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Plasmid#203817PurposeDNA donor for transposition, shuttle vector for Corynebacterium glutamicum / E. coliDepositorInsertMini-Tn, Spec cassette
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCgVchcrRNA
Plasmid#203815PurposeExpression of CRISPR RNA, shuttle vector for Corynebacterium glutamicum / E. coliDepositorInsertCRISPR(BsaI)
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRD424
Plasmid#178202PurposeExpression of Rok from the hns promoterDepositorInsertphns + rok
UseTagsExpressionBacterialMutationPromoterhns promoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRD410
Plasmid#178200PurposeExpression of sRok from the hns promoterDepositorInsertphns + srok
UseTagsExpressionBacterialMutationPromoterhns promoterAvailable sinceNov. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28b-MBP-bhaB5
Plasmid#167812PurposeExpresses His6- and MBP-tagged BhaB5 in E. coliDepositorInsertBhaB5
UseTagsHis6, MBPExpressionBacterialMutationPromoterT7Available sinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSunS 1-335
Plasmid#171673PurposeSunS 1-335 expression in E. coliDepositorInsertGlycosyltransferase SunS (sunS Bacillus subtilis (strain 168))
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceAug. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJT174_GalL_A3A-Y130F-BE3
Plasmid#145123PurposeExpressing base editor A3A-Y130F-BE3 in yeast cellsDepositorInsertA3A(Y130F)-BE3
UseCRISPRTagsExpressionYeastMutationA3A(Y130F); spCas9(D10A)PromoterGalLAvailable sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJT173_GalL_A3A-R128A-BE3
Plasmid#145122PurposeExpressing base editor A3A-R128A-BE3 in yeast cellsDepositorInsertA3A(R128A)-BE3
UseCRISPRTagsExpressionYeastMutationA3A(R128A); spCas9(D10A)PromoterGalLAvailable sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJT113_GalL_cCDA1-xBE3
Plasmid#145079PurposeExpressing base editor cCDA1-xBE3 in yeast cellsDepositorInsertcCDA1-xBE3
UseCRISPRTagsExpressionYeastMutationspCas9(D10A, A262T, R324L, S409I, E480K, E543D, M…PromoterGalLAvailable sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ291-D573A
Plasmid#129676PurposeGateway entry clone for CRISPR-Cas12b systemsDepositorInsertBthCas12b
UseCRISPR; Gateway compatible bthcas12b entry cloneTagsExpressionPlantMutationD573A; BthCas12b is rice codon optimizedPromoterAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ291-D951A
Plasmid#129677PurposeGateway entry clone for CRISPR-Cas12b systemsDepositorInsertBthCas12b
UseCRISPR; Gateway compatible bthcas12b entry cloneTagsExpressionPlantMutationD951A; BthCas12b is rice codon optimizedPromoterAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ291-E827A
Plasmid#129678PurposeGateway entry clone for CRISPR-Cas12b systemsDepositorInsertBthCas12b
UseCRISPR; Gateway compatible bthcas12b entry cloneTagsExpressionPlantMutationE827A; BthCas12b is rice codon optimizedPromoterAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVR2-m4
Plasmid#84723PurposepVR2 but with G to U and A to C mutations following the riboswitch aptamerDepositorInsertsE. coli RecA promoter
stretch lacking A's followed by 5' UTR of queC gene
UseTagsExpressionBacterialMutationG to U mutation at the first nucleotide after the…PromoterAvailable sinceMarch 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVR2-m5
Plasmid#84724PurposepVR2 but with an insertion of 1 U immediately following the riboswitch aptamerDepositorInsertsE. coli RecA promoter
stretch lacking A's followed by 5' UTR of queC gene
UseTagsExpressionBacterialMutationinsertion of 1 U between positions 39 and 40 of a…PromoterAvailable sinceMarch 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVR2-m6
Plasmid#84725PurposepVR2 but with an insertion of 2 U's immediately following the riboswitch aptamerDepositorInsertsE. coli RecA promoter
stretch lacking A's followed by 5' UTR of queC gene
UseTagsExpressionBacterialMutationinsertion of 2 Us between positions 39 and 40 of …PromoterAvailable sinceMarch 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVR2-m7
Plasmid#84726PurposepVR2 but with a G to U mutation immediately following the riboswitch aptamerDepositorInsertsE. coli RecA promoter
stretch lacking A's followed by 5' UTR of queC gene
UseTagsExpressionBacterialMutationG to U mutation at position 41 of annotated "…PromoterAvailable sinceMarch 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVR2-m8
Plasmid#84727PurposepVR2 but with two G to U mutations immediately following the riboswitch aptamerDepositorInsertsE. coli RecA promoter
stretch lacking A's followed by 5' UTR of queC gene
UseTagsExpressionBacterialMutationG to U mutations at positions 40 and 41 of annota…PromoterAvailable sinceMarch 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVR2
Plasmid#84719PurposeE. coli RecA promoter followed by stretch with no A's followed by riboswitch for single-round transcriptionDepositorInsertsE. coli RecA promoter
stretch lacking A's followed by 5' UTR of queC gene
UseTagsExpressionBacterialMutation12 nt stretch lacking A's inserted before 5&…PromoterAvailable sinceMarch 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
p1G108CFa
Plasmid#85089PurposeExpresses PCNA1 variant (G108C) fused to an FAD domain of P450 BM3 (A74G/C773S/C810S) in E. coliDepositorInsertThe G108C variant of PCNA1 fused to an FAD domain of P450 BM3
UseTagsHis6 tagExpressionBacterialMutationG108C in PCNA1, A74G/C773S/C810S in FAD domain of…PromoterT7 promoterAvailable sinceNov. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pVRa31_34
Plasmid#49687DepositorInsertECF31_34
UseSynthetic BiologyTagsHis6-PreScissionExpressionBacterialMutationCodon optimized for E.coliPromoterpT7-lacOAvailable sinceJan. 6, 2014AvailabilityAcademic Institutions and Nonprofits only