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pUAS myc Gr66a

Plasmid

#26861

Depositor

Craig Montell

Article

Moon et al Curr Biol. 2006 Sep 19. 16(18):1812-7.

Insert

Gr66 gustatory receptor (Gr66a Fly)

Tags

myc

Expression

Insect

Available Since

July 11, 2011

Availability

Academic Institutions and Nonprofits only
pUAS-kkv-R896K::NG

Plasmid

#138957

Purpose

Used to generate a transgene that will express an inactive fluorescent Chitin Synthase in Drosophila using Gal4

Depositor

Paul Adler

Article

Adler et al bioRxiv 2020

Insert

kkv-R896K::NG (kkv Fly)

Tags

Neon Green fused to C terminus of Kkv

Expression

Insect

Mutation

R896K

Promoter

UAS

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pFOSGE_COF4A02

Plasmid

#71021

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Ube3a (Ube3a Fly)

Use

Gateway Cloning

Available Since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only
KHBD00233

Plasmid

#34377

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG7803 (z Fly)

Use

Gateway Cloning

Available Since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
pIS1

Plasmid

#74887

Purpose

targeting vector for C-terminal eGFP tag on Drosophila melanogaster Act5C

Depositor

Klaus Foerstemann

Article

Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

Inserts

Act5C upstream homology region
Act5C downstream homology

Tags

eGFP

Expression

Insect

Available Since

April 28, 2016

Availability

Academic Institutions and Nonprofits only

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pT7-MS2-HA-C1-MBP-DmBam_13-36_AG

Plasmid

#148839

Purpose

Mammalian Expression of DmBam_13-36

Depositor

Elisa Izaurralde

Article

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

DmBam_13-36 (bam Fly)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pFastBac FPC

Plasmid

#1927

Depositor

Robert Kingston

Article

Francis et al Mol Cell 2001 Sep;8(3):545-56.

Insert

Polycomb (Pc Fly)

Tags

flag

Expression

Insect

Available Since

March 23, 2006

Availability

Academic Institutions and Nonprofits only
orthodenticle

Plasmid

#37285

Depositor

Norbert Perrimon

Article

Finkelstein et al Genes Dev. 1990 Sep;4(9):1516-27.

Insert

orthodenticle (oc Fly)

Expression

Bacterial

Available Since

July 16, 2012

Availability

Academic Institutions and Nonprofits only
HS-H3.3A-GFP

Plasmid

#8696

Depositor

Kami Ahmad

Article

Ahmad et al Mol Cell 2002 Jun;9(6):1191-200.

Insert

H3.3A (His3.3A Fly)

Tags

GFP

Expression

Insect

Available Since

Aug. 15, 2005

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) Laccase2 MCS-HIPK3 Exon

Plasmid

#69899

Purpose

Expresses the human HIPK3 exon 2 circular RNA in mammalian cells

Depositor

Jeremy Wilusz

Article

Kramer et al Genes Dev. 2015 Oct 8.

Insert

HIPK3 (stw Fly, Human)

Expression

Mammalian

Mutation

Expresses aa1-194 of HIPK3

Promoter

CMV

Available Since

Nov. 11, 2015

Availability

Academic Institutions and Nonprofits only

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Hy_pMT Flag-Hel25E WT

Plasmid

#110123

Purpose

Expresses Flag-tagged D. melanogaster Hel25E

Depositor

Jeremy Wilusz

Article

Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

Insert

Hel25E (Hel25E Fly)

Tags

Flag

Expression

Insect

Promoter

Metallothionein Promoter (pMT)

Available Since

Oct. 4, 2020

Availability

Academic Institutions and Nonprofits only

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Hy_pMT Flag-Hel25E K91N

Plasmid

#110121

Purpose

Expresses Flag-tagged D. melanogaster Hel25E with K91N mutation that greatly reduces helicase activity

Depositor

Jeremy Wilusz

Article

Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

Insert

Hel25E (Hel25E Fly)

Tags

Flag

Expression

Insect

Mutation

K91N mutation

Promoter

Metallothionein Promoter (pMT)

Available Since

Sept. 27, 2020

Availability

Academic Institutions and Nonprofits only

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Hy_pMT Flag-Hel25E D193E

Plasmid

#110120

Purpose

Expresses Flag-tagged D. melanogaster Hel25E with D193E mutation that greatly reduces ATP binding affinity

Depositor

Jeremy Wilusz

Article

Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

Insert

Hel25E (Hel25E Fly)

Tags

Flag

Expression

Insect

Mutation

D193E mutation

Promoter

Metallothionein Promoter (pMT)

Available Since

Sept. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pTIGER_tdTomato::3xFLAG::dCrk

Plasmid

#131138

Purpose

UASp construct for over-expression of Drosophila Crk with N-terminal tandem Tomato and 3X FLAG tag. Compatible with PhiC31-mediated site-specific integration or classical P-element insertion.

Depositor

Mark Peifer

Article

Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

Insert

Crk (Crk Fly)

Tags

3XFLAG and tdTomato

Expression

Insect

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pAc-sgRNA-Cas9.scr_2

Plasmid

#190704

Purpose

Expresses scrambled sgRNA and Cas9-Puro in Drosophila S2 cells

Depositor

David Bartel

Article

Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

Insert

Scrambled sgRNA 2

Use

CRISPR

Expression

Insect

Promoter

Drosophila U6

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pGEM-T Dmel Or82a

Plasmid

#59678

Purpose

Probe for Drosophila melanogaster Or82a expression

Depositor

Richard Axel, Leslie Vosshall

Article

Vosshall et al Cell. 1999 Mar 5;96(5):725-36.

Insert

Or82a (Or82a Fly)

Expression

Bacterial

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only
pBSc + Shab

Plasmid

#16143

Depositor

Larry Salkoff

Article

Butler et al Science. 1989 Feb 17. 243(4893):943-7.

Insert

Shab-PA (Shab Fly)

Use

Xenopus oocyte expression

Mutation

N/A

Available Since

Jan. 10, 2008

Availability

Academic Institutions and Nonprofits only
dishevelled

Plasmid

#38342

Depositor

Norbert Perrimon

Article

Klingensmith et al Genes Dev. 1994 Jan;8(1):118-30.

Insert

dishevelled (dsh Fly)

Expression

Bacterial

Available Since

Aug. 29, 2012

Availability

Academic Institutions and Nonprofits only
CMV-dH3-YFP

Plasmid

#8694

Depositor

Kami Ahmad

Article

Janicki et al Cell 2004 Mar 5;116(5):683-98.

Insert

H3 (His3:CG31613 Fly)

Tags

YFP

Expression

Mammalian

Available Since

June 30, 2005

Availability

Academic Institutions and Nonprofits only

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pUAST tdTomato Rab11 WT

Plasmid

#53473

Depositor

Matthew Scott

Insert

Rab11 (Rab11 Fly)

Tags

tdtomato

Expression

Insect

Promoter

GAL4

Available Since

June 4, 2014

Availability

Academic Institutions and Nonprofits only