Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 10,761 results for: T7

Clear keywords
Showing: 3741 - 3760 of 10761 results

  2. pJK290

    Plasmid
    #71554
    Purpose
    Production of Acetobacter aceti 1023 alanine racemase (alr) with double mutant (AaAlr-K39A,Y260A)
    Depositor
    T. J. Kappock
    Article
    Francois et al Protein Expr Purif. 2007 Jan;51(1):39-48
    Insert
    alanine racemase
    Expression
    Bacterial
    Mutation
    changes lysine-39 to alanine and tyrosine-260 to …
    Promoter
    T7
    Available Since
    March 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    71554-plasmid-map-sequence-id-139946

    Enlarge

  3. pJK598

    Plasmid
    #72523
    Purpose
    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase subunits, one with C-terminal His6 tag, and associated hypothetical protein (AaPurS, AaOrfY, AaPurQ, AaPurL)
    Depositor
    T. J. Kappock
    Inserts
    formylglycinamidine ribonucleotide synthetase, small subunit
    hypothetical protein in purSQL operon
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    synthetic gene
    Promoter
    T7 and n/a
    Available Since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    72523-plasmid-map-sequence-id-132608

    Enlarge

  4. pJK326

    Plasmid
    #71557
    Purpose
    Production of Acetobacter aceti 1023 alanine racemase (alr) with N-terminal His tag and Ala368>Asp mutant (H6AaAlr-A368D)
    Depositor
    T. J. Kappock
    Article
    Francois et al Protein Expr Purif. 2007 Jan;51(1):39-48
    Insert
    alanine racemase
    Tags
    His6
    Expression
    Bacterial
    Mutation
    changes alanine-368 to aspartate
    Promoter
    T7
    Available Since
    Feb. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    71557-plasmid-map-sequence-id-134622

    Enlarge

  5. pJK325

    Plasmid
    #71556
    Purpose
    Production of Acetobacter aceti 1023 alanine racemase (alr) with N-terminal His tag and Ala367>Asp mutant (H6AaAlr-A367D)
    Depositor
    T. J. Kappock
    Article
    Francois et al Protein Expr Purif. 2007 Jan;51(1):39-48
    Insert
    alanine racemase
    Tags
    His6
    Expression
    Bacterial
    Mutation
    changes alanine-367 to aspartate
    Promoter
    T7
    Available Since
    Feb. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    71556-plasmid-map-sequence-id-131415

    Enlarge

  6. pJK345

    Plasmid
    #71559
    Purpose
    Production of Acetobacter aceti 1023 alanine racemase (alr) with N-terminal His tag and Ala367/Ala368 deletion (H6AaAlr-A367del,A368del)
    Depositor
    T. J. Kappock
    Article
    Francois et al Protein Expr Purif. 2007 Jan;51(1):39-48
    Insert
    alanine racemase
    Tags
    His6
    Expression
    Bacterial
    Mutation
    deletes alanine-367 and alanine-368
    Promoter
    T7
    Available Since
    Feb. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    71559-plasmid-map-sequence-id-134626

    Enlarge

  7. pJK344

    Plasmid
    #71558
    Purpose
    Production of Acetobacter aceti 1023 alanine racemase (alr) with N-terminal His tag and Ala368 deletion (H6AaAlr-A368del)
    Depositor
    T. J. Kappock
    Article
    Francois et al Protein Expr Purif. 2007 Jan;51(1):39-48
    Insert
    alanine racemase
    Tags
    His6
    Expression
    Bacterial
    Mutation
    deletes alanine-367 (same as deleting alanine-368)
    Promoter
    T7
    Available Since
    Feb. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    71558-plasmid-map-sequence-id-134625

    Enlarge

  8. pJK612

    Plasmid
    #72533
    Purpose
    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase subunits; associated hypothetical protein N-terminal domain (AaPurS, AaOrfY[1-69], AaPurQ, AaPurL)
    Depositor
    T. J. Kappock
    Inserts
    formylglycinamidine ribonucleotide synthetase, small subunit
    hypothetical protein in purSQL operon
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    Expression
    Bacterial
    Mutation
    residues 1-69 only; C-terminal domain removed and…
    Promoter
    T7 and n/a
    Available Since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72533-plasmid-map-sequence-id-132621

    Enlarge

  9. pJK619

    Plasmid
    #72536
    Purpose
    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit, N-term His-tag; hypothet. protein; glutaminase subunit; large subunit (H6AaPurS, AaOrfY, AaPurQ, AaPurL)
    Depositor
    T. J. Kappock
    Inserts
    formylglycinamidine ribonucleotide synthetase, small subunit
    hypothetical protein in purSQL operon
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    Tags
    His6
    Expression
    Bacterial
    Mutation
    synthetic gene
    Promoter
    T7 and n/a
    Available Since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72536-plasmid-map-sequence-id-132627

    Enlarge

  11. pST50Trc4-HPCNDHFR

    Plasmid
    #63998
    Purpose
    Position 4 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Tags
    Protein C (HPC); TEV cleavable
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Dec. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    63998-plasmid-map-sequence-id-177404

    Enlarge

  12. pST50Trc4-CBPNDHFR

    Plasmid
    #63995
    Purpose
    Position 4 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Tags
    Calmodulin Binding Peptide (CBP); TEV cleavable
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Dec. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    63995-plasmid-map-sequence-id-177401

    Enlarge

  13. pST50Trc4-HPCDHFR

    Plasmid
    #63993
    Purpose
    Position 4 transfer plasmid for pST44 polycistronic plasmid suite; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Tags
    Protein C (HPC)
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Dec. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    63993-plasmid-map-sequence-id-177399

    Enlarge

  14. pST50Trc3-DHFRCYD

    Plasmid
    #63988
    Purpose
    Position 3 transfer plasmid for pST44 polycistronic plasmid suite; C-term single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Tags
    Covalent Yet Dissociable (CYD) [AGTEFCA]
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Dec. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    63988-plasmid-map-sequence-id-177394

    Enlarge

  15. pST50Trc3-HPCDHFR

    Plasmid
    #63975
    Purpose
    Position 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Tags
    Protein C (HPC)
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Dec. 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    63975-plasmid-map-sequence-id-177381

    Enlarge

  16. pST50Trc3-HPCHISNDHFR

    Plasmid
    #63984
    Purpose
    Position 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Tags
    6xHis & Protein C (HPC); TEV cleavable
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Dec. 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    63984-plasmid-map-sequence-id-177534

    Enlarge

  17. pST50Trc2-HPCNDHFR

    Plasmid
    #63962
    Purpose
    Position 2 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Tags
    Protein C (HPC); TEV cleavable
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Dec. 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    63962-plasmid-map-sequence-id-177368

    Enlarge

  18. pST50Trc3-FLAGDHFR

    Plasmid
    #63974
    Purpose
    Position 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Tags
    FLAG
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Dec. 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    63974-plasmid-map-sequence-id-177380

    Enlarge

  19. pST50Trc1-HPCHISNDHFR

    Plasmid
    #63948
    Purpose
    Position 1 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Tags
    6xHis & Protein C (HPC); TEV cleavable
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Dec. 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    63948-plasmid-map-sequence-id-177464

    Enlarge

  20. pST50Trc1-CBPHISNDHFR

    Plasmid
    #63945
    Purpose
    Position 1 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Tags
    6xHis & Calmodulin Binding Peptide (CBP); TEV…
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Dec. 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    63945-plasmid-map-sequence-id-177351

    Enlarge

Showing: 3741 - 3760 of 10761 results