;7. pii: 36461. doi: 1

    Insert

    DV3 prM-E
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAV-Stuffer

    Plasmid

    #106248

    Purpose

    Destination vector for U6::gRNA expression cassette

    Depositor

    Charles Gersbach

    Article

    Thakore et al Nat Commun. 2018 Apr 26;9(1):1674. doi:

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJW1662

    Plasmid

    #112050

    Purpose

    C-terminal tag with - AID*(AtIAA17_71-114)-HA and OsTIR1 expression cassette marked with LEU

    Depositor

    Jonathan Weissman

    Article

    Costa et al Science. 2018 Jan 18. pii: science.aar36

    Inserts

    IAA17
    TIR1
    

    Use

    Tags

    HA

    Expression

    Yeast

    Mutation

    only amino acids 71-114

    Promoter

    ADH1 and None

    Available since

    Oct. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSLS.436

    Plasmid

    #184953

    Purpose

    Express -Eco1 RT and ncRNA

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1: ncRNA(wt) and RT
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTORGm43GWR

    Plasmid

    #112905

    Purpose

    Multisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to gentamicin. Contains additional cassette for constitutive expression of tdTomato.

    Depositor

    Sebastian Schornack

    Article

    Evangelisti et al BMC Microbiol. 2019 Nov 27;19(1):265. do

    Type

    Empty backbone

    Use

    Oomycete expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pPAP004

    Plasmid

    #160911

    Purpose

    E.coli/ P.pastoris shuttle plasmid for episomal expression in P.pastoris

    Depositor

    Sylvestre Marillonnet

    Article

    Pullmann et al ACS Synth Biol. 2021 Jun 2. doi: 10.1021

    Insert

    lacZ selection cassette
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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22. pSAC212

    Plasmid

    #187912

    Purpose

    psac212-crispey-bar-ade2KO

    Depositor

    Hunter Fraser

    Article

    Chen et al Cell Genom. 2023 Feb 28;3(4):100273. doi

    Insert

    ade2 KO CRISPEY cassette
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pTORGm43GWC

    Plasmid

    #112903

    Purpose

    Multisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to gentamicin. Contains additional cassette for constitutive expression of mTFP1.

    Depositor

    Sebastian Schornack

    Article

    Evangelisti et al BMC Microbiol. 2019 Nov 27;19(1):265. do

    Type

    Empty backbone

    Use

    Oomycete expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pTORGm43GWF

    Plasmid

    #112904

    Purpose

    Multisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to gentamicin. Contains additional cassette for constitutive expression of mWasabi.

    Depositor

    Sebastian Schornack

    Article

    Evangelisti et al BMC Microbiol. 2019 Nov 27;19(1):265. do

    Type

    Empty backbone

    Use

    Oomycete expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAct-GW-HygroR

    Plasmid

    #149610

    Purpose

    Gateway compatible expression plasmid for Drosophila and establishing stable cell lines by Hygromycin selection. Actin5c ubiquitous promoter.

    Depositor

    Norbert Perrimon

    Article

    Bosch et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

    Insert

    Gateway cassette (ccdb + Chlor.R.)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Act5c

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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26. pSWAP_Entry_H2_beta_CM

    Plasmid

    #184870

    Purpose

    pSwap plasmid part with H2_beta homology arms and chloramphenicol cassette

    Depositor

    Patrick Sobetzko

    Article

    Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

    Insert

    lacZalpha,ccdB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 2, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

27. BASIC_SEVA_17_pKD46.10

    Plasmid

    #178917

    Purpose

    BASIC DNA assembly vector

    Depositor

    Paul Freemont

    Article

    Haines et al Synth Biol (Oxf). 2022 Oct 11;7(1):ysac0

    Insert

    mScarlet counter-selection cassette
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 2, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

28. pBS-KS-attB1-2-PT-SA-SD-0-2xTY1-V5

    Plasmid

    #61255

    Purpose

    CRISPR-RMCE, second step attB plasmid, intron phase 0, exchange dsRed with 2xTY1 and V5 tags cassette

    Depositor

    Frank Schnorrer

    Article

    Zhang et al G3 (Bethesda). 2014 Oct 15. pii: g3.114.

    Insert

    2xTY1-V5
    

    Use

    CRISPR

    Tags

    TY1 and V5

    Expression

    Mutation

    Promoter

    Available since

    Nov. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pRE57-Ter

    Plasmid

    #170635

    Purpose

    DNA donor for transposition

    Depositor

    Sheng Yang

    Article

    Zhang et al CRISPR J. 2021 Jun;4(3):350-359. doi: 10

    Insert

    Mini-Tn6677, Ter cassette
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pE_Cre_lambdaCos

    Plasmid

    #173785

    Purpose

    The pE_Cre_lambdaCos plasmid is used to remove the antibiotic resistance cassette from pIT5 integrants.

    Depositor

    Keith Shearwin

    Article

    Hao et al ACS Synth Biol. 2021 Jun 30. doi: 10.102

    Insert

    Cre
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    Expression

    Mutation

    S102Y and S279Y- please see depositor comments

    Promoter

    Available since

    Dec. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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