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pDmREDusk

Plasmid

#213701

Purpose

Vector for red-light-repressed bacterial gene expression

Depositor

Andreas Moeglich

Publication

Meier et al Nat Commun. 2024 Jun 10;15(1):4876. doi:

Type

Empty backbone

Expression

Bacterial

Available since

May 30, 2024

Availability

Academic Institutions and Nonprofits only

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lenti-fullef1a-FAKSPARK2

Plasmid

#195265

Purpose

express wtFAKSPARK2 in a lenti vector

Depositor

Xiaokun Shu

Publication

Li et al Nat Chem Biol. 2023 Jun 22. doi: 10.1038

Insert

FAKSPARK2 sensor

Use

Lentiviral

Expression

Mammalian

Promoter

ef1a

Available since

Nov. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pYZ462

Plasmid

#187970

Purpose

TEF1p-Cas9-CYC1t and SNR52p-Not1-SUP4t

Depositor

Jef Boeke

Publication

Zhao et al Nucleic Acids Res. 2023 Jun 16:gkad491.

Insert

Cas9 (codon optimized for human)

Use

Synthetic Biology

Expression

Yeast

Promoter

TEF1

Available since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pSWAP_Entry_HAIB

Plasmid

#184869

Purpose

For scarless integration into the chromosome, HAIB plasmid can be usedin place of the HA, INS and HB modules.

Depositor

Patrick Sobetzko

Publication

Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

Insert

lacZalpha,ccdB

Expression

Bacterial

Available since

Jan. 3, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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CAG.3xHalo-GFP-SPC25

Plasmid

#176206

Purpose

Kinetochore anchor for TMP-Halo dimerization system

Depositor

Michael Lampson

Publication

Chen et al J Cell Biol. 2021 Jun 7;220(6). pii: 212

Insert

3xHalo-GFP-SPC25

Expression

Mammalian

Available since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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His-GFP-Granuphilin

Plasmid

#170867

Purpose

Mammalian expression of His-GFP-Granuphilin.

Depositor

Justin Taraska

Publication

Prasai et al Nat Commun. 2021 Jun 25;12(1):3970. doi:

Insert

Synaptotagmin-like 4 (SYTL4 Human)

Tags

(His)6 and EGFP

Expression

Mammalian

Promoter

CMV

Available since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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pRMCD28

Plasmid

#167556

Purpose

Plasmid pWSK29 encoding E. coli PhoA' for making reporter fusion proteins for topology mapping

Depositor

Renato Morona

Publication

Daniels et al Mol Microbiol. 1998 Jun;28(6):1211-22. d

Type

Empty backbone

Tags

PhoA'

Expression

Bacterial

Available since

May 18, 2021

Availability

Academic Institutions and Nonprofits only

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pQdCas9-sgempty

Plasmid

#159523

Purpose

Expression of dCas9 driven by lux cassette from Vibrio fischeri

Depositor

Radhakrishnan Mahadevan

Publication

Liu et al ACS Synth Biol. 2020 Jun 5. doi: 10.1021

Insert

quorum sensing cassette luxI, luxR, and the LuxR-dependent promoter pLuxI , dcas9 and sgRNA

Use

CRISPR

Promoter

quorum sensing promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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EGFP-UTRN-mini

Plasmid

#158749

Purpose

Expresses a shorter version of Utrophin (amino acid 35-136) with amino terminus eGFP in mammalian cells

Depositor

Minhajuddin Sirajuddin

Publication

Kumari et al EMBO J. 2020 Jun 22:e104006. doi: 10.152

Insert

UTRN-mini

Tags

eGFP

Expression

Mammalian

Promoter

CMV IE94

Available since

Sept. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pARR5::LUC

Plasmid

#141170

Purpose

Luminescent reporter for cytokinin signaling

Depositor

Vardis Ntoukakis, Patrick Schäfer

Publication

Lehmann et al PLoS One. 2020 Jun 4;15(6):e0234154. doi

Insert

AtARR5 promoter

Use

Luciferase

Expression

Plant

Promoter

AtARR5, AT3G48100

Available since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pJF104B

Plasmid

#113324

Purpose

pTet-dCas9, pTet-MCP-SoxS_R93A

Depositor

Jesse Zalatan

Publication

Dong et al Nat Commun. 2018 Jun 27;9(1):2489. doi:

Insert

dCas9 and MCP-SoxS_R93A

Use

CRISPR

Expression

Bacterial

Mutation

R93A

Available since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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Dppa1-T2A-H2B-Halo donor

Plasmid

#113118

Purpose

Donor plasmid for knock-in T2A-H2B-Halo to the c-terminal of mouse Dppa1 coding sequence

Depositor

Janet Rossant

Publication

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

Dppa1 donor region including homology arms and T2A-H2B-Halo

Use

Synthetic Biology

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pRJPaph-gusA

Plasmid

#67036

Purpose

Plasmid for Paph-gusA integration downstream of B. diazoefficiens (B. japonicum) scoI

Depositor

Julia Vorholt

Publication

Ledermann et al Mol Plant Microbe Interact. 2015 Jun 2.

Inserts

B. diazoefficiens DNA from scoI downstream region (comprising blr1132')
gusA

Use

Conjugative bacterial vector

Available since

Oct. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pCDNAIIIB cdc42 QL

Plasmid

#61636

Purpose

mammalian expression of cdc42 constitutively active mutant

Depositor

JS Gutkind

Publication

Coso et al Cell. 1995 Jun 30;81(7):1137-46.

Insert

Cdc42 (CDC42 Human)

Expression

Mammalian

Mutation

Q61L, constitutively active

Promoter

CMV

Available since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
pBp-FGFR2b-K660E

Plasmid

#45705

Depositor

Matthew Meyerson

Publication

Liao et al Cancer Res. 2013 Jun 20.

Insert

FGFR2 IIIb (FGFR2 Human)

Use

Retroviral

Expression

Mammalian

Mutation

K660E

Available since

June 24, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA-HA-hTRAF3 333-C

Plasmid

#44035

Depositor

Shao-Cong Sun

Publication

Liao et al J Biol Chem. 2004 Jun 18;279(25):26243-5

Insert

TNF receptor-associated factor 3 (TRAF3 Human)

Tags

1xHA

Expression

Mammalian

Mutation

Contains amino acids 334-568; S393G and K492N

Available since

April 4, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pRK5 Flag Grb10 5A short

Plasmid

#37484

Depositor

David Sabatini

Publication

Hsu et al Science. 2011 Jun 10;332(6035):1317-22.

Insert

Grb10 5A (GRB10 Human)

Tags

Flag

Expression

Mammalian

Mutation

isoform C (missing N-term 58aa), 536aa, S150A T15…

Available since

July 23, 2012

Availability

Academic Institutions and Nonprofits only
pT5T_hp53_ST_CR2

Plasmid

#34923

Depositor

Thanos Halazonetis

Publication

Petty et al EMBO J. 2011 Jun 1;30(11):2167-76. Epub

Insert

p53 DNA-binding (Res 94-358, deletion 293-321) (TP53 Human)

Expression

Bacterial

Mutation

C135V, C141V, W146Y, C182S, V203A, R209P, C229Y, …

Promoter

T7

Available since

Feb. 22, 2012

Availability

Academic Institutions and Nonprofits only

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pENTR APLP2 shRNA 2

Plasmid

#30160

Depositor

Dennis Selkoe, Tracy Young-Pearse

Publication

Young-Pearse et al Neural Dev. 2008 Jun 23;3:15. doi: 10.11

Insert

APLP2 shRNA 2

Expression

Mammalian

Available since

July 28, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pCS2+ cas

Plasmid

#26729

Depositor

Didier Stainier

Publication

Kikuchi et al Genes Dev. 2001 Jun 15. 15(12):1493-505.

Insert

casanova (sox32 Zebrafish)

Use

Xenopus/mammalian/avian/zebrafish

Available since

Jan. 18, 2011

Availability

Academic Institutions and Nonprofits only