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pCIneo-lambdaN-HA-CeSmg5_S

Plasmid

#147456

Purpose

Mammalian Expression of CeSmg5

Depositor

Elisa Izaurralde

Article

Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

Insert

CeSmg5 (smg-5 Nematode)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-CeSmg5-G91E_S

Plasmid

#147457

Purpose

Mammalian Expression of CeSmg5-G91E

Depositor

Elisa Izaurralde

Article

Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

Insert

CeSmg5-G91E (smg-5 Nematode)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-CeSmg5-Q94E_S

Plasmid

#147459

Purpose

Mammalian Expression of CeSmg5-Q94E

Depositor

Elisa Izaurralde

Article

Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

Insert

CeSmg5-Q94E (smg-5 Nematode)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-V5-SBP-CeSmg7_S

Plasmid

#147465

Purpose

Mammalian Expression of CeSmg7

Depositor

Elisa Izaurralde

Article

Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

Insert

CeSmg7 (smg-7 Nematode)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-V5-SBP-CeSmg7-G80E_S

Plasmid

#147466

Purpose

Mammalian Expression of CeSmg7-G80E

Depositor

Elisa Izaurralde

Article

Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

Insert

CeSmg7-G80E (smg-7 Nematode)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-V5-SBP-CeSmg7-T83E_S

Plasmid

#147467

Purpose

Mammalian Expression of CeSmg7-T83E

Depositor

Elisa Izaurralde

Article

Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

Insert

CeSmg7-T83E (smg-7 Nematode)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1-CeSMG5_1-420_S

Plasmid

#147468

Purpose

Bacterial Expression of CeSMG5_1-420

Depositor

Elisa Izaurralde

Article

Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

Insert

CeSMG5_1-420 (smg-5 Nematode)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuetMP-CeSmg7_1-395_S

Plasmid

#147475

Purpose

Bacterial Expression of CeSmg7_1-395

Depositor

Elisa Izaurralde

Article

Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

Insert

CeSmg7_1-395 (smg-7 Nematode)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pM159

Plasmid

#173215

Purpose

Expresses dCas9-sfGFP10

Depositor

Hajin Kim

Article

Chaudhary et al Genome Res. 2020 Sep;30(9):1306-1316. do

Insert

dCas9-sfGFP10M2

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-G-Cdc42

Plasmid

#180582

Purpose

ddGFP sensor of Cdc42

Depositor

Won Do Heo

Article

Kim J et al Nat Commun. 2019 Jan 14;10(1):211. doi:

Insert

ddGFP sensor of Cdc42

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-CeSmg5-H116E_S

Plasmid

#147458

Purpose

Mammalian Expression of CeSmg5-H116E

Depositor

Elisa Izaurralde

Article

Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

Insert

CeSmg5-H116E (smg-5 Nematode)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pDB4924

Plasmid

#174012

Purpose

pDUAL plasmid expressing Pil1-mCherry from 41nmt1 promoter

Depositor

Li-Lin Du

Article

Yu et al J Cell Sci. 2021 Oct 1;134(19):jcs258774

Insert

Pil1-mCherry

Use

Tags

Pil1, mCherry

Expression

Yeast

Mutation

Promoter

41nmt1 and 41nmt1p

Available since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pM80

Plasmid

#173173

Purpose

HA-dCas9

Depositor

Hajin Kim

Article

Chaudhary et al Genome Res. 2020 Sep;30(9):1306-1316. do

Insert

dCas9

Use

CRISPR and Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 31, 2021

Availability

Academic Institutions and Nonprofits only

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Scarlet I-FRET 10-Cerulean3

Plasmid

#139374

Purpose

FRET Standard

Depositor

Richard Day

Article

Day et al PLoS One. 2021 Dec 23;16(12):e0261660. d

Insert

Cerulean3 N1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 28, 2020

Availability

Academic Institutions and Nonprofits only

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pF3BGX-mTurquoise2

Plasmid

#138390

Purpose

RMCE vector for generating C-terminal mTurqouise2 fusion

Depositor

Shu Kondo

Article

Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

Insert

mTurquoise2

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 19, 2020

Availability

Academic Institutions and Nonprofits only

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FLAG-MBP1-K295TAG-W340A.pcDNA3-k

Plasmid

#126627

Purpose

Expresses MBP in mammalian cells. Amber codon at AA# 295 as a noncanonical amino acid incorporation site.

Depositor

Sharona Gordon

Article

Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1

Insert

Maltose Binding Protein

Use

Tags

FLAG

Expression

Mammalian

Mutation

Amino acid mutations: K295 to Amber codon and W34…

Promoter

CMV

Available since

July 23, 2019

Availability

Academic Institutions and Nonprofits only

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pOGG030

Plasmid

#113993

Purpose

Level 0 module. IPTG-inducible T7RNAP promoter for BL21 protein purification

Depositor

Philip Poole

Article

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Insert

IPTG-inducible T7RNAP promoter

Use

Tags

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Promoter

Available since

Jan. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pOGG082

Plasmid

#114000

Purpose

Level 0 module. NifH promoter for nodule-specific expression

Depositor

Philip Poole

Article

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Insert

NifH promoter

Use

Tags

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Promoter

Available since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pTorPE-Y-GECO2m

Plasmid

#105066

Purpose

A yellow fluorescent Ca2+ indicator variant with medium binding affinity and kinetics for expression in bacterial cells

Depositor

Robert Campbell

Article

Zhao et al Sci Rep. 2018 Aug 6;8(1):11758. doi: 10.

Insert

Y-GECO2m

Use

Tags

6x His tag

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

March 22, 2018

Availability

Academic Institutions and Nonprofits only

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pTorPE-Y-GECO2s

Plasmid

#105067

Purpose

A yellow fluorescent Ca2+ indicator variant with high binding affinity and slow kinetics for expression in bacterial cells

Depositor

Robert Campbell

Article

Zhao et al Sci Rep. 2018 Aug 6;8(1):11758. doi: 10.

Insert

Y-GECO2s

Use

Tags

6x His tag

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

Availability

Academic Institutions and Nonprofits only

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