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Plasmid#83067PurposeExpresses 6xHis tagged PP3359 under pBAD regulationDepositorInsertPP3359
UseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterpBADAvailable sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
SPDK3214
Plasmid#97406Purposebinary construct of ER-targeted GUS with sfCherry11 tag for plant expressionDepositorInsertER-GUS-sfCherry11
UseTagsER targetExpressionPlantMutationPromoterAvailable sinceSept. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCPP6534
Plasmid#128735PurposeGateway destination pUC18R6KT-Tn7T-PavrPto-GW-Cya-Km vector for integration into DC3000D36E genome glmS-linked attTn7 site any effector gene, expressed from avrPto promoter and with C-terminal HA tagDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPPC009.106
Plasmid#171142PurposeExpression of J106 scRNA on pBBR1-KmR plasmidDepositorInsertJ106 scRNA
UseCRISPRTagsExpressionBacterialMutationPromoterBBa_J23119Available sinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGK415-ÂphlTA2-Â1E
Plasmid#165970PurposeExpresses phlTA(2-Â1E) mutantDepositorInsertphlF
UseSynthetic BiologyTagsNuclear localization signal and three copies of V…ExpressionYeastMutationP5S, S6P, K86T, F109L, Q117R, E143K, codon-optimi…PromoterpPGK1 mutantAvailable sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD3C
Plasmid#86971PurposeExpresses PCNA3 protein fused phosphite dehydrogenase and P450cam in E. coliDepositorInsertPCNA3 fused to phosphite dehydrogenase and P450cam
UseTagsHis6 tagExpressionBacterialMutationE175A/A176R in PTDH, N106R in PCNA3, silent mutat…PromoterT7 promoterAvailable sinceMarch 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNVLTv2-pvdIJD-Prha-base
Plasmid#169244PurposeSuicide vector for integrating expression cassette in P. putida (L-rhamnose induction)DepositorInsertrhaRS
UseTagsExpressionBacterialMutationPromoterrhaSAvailable sinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCPP6533
Plasmid#128734PurposeGateway destination pUC18R6KT-Tn7T-PavrPto-GW-Cya-Km vector for integration into DC3000D36E genome glmS-linked attTn7 site any effector gene, expressed from avrPto promoter and with C-terminal Cya tagDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNS3_Ptrc::lasR_Plas::mNG
Plasmid#189573PurposeFluorescent reporter for Las activation by 3OC12-HSLDepositorInsertsTranscriptional activator lasR
Monomeric Neon Green
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterlas and trcAvailable sinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRS-BG42-GFP
Plasmid#220202Purposemini-Tn7 GFP expression vector - pBG42 promoterDepositorInsertmonomeric superfolder green fluorescent protein
UseCRISPRTagsExpressionBacterialMutationmonomeric superfolder green fluorescent proteinPromoterpBG42Available sinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRS-BG35-GFP
Plasmid#220201Purposemini-Tn7 GFP expression vector - pBG35 promoterDepositorInsertmonomeric superfolder green fluorescent protein
UseCRISPRTagsExpressionBacterialMutationmonomeric superfolder green fluorescent proteinPromoterpBG35Available sinceSept. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRS-AraC-GFP
Plasmid#220199Purposemini-Tn7 GFP expression vector - AraC/pBAD promoterDepositorInsertmonomeric superfolder green fluorescent protein
UseCRISPRTagsExpressionBacterialMutationmonomeric superfolder green fluorescent proteinPromoterAraC/pBADAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRS-XylS-GFP
Plasmid#220200Purposemini-Tn7 GFP expression vector - XylS/pM promoterDepositorInsertmonomeric superfolder green fluorescent protein
UseCRISPRTagsExpressionBacterialMutationmonomeric superfolder green fluorescent proteinPromoterXylS/pMAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSpyA-GFP
Plasmid#220189PurposesgRNA expression vector - pEM7 promoter with GFP in sgRNA siteDepositorInsertpEM7 promoter with GFP in sgRNA site
UseCRISPRTagsExpressionBacterialMutationmonomeric superfolder green fluorescent proteinPromoterpEM7Available sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSpyB-GFP
Plasmid#220190PurposesgRNA expression vector - pBG35 promoter with GFP in sgRNA siteDepositorInsertpBG35 promoter with GFP in sgRNA site
UseCRISPRTagsExpressionBacterialMutationmonomeric superfolder green fluorescent proteinPromoterpBG35Available sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSpyD-GFP
Plasmid#220192PurposesgRNA expression vector - pBG28 promoter with GFP in sgRNA siteDepositorInsertpBG28 promoter with GFP in sgRNA site
UseCRISPRTagsExpressionBacterialMutationmonomeric superfolder green fluorescent proteinPromoterpBG28Available sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLipoP1-NLS-3xmCitrine
Plasmid#192522PurposeFluorescent reporter for plant responses to bacterial infectionDepositorInsertPutative membrane lipoprotein (AT3G18250 Mustard Weed)
UseTags3xmCitrine and NLSExpressionPlantMutationPromoterLipoP1Available sinceJune 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPPC008.106
Plasmid#171141PurposeExpression of J106 scRNA on pBBR1-GmR plasmidDepositorInsertJ106 scRNA
UseCRISPRTagsExpressionBacterialMutationPromoterBBa_J23119Available sinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet1-tglCDEF
Plasmid#134677PurposeExpresses TglC, TglD, TglE, TglF in E. ColiDepositorInsertsTglC
TglD
TglE
TglF
UseTagsExpressionBacterialMutationPromoterT7Available sinceJuly 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet1-tglB
Plasmid#134676PurposeExpresses His6-TglBin E. ColiDepositorInsertTglB
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceJuly 17, 2020AvailabilityAcademic Institutions and Nonprofits only