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We narrowed to 5,870 results for: actin

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Showing: 3761 - 3780 of 5870 results

  2. pCAGGS-N-TEV-HAT

    Plasmid
    #202598
    Purpose
    Express C-terminal Tobacco Etch Virus (TEV) protease cleavable Histidine Affinity Tag (HAT) (KDHLIHNVHKEEHAHAHNK) in mammalian cell
    Depositor
    Chiaki Murakami
    Publication
    Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002
    Type
    Empty backbone
    Tags
    TEV cleavable site (ENLYFQG) and poly-histidine a…
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    July 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    202598-plasmid-map-sequence-id-399468

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  4. pCAG-DIO-inv[roxSTOProx-EB3 mNeonGreen-bGH]

    Plasmid
    #196885
    Purpose
    Dual Cre/Dre-dependent expression of EB3-mNeon. Used as functional (labelling of microtubule plus ends) Cre-Dre cotransfection reporter
    Depositor
    Frank Bradke
    Publication
    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16
    Insert
    roxSTOProx-EB3 mNeonGreen-bGH (MAPRE3 Human)
    Use
    Cre/Lox; Dre/rox
    Expression
    Mammalian
    Promoter
    Quimeric CAG
    Available since
    May 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    196885-plasmid-map-sequence-id-391918

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  5. pCAG-Halo-Tag-128-425-Akap9

    Plasmid
    #196873
    Purpose
    Expression of fragment 128-425 from A-Kinase Anchoring Protein 9 (Akap9) fused to Halo-Tag. Used to displace endogenous Akap9 from Golgi
    Depositor
    Frank Bradke
    Publication
    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Halo-Tag-Akap9 (128-425) (Akap9 Rat)
    Expression
    Mammalian
    Promoter
    Quimeric CAG
    Available since
    May 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    196873-plasmid-map-sequence-id-390445

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  6. AV15_pCAG.Cas9.gRNA.S1

    Plasmid
    #199259
    Purpose
    Expression construct encoding SpCas9 nuclease and an AAVS1-targeting guide RNA
    Depositor
    Manuel Goncalves
    Publication
    Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    SpCas9 nuclease
    AAVS1-targeting gRNA
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Mammalian
    Promoter
    CAG promoter and RNA polymerase III promoter for …
    Available since
    April 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    199259-plasmid-map-sequence-id-391662

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  7. AV44_pCAG.Cas9-D10A.gRNA.S1

    Plasmid
    #199258
    Purpose
    Expression construct encoding SpCas9-D10A nickase and an AAVS1-targeting guide RNA
    Depositor
    Manuel Goncalves
    Publication
    Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    SpCas9-D10A nickase
    AAVS1-targeting gRNA
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    CAG promoter and RNA polymerase III promoter for …
    Available since
    April 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    199258-plasmid-map-sequence-id-391654

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  10. pRV-TdTom-MCS

    Plasmid
    #193711
    Purpose
    Retroviral plasmid DNA for subcloning an oligonucleotide library encoding RNA barcodes
    Depositor
    Colin Akerman
    Type
    Empty backbone
    Use
    Retroviral
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    March 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    193711-plasmid-map-sequence-id-387442

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  11. AAV-CAG-dDIO-lck-smMyc-4x6T

    Plasmid
    #196421
    Purpose
    Dre-dependent AAV expression of membrane-targeted Myc spaghetti monster reporter preferentially in astrocytes; astrocyte selectivity generated with 4x6T miRNA targeting cassette
    Depositor
    Stanley Thomas Carmichael
    Publication
    Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:
    Insert
    Lck-smMyc
    Use
    AAV; Dre/rox; astrocyte-selective
    Tags
    Lck
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Feb. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    196421-plasmid-map-sequence-id-385348

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  15. AAVS1-Puro CAG IL13-CAR

    Plasmid
    #157742
    Purpose
    knock IL13-CAR into AAVS1 safe harbor locus for constitutive expression
    Depositor
    Xiaoping Bao
    Publication
    Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Chimeric antigen receptor targeting IL13
    Use
    CRISPR, Synthetic Biology, and TALEN
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    157742-plasmid-map-sequence-id-308792

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  16. pAAV2-CAG-mScarlet-I-Cdt1

    Plasmid
    #191100
    Purpose
    To express a bright monomeric red FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methods
    Depositor
    Murray Blackmore, Pantelis Tsoulfas
    Publication
    Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:
    Insert
    mScarlet-I-Cdt1 (30-120)
    Use
    AAV and Synthetic Biology
    Tags
    mScarlet-I fused to residues 30-120 of human Cdt1…
    Expression
    Mammalian
    Mutation
    Optimized to human codon usage
    Promoter
    CMV enhancer and CAG
    Available since
    Dec. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    191100-plasmid-map-sequence-id-380122

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  19. AAV-CAG-FLEX-NES-jGCaMP8m-WPRE

    Plasmid
    #186045
    Purpose
    AAV-mediated cytosolic expression of jGCaMP8m under the CAG promoter, Cre-dependent expression
    Depositor
    Loren Looger
    Insert
    NES-jGCaMP8m
    Use
    AAV
    Tags
    NES-6xHis
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Aug. 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    186045-plasmid-map-sequence-id-370173

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  20. AAV-CAG-FLEX-NES-jGCaMP8s-WPRE

    Plasmid
    #186046
    Purpose
    AAV-mediated cytosolic expression of jGCaMP8s under the CAG promoter, Cre-dependent expression
    Depositor
    Loren Looger
    Insert
    NES-jGCaMP8s
    Use
    AAV
    Tags
    NES-6xHis
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Aug. 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    186046-plasmid-map-sequence-id-370170

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Showing: 3761 - 3780 of 5870 results