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His6-TEV-LplA W37V and T57I

Plasmid

#248818

Purpose

Plasmid used for bacterial expression of LplA variants. TEV cleavage site enables removal of N-terminal His6 tag.

Depositor

Alice Ting

Article

Cavanagh et al Science. 2026 Jan 8:eadv7953. doi: 10.11

Insert

LplA

Tags

6xHis-TEV

Expression

Bacterial

Mutation

W37V and T57I

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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His6-TEV-LplA W37V and F147S

Plasmid

#248820

Purpose

Plasmid used for bacterial expression of LplA variants. TEV cleavage site enables removal of N-terminal His6 tag.

Depositor

Alice Ting

Article

Cavanagh et al Science. 2026 Jan 8:eadv7953. doi: 10.11

Insert

LplA

Tags

6xHis-TEV

Expression

Bacterial

Mutation

W37V and F147S

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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His6-TEV-LplA W37V and E265R

Plasmid

#248824

Purpose

Plasmid used for bacterial expression of LplA variants. TEV cleavage site enables removal of N-terminal His6 tag.

Depositor

Alice Ting

Article

Cavanagh et al Science. 2026 Jan 8:eadv7953. doi: 10.11

Insert

LplA

Tags

6xHis-TEV

Expression

Bacterial

Mutation

W37V and E265R

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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His6-TEV-LplA W37V and Y139S

Plasmid

#248822

Purpose

Plasmid used for bacterial expression of LplA variants. TEV cleavage site enables removal of N-terminal His6 tag.

Depositor

Alice Ting

Article

Cavanagh et al Science. 2026 Jan 8:eadv7953. doi: 10.11

Insert

LplA

Tags

6xHis-TEV

Expression

Bacterial

Mutation

W37V and Y139S

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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His-HA-E2p-YFP

Plasmid

#248827

Purpose

Plasmid used to express E2p, a site-specific acceptor protein for LplA, in HEK293T cells. Used to evaluate specific labeling activity of LplA variants.

Depositor

Alice Ting

Article

Cavanagh et al Science. 2026 Jan 8:eadv7953. doi: 10.11

Insert

E2P

Tags

6xHis-HA and YFP

Expression

Mammalian

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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His-LAP-BFP-CAAX

Plasmid

#248825

Purpose

Plasmid used to express LplA's site-specific substrate LAP2 (13-amino acid acceptor peptide) in mammalian cells. CAAX targets it to the inner leaflet of the plasma membrane.

Depositor

Alice Ting

Article

Cavanagh et al Science. 2026 Jan 8:eadv7953. doi: 10.11

Insert

LplA

Tags

6xHis and BFP-CAAX

Expression

Mammalian

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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His6-mCherry-Flag-LplAW37V and F147S

Plasmid

#248808

Purpose

Constructs used for LplA-catalyzed labeling in mammalian cells. LplA is expressed as a fusion with mCherry.

Depositor

Alice Ting

Article

Cavanagh et al Science. 2026 Jan 8:eadv7953. doi: 10.11

Insert

LplA

Tags

6xHis-mCherry-Flag

Expression

Mammalian

Mutation

W37V and F147S

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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His6-TEV-LplA W37V and A48N

Plasmid

#248823

Purpose

Plasmid used for bacterial expression of LplA variants. TEV cleavage site enables removal of N-terminal His6 tag.

Depositor

Alice Ting

Article

Cavanagh et al Science. 2026 Jan 8:eadv7953. doi: 10.11

Insert

LplA

Tags

6xHis-TEV

Expression

Bacterial

Mutation

W37V and A48N

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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His6-TEV-LplA W37V and L172A

Plasmid

#248815

Purpose

Plasmid used for bacterial expression of LplA variants. TEV cleavage site enables removal of N-terminal His6 tag.

Depositor

Alice Ting

Article

Cavanagh et al Science. 2026 Jan 8:eadv7953. doi: 10.11

Insert

LplA

Tags

6xHis-TEV

Expression

Bacterial

Mutation

W37V and L172A

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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His6-TEV-LplA W37V

Plasmid

#248814

Purpose

Plasmid used for bacterial expression of LplA variants. TEV cleavage site enables removal of N-terminal His6 tag.

Depositor

Alice Ting

Article

Cavanagh et al Science. 2026 Jan 8:eadv7953. doi: 10.11

Insert

LplA

Tags

6xHis-TEV

Expression

Bacterial

Mutation

W37V

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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His6-TEV-LplA

Plasmid

#248813

Purpose

Plasmid used for bacterial expression of LplA variants. TEV cleavage site enables removal of N-terminal His6 tag.

Depositor

Alice Ting

Article

Cavanagh et al Science. 2026 Jan 8:eadv7953. doi: 10.11

Insert

LplA

Tags

6xHis-TEV

Expression

Bacterial

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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His6-mCherry-Flag-LplA Wild Type

Plasmid

#248801

Purpose

Constructs used for LplA-catalyzed labeling in mammalian cells. LplA is expressed as a fusion with mCherry.

Depositor

Alice Ting

Article

Cavanagh et al Science. 2026 Jan 8:eadv7953. doi: 10.11

Insert

LplA

Tags

6xHis-mCherry-Flag

Expression

Mammalian

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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His6-mCherry-Flag-LplAW37V and L172A

Plasmid

#248803

Purpose

Constructs used for LplA-catalyzed labeling in mammalian cells. LplA is expressed as a fusion with mCherry.

Depositor

Alice Ting

Article

Cavanagh et al Science. 2026 Jan 8:eadv7953. doi: 10.11

Insert

LplA

Tags

6xHis-mCherry-Flag

Expression

Mammalian

Mutation

W37V and L172A

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pHR iRFP-ZtSH2

Plasmid

#188627

Purpose

Fluorescent reporter for CD3ε/ZtSH2 pYtag biosensor

Depositor

Jared Toettcher

Article

Farahani et al Elife. 2023 May 22;12:e82863. doi: 10.75

Insert

ZtSH2

Use

Lentiviral

Tags

iRFP

Expression

Mammalian

Promoter

SFFV

Available Since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pCA24N_6xHis-TEV-rpsA

Plasmid

#83585

Purpose

Express E. coli ribosomal protein S1 with a TEV-cleavable, N-terminal His tag

Depositor

Nils Walter

Article

Lund et al Nucleic Acids Res. 2019 Dec 13. pii: 567

Insert

rpsA (rpsA Escherichia coli)

Tags

His tag

Expression

Bacterial

Promoter

p-T5 Lac

Available Since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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TR-Csy4

Plasmid

#80601

Purpose

Expresses Csy4 from the CBA promoter. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.

Depositor

Aravind Asokan

Article

Borchardt et al RNA. 2015 Nov;21(11):1921-30. doi: 10.12

Insert

Csy4

Use

AAV

Expression

Mammalian

Promoter

CBA

Available Since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-CamKII-DIO-mCherry

Plasmid

#190241

Purpose

Expresses mCherry flanked by DIO

Depositor

Young J Yoon

Article

Nasrallah et al Proc Natl Acad Sci U S A. 2022 Aug 9;119

Insert

mCherry

Use

AAV

Tags

N/A

Promoter

mouse CamKII alpha

Available Since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pHR Clover-VISH2

Plasmid

#188629

Purpose

Fluorescent reporter for SLP76/VISH2 pYtag biosensor

Depositor

Jared Toettcher

Article

Farahani et al Elife. 2023 May 22;12:e82863. doi: 10.75

Insert

VISH2

Use

Lentiviral

Tags

Clover

Expression

Mammalian

Promoter

SFFV

Available Since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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p3PA-Puro-iTk

Plasmid

#109067

Purpose

inserts three polyA sites to end transcription of a targetted gene and a puromycin cassette for selection

Depositor

Eric Lander

Article

Engreitz et al Nature. 2016 Nov 17;539(7629):452-455. d

Type

Empty backbone

Expression

Mammalian

Promoter

EFS

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pPK700

Plasmid

#38146

Depositor

Patricia Kuwabara

Article

Soloviev et al Dev Biol. 2011 Mar 15;351(2):242-53. Epu

Insert

let-60p::ptc-3::gfp (ptc-3 Nematode)

Tags

EGFP

Expression

Worm

Promoter

let-60

Available Since

Oct. 25, 2012

Availability

Academic Institutions and Nonprofits only

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