We narrowed to 14,039 results for: TIM

1. pENTR-TC9

   Plasmid

   #202088

   Purpose

   entry vector for gateway recombination of TevCas9 and sgRNA into a destination cassette in pCitro-dest

   Depositor

   David Edgell

   Article

   Hamilton et al ACS Synth Biol. 2023 Dec 15;12(12):3578-

   Insert

   TevCas9 + sgRNA cassette
   

   Use

   CRISPR

   Available Since

   June 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pVAX1-VSVG-INDIANA2

   Plasmid

   #196623

   Purpose

   Express VSV-G protein in mammalian cells.

   Depositor

   Nicolas Manel

   Article

   Jneid et al Sci Immunol. 2023 Jan 13;8(79):eabn6612.

   Insert

   VSV-G
   

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFR-mTq2-antiFRET-P2A-RCaMP1h

   Plasmid

   #191467

   Purpose

   Compare the intensity of the genetically encoded calcium sensor (GECI) RCaMP1h to mTurquoise2 fluorescent protein

   Depositor

   Dorus Gadella

   Article

   van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

   Insert

   RCaMP1h
   

   Tags

   His-mTurquoise2-antiFRETlinker-P2A

   Expression

   Bacterial and Mammalian

   Available Since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. OA-1163A

   Plasmid

   #194001

   Purpose

   pBac-AedesUbiq-RfxCas13d (Codon optimized)-p10-Opie2-dsRed.

   Depositor

   Omar Akbari

   Article

   Dalla Benetta et al CRISPR J. 2023 Dec;6(6):543-556. doi: 10

   Insert

   Uniq-CasRx (Codon optimized)
   

   Expression

   Insect

   Available Since

   Jan. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFR-mScarletI-antiFRET-P2A-GCaMP3

   Plasmid

   #191460

   Purpose

   Compare the intensity of the genetically encoded calcium sensor (GECI) GCaMP3 to mScarlet-I fluorescent protein

   Depositor

   Dorus Gadella

   Article

   van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

   Insert

   GCaMP3
   

   Tags

   His-mScarletI-antiFRETlinker-P2A

   Expression

   Bacterial and Mammalian

   Available Since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFR-mTq2-antiFRET-P2A-K-GECO1

   Plasmid

   #191469

   Purpose

   Compare the intensity of the genetically encoded calcium sensor (GECI) K-GECO1 to mTurquoise2 fluorescent protein

   Depositor

   Dorus Gadella

   Article

   van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

   Insert

   K-GECO1
   

   Tags

   His-mTurquoise2-antiFRETlinker-P2A

   Expression

   Bacterial and Mammalian

   Available Since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFR-mTq2-antiFRET-P2A-R-GECO1

   Plasmid

   #191471

   Purpose

   Compare the intensity of the genetically encoded calcium sensor (GECI) R-GECO1 to mTurquoise2 fluorescent protein

   Depositor

   Dorus Gadella

   Article

   van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

   Insert

   R-GECO1
   

   Tags

   His-mTurquoise2-antiFRETlinker-P2A

   Expression

   Bacterial and Mammalian

   Available Since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA-RCC

   Plasmid

   #190635

   Purpose

   Mammalian expression of genetically encoded calcium indicator RCC

   Depositor

   Robert Campbell

   Article

   Gohil et al ACS Sens. 2023 Feb 24;8(2):587-597. doi:

   Insert

   RCC
   

   Expression

   Mammalian

   Available Since

   Nov. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDRESS-mScarletI-antiFRET-P2A2-linker_noSacI

   Plasmid

   #191473

   Purpose

   Negative control for ratiometric comparison between green fluorescent proteins and sensors

   Depositor

   Dorus Gadella

   Article

   van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

   Insert

   none
   

   Tags

   His-mScarletI-antiFRETlinker-P2A

   Expression

   Bacterial and Mammalian

   Available Since

   Nov. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDRESS-mTq2-antiFRET-P2A2-linker_noSacI

    Plasmid

    #191472

    Purpose

    Negative control for ratiometric comparison between red fluorescent proteins and sensors

    Depositor

    Dorus Gadella

    Article

    van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

    Insert

    none
    

    Tags

    His-mTurquoise2-antiFRETlinker-P2A

    Expression

    Bacterial and Mammalian

    Available Since

    Nov. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFR-mTq2-antiFRET-P2A-CH-GECO2.1

    Plasmid

    #191470

    Purpose

    Compare the intensity of the genetically encoded calcium sensor (GECI) CH-GECO2.1 to mTurquoise2 fluorescent protein

    Depositor

    Dorus Gadella

    Article

    van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

    Insert

    CH-GECO2.1
    

    Tags

    His-mTurquoise2-antiFRETlinker-P2A

    Expression

    Bacterial and Mammalian

    Available Since

    Nov. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFR-mScarletI-antiFRET-P2A-jGCaMP7c

    Plasmid

    #191461

    Purpose

    Compare the intensity of the genetically encoded calcium sensor (GECI) jGCaMP7c to mScarlet-I fluorescent protein

    Depositor

    Dorus Gadella

    Article

    van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

    Insert

    jGCaMP7c
    

    Tags

    His-mScarletI-antiFRETlinker-P2A

    Expression

    Bacterial and Mammalian

    Available Since

    Nov. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA5/FRT/TO_H2B-STf

    Plasmid

    #181998

    Purpose

    CMV overexpression of SNAP-tag fast fusion to H2B for nuclear localization in mammalian cell lines

    Depositor

    Kai Johnsson

    Article

    Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

    Insert

    H2B-STf
    

    Expression

    Mammalian

    Mutation

    Fast mutation on SNAP-tag E30R

    Promoter

    CMV

    Available Since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDNA-SCcpV

    Plasmid

    #190636

    Purpose

    Mammalian expression of genetically encoded calcium indicator SCcpV

    Depositor

    Robert Campbell

    Article

    Gohil et al ACS Sens. 2023 Feb 24;8(2):587-597. doi:

    Insert

    SCcpV
    

    Expression

    Mammalian

    Available Since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCRISPR2_IHF_Opt

    Plasmid

    #149395

    Purpose

    P. aeruginosa PA14 CRISPR2 locus, with "optimized" IHFprox site

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

    Insert

    Pseudomonas aeruginosa PA14 CRISPR2 locus
    

    Expression

    Bacterial

    Mutation

    IHFprox site is optimized at key positions needed…

    Promoter

    pBAD

    Available Since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTrichoGate-14

    Plasmid

    #160714

    Purpose

    For C-terminal tags with Trichoderma optimised Synthetic 3X-c-Myc tag peptide (EQKLISEEDL) bordered by BsaI restriction sites

    Depositor

    Artemio Mendoza

    Article

    Nogueira-Lopez et al Front Microbiol. 2019 Dec 10;10:2794. do

    Insert

    Trichoderma optimised Synthetic 3X-c-Myc tag peptide (EQKLISEEDL)
    

    Use

    Synthetic Biology

    Available Since

    March 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCS2-zfCSF3b

    Plasmid

    #168202

    Purpose

    Express zebrafish Csf3b, mRNA synthesis

    Depositor

    Petr Bartunek

    Article

    Stachura et al Blood. 2013 Dec 5;122(24):3918-28. doi:

    Insert

    Csf3b (csf3b Zebrafish)
    

    Use

    Vertebrate expression (zebrafish, chicken, xenopu…

    Expression

    Mammalian

    Available Since

    Jan. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pN1mcherry-NKD2S31A

    Plasmid

    #174332

    Purpose

    encodes human NKD2 S31A mutant cDNA with a C-terminal mCherry fusion.

    Depositor

    Sonal Srikanth

    Article

    Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

    Insert

    Homo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)
    

    Tags

    mCherry tag

    Expression

    Mammalian

    Mutation

    changed Serine 31 to Alanine

    Promoter

    CMV

    Available Since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. H2A-mTurquoise-RhoJQ79L-deltaCaax

    Plasmid

    #176110

    Purpose

    Constitutively active, nucelar localized RhoJ

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

    Insert

    RhoJ (RHOJ Human)
    

    Expression

    Mammalian

    Mutation

    Q79L

    Available Since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCDNA3.1-NKD2S31A

    Plasmid

    #174331

    Purpose

    encodes human NKD2 S31A mutant cDNA with a C-terminal MycHis Tag.

    Depositor

    Sonal Srikanth

    Article

    Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

    Insert

    Homo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)
    

    Tags

    MycHis

    Expression

    Mammalian

    Mutation

    changed Serine 31 to Alanine

    Promoter

    CMV

    Available Since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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