We narrowed to 664 results for: Bacillus

1. pAB085d7

   Plasmid

   #79211

   Purpose

   Directed Evolution of Protein-Protein Interactions

   Depositor

   David Liu

   Article

   Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

   Insert

   PlacZ-opt (OR1) luxAB; Ppro1 434cI-TnCAD-F3
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PlacZ-opt (OR1); Ppro1

   Available since

   Dec. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAB085d6

   Plasmid

   #79210

   Purpose

   Directed Evolution of Protein-Protein Interactions

   Depositor

   David Liu

   Article

   Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

   Insert

   PlacZ-opt (OR1) luxAB; Ppro1 434cI-TnTBR3-F3
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PlacZ-opt (OR1); Ppro1

   Available since

   Dec. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPK1E-2+t-gfp

   Plasmid

   #48132

   Purpose

   Shuttle vector E. coli/B.meg.; gfp-expression under control of RNAP-K1E-inducible promoter

   Depositor

   Dieter Jahn

   Article

   Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

   Insert

   gfp
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 21, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFNC-5

   Plasmid

   #233297

   Purpose

   Plasmid encoding the BxbI phage integrase. It can be used to remove the antibiotic resistance cassette integrated with pCIFR. TcR, easily curable via sucrose counterselection.

   Depositor

   Pablo Ivan Nikel

   Article

   Federici et al Metab Eng. 2025 Jan 6;88:180-195. doi: 1

   Inserts

   BxbI phage integrase
   sacB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PEM7 and unknown

   Available since

   April 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFNC-6

   Plasmid

   #227669

   Purpose

   Plasmid encoding the BxbI phage integrase. It can be used to remove the antibiotic resistance cassette integrated with pCIFR. GmR, easily curable via sucrose counterselection.

   Depositor

   Pablo Ivan Nikel

   Article

   Federici et al Metab Eng. 2025 Jan 6;88:180-195. doi: 1

   Inserts

   BxbI phage integrase
   sacB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PEM7

   Available since

   Jan. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCgVchDonor

   Plasmid

   #203817

   Purpose

   DNA donor for transposition, shuttle vector for Corynebacterium glutamicum / E. coli

   Depositor

   Sheng Yang

   Article

   Yang et al ACS Synth Biol. 2023 Jul 21;12(7):2198-2

   Insert

   Mini-Tn, Spec cassette
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRD424

   Plasmid

   #178202

   Purpose

   Expression of Rok from the hns promoter

   Depositor

   Remus Dame

   Article

   Erkelens et al Nucleic Acids Res. 2022 Nov 21. pii: 683

   Insert

   phns + rok
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   hns promoter

   Available since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRD410

   Plasmid

   #178200

   Purpose

   Expression of sRok from the hns promoter

   Depositor

   Remus Dame

   Article

   Erkelens et al Nucleic Acids Res. 2022 Nov 21. pii: 683

   Insert

   phns + srok
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   hns promoter

   Available since

   Nov. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28b-MBP-bhaB5

   Plasmid

   #167812

   Purpose

   Expresses His6- and MBP-tagged BhaB5 in E. coli

   Depositor

   Wilfred van der Donk

   Article

   Daniels et al Nat Chem. 2022 Jan;14(1):71-77. doi: 10.

   Insert

   BhaB5
   

   Use

   Tags

   His6, MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSunS 1-335

    Plasmid

    #171673

    Purpose

    SunS 1-335 expression in E. coli

    Depositor

    Wilfred van der Donk

    Article

    Fujinami et al Cell Chem Biol. 2021 Dec 16;28(12):1740-

    Insert

    Glycosyltransferase SunS (sunS Bacillus subtilis (strain 168))
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJT174_GalL_A3A-Y130F-BE3

    Plasmid

    #145123

    Purpose

    Expressing base editor A3A-Y130F-BE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

    Insert

    A3A(Y130F)-BE3
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    A3A(Y130F); spCas9(D10A)

    Promoter

    GalL

    Available since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJT173_GalL_A3A-R128A-BE3

    Plasmid

    #145122

    Purpose

    Expressing base editor A3A-R128A-BE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

    Insert

    A3A(R128A)-BE3
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    A3A(R128A); spCas9(D10A)

    Promoter

    GalL

    Available since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJT113_GalL_cCDA1-xBE3

    Plasmid

    #145079

    Purpose

    Expressing base editor cCDA1-xBE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

    Insert

    cCDA1-xBE3
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    spCas9(D10A, A262T, R324L, S409I, E480K, E543D, M…

    Promoter

    GalL

    Available since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pYPQ291-D573A

    Plasmid

    #129676

    Purpose

    Gateway entry clone for CRISPR-Cas12b systems

    Depositor

    Yiping Qi

    Article

    Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

    Insert

    BthCas12b
    

    Use

    CRISPR; Gateway compatible bthcas12b entry clone

    Tags

    Expression

    Plant

    Mutation

    D573A; BthCas12b is rice codon optimized

    Promoter

    Available since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pYPQ291-D951A

    Plasmid

    #129677

    Purpose

    Gateway entry clone for CRISPR-Cas12b systems

    Depositor

    Yiping Qi

    Article

    Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

    Insert

    BthCas12b
    

    Use

    CRISPR; Gateway compatible bthcas12b entry clone

    Tags

    Expression

    Plant

    Mutation

    D951A; BthCas12b is rice codon optimized

    Promoter

    Available since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pYPQ291-E827A

    Plasmid

    #129678

    Purpose

    Gateway entry clone for CRISPR-Cas12b systems

    Depositor

    Yiping Qi

    Article

    Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

    Insert

    BthCas12b
    

    Use

    CRISPR; Gateway compatible bthcas12b entry clone

    Tags

    Expression

    Plant

    Mutation

    E827A; BthCas12b is rice codon optimized

    Promoter

    Available since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pVR2-m4

    Plasmid

    #84723

    Purpose

    pVR2 but with G to U and A to C mutations following the riboswitch aptamer

    Depositor

    Nils Walter

    Article

    Widom et al Mol Cell. 2018 Nov 1;72(3):541-552.e6. d

    Inserts

    E. coli RecA promoter
    stretch lacking A's followed by 5' UTR of queC gene
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    G to U mutation at the first nucleotide after the…

    Promoter

    Available since

    March 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pVR2-m5

    Plasmid

    #84724

    Purpose

    pVR2 but with an insertion of 1 U immediately following the riboswitch aptamer

    Depositor

    Nils Walter

    Article

    Widom et al Mol Cell. 2018 Nov 1;72(3):541-552.e6. d

    Inserts

    E. coli RecA promoter
    stretch lacking A's followed by 5' UTR of queC gene
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    insertion of 1 U between positions 39 and 40 of a…

    Promoter

    Available since

    March 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pVR2-m6

    Plasmid

    #84725

    Purpose

    pVR2 but with an insertion of 2 U's immediately following the riboswitch aptamer

    Depositor

    Nils Walter

    Article

    Widom et al Mol Cell. 2018 Nov 1;72(3):541-552.e6. d

    Inserts

    E. coli RecA promoter
    stretch lacking A's followed by 5' UTR of queC gene
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    insertion of 2 Us between positions 39 and 40 of …

    Promoter

    Available since

    March 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pVR2-m7

    Plasmid

    #84726

    Purpose

    pVR2 but with a G to U mutation immediately following the riboswitch aptamer

    Depositor

    Nils Walter

    Article

    Widom et al Mol Cell. 2018 Nov 1;72(3):541-552.e6. d

    Inserts

    E. coli RecA promoter
    stretch lacking A's followed by 5' UTR of queC gene
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    G to U mutation at position 41 of annotated "…

    Promoter

    Available since

    March 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge