We narrowed to 14,039 results for: TIM

1. pCS2-zfCSF3a

   Plasmid

   #168201

   Purpose

   Express zebrafish Csf3a, mRNA synthesis

   Depositor

   Petr Bartunek

   Article

   Stachura et al Blood. 2013 Dec 5;122(24):3918-28. doi:

   Insert

   Csf3a (csf3a Zebrafish)
   

   Use

   Vertebrate expression (zebrafish, chicken, xenopu…

   Expression

   Mammalian

   Mutation

   Deletion of A27

   Available Since

   Aug. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pVMG0217_Cq-U6-2b_2xBbsI-gRNA

   Plasmid

   #169339

   Purpose

   Expression of gRNA in Culex quinquefasciatus or other mosquitoes

   Depositor

   Valentino Gantz

   Article

   Feng et al Nat Commun. 2021 May 20;12(1):2960. doi:

   Insert

   pVMG0217_Cq-U6-2b_2xBbsI-gRNA
   

   Use

   CRISPR

   Expression

   Insect

   Promoter

   CPIJ039728

   Available Since

   May 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBI-CMV1-mir374b~421-CMV2-mir1

   Plasmid

   #164674

   Purpose

   Express miR-374b~421 and miR-1-1 in mammalian cells

   Depositor

   David Bartel

   Article

   Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

   Inserts

   miR-374b~421 (MIR374B Human)
   miR-1-1 (MIR1-1 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBI-CMV1-mir374b-CMV2-mir1

   Plasmid

   #164672

   Purpose

   Express miR-374b and miR-1-1 in mammalian cells

   Depositor

   David Bartel

   Article

   Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

   Inserts

   miR-374b (MIR374B Human)
   miR-1-1 (MIR1-1 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBI-CMV1-mir181b-1~125-CMV2-mir1

   Plasmid

   #164671

   Purpose

   Express miR-181b-1~125a and miR-1-1 in mammalian cells

   Depositor

   David Bartel

   Article

   Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

   Inserts

   miR-181b-1~125a (MIR181B1 Human)
   miR-1-1 (MIR1-1 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. KG#920

   Plasmid

   #110937

   Purpose

   Expresses INS-22-Emerald in the cholinergic motor neuron DA9 for imaging Dense Core Vesicles in a single neuron in a living animal

   Depositor

   Kenneth Miller

   Article

   Edwards et al Genetics. 2018 Nov;210(3):947-968. doi:

   Inserts

   mig-13 promoter
   INS-22-Emerald
   unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
   

   Expression

   Bacterial

   Available Since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTrichoGate-15

   Plasmid

   #160715

   Purpose

   For C-terminal tags with Trichoderma optimised Synthetic 3X-FLAG peptide bordered by BsaI restriction sites

   Depositor

   Artemio Mendoza

   Article

   Nogueira-Lopez et al Front Microbiol. 2019 Dec 10;10:2794. do

   Insert

   Trichoderma optimised Synthetic 3X-FLAG peptide
   

   Use

   Synthetic Biology

   Available Since

   March 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMIII-Neo-mChe

   Plasmid

   #153423

   Purpose

   Retroviral (MSCV) expression of mCherry fluorescent protein

   Depositor

   Maki Nakayama

   Article

   Mann et al Front Immunol. 2020 Apr 9;11:633. doi: 1

   Insert

   mCherry
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   Aug. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. FoxFTVC+bpFOG_MEK-S216D, S220E

   Plasmid

   #107522

   Purpose

   Constitutive activation of FGF/MAPK pathway by MEK-S216D, S220E mutant

   Depositor

   Lionel Christiaen

   Article

   Razy-Krajka et al Elife. 2018 Feb 6;7. pii: 29656. doi: 10

   Insert

   MEK
   

   Mutation

   changed Serine 216 to Aspartic Acid and Serine 22…

   Promoter

   TVC-specific FoxF enhancer (FoxFTVC+bpFOG)

   Available Since

   April 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. BII-C3H2B

    Plasmid

    #133387

    Purpose

    Piggybac vector for SRIRACCHA-mediated mutation enrichment (surrogate target for HDR)

    Depositor

    Blair Madison

    Article

    Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

    Type

    Empty backbone

    Expression

    Mammalian

    Promoter

    CMV enhancer and hEf1a (CpG free)

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET20b-YbAnbu

    Plasmid

    #129637

    Purpose

    Expression plasmid of Anbu from Y. bercovieri (UNIPROT A0A0T9RXH3 variant with V14A and G107H mutations)

    Depositor

    Matthias Bochtler

    Article

    Piasecka et al J Mol Biol. 2018 Mar 2;430(5):611-627. d

    Insert

    YbAnbu
    

    Tags

    His6-tag

    Expression

    Bacterial

    Mutation

    V14A and G107H

    Promoter

    T7

    Available Since

    Nov. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET20b-YbAnbuT1A

    Plasmid

    #129638

    Purpose

    Expression plasmid of Anbu from Y. bercovieri (inactive mutant; (UNIPROT A0A0T9RXH3 variant with T1A, V14A and G107H mutations)

    Depositor

    Matthias Bochtler

    Article

    Piasecka et al J Mol Biol. 2018 Mar 2;430(5):611-627. d

    Insert

    YbAnbuT1A
    

    Tags

    His6-tag

    Expression

    Bacterial

    Mutation

    T1A, V14A and G107H

    Promoter

    T7

    Available Since

    Nov. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTAMACHis6_PiuD

    Plasmid

    #128953

    Purpose

    expresses PiuD in E.coli

    Depositor

    James Naismith

    Article

    Luscher et al Antimicrob Agents Chemother. 2018 May 25

    Insert

    Putative outer membrane receptor for iron transport (PiuD)
    

    Tags

    6xHis and TamA

    Expression

    Bacterial

    Mutation

    Signal peptide sequence has been removed (1-105)

    Promoter

    T7

    Available Since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCarCBADE

    Plasmid

    #117757

    Purpose

    Codon optimized CarCBADE enzymes from Pectobacterium carotovorum in pBbaA5k backbone

    Depositor

    Greg Bokinsky

    Article

    Shomar et al Nat Chem Biol. 2018 Aug;14(8):794-800. d

    Insert

    Codon optimised CarCBA, Protein CarD, Ferredoxin CarE
    

    Expression

    Bacterial

    Promoter

    PLac(UV5)

    Available Since

    Nov. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHES 946

    Plasmid

    #112644

    Purpose

    blue light inducible cryptochrome based optogenetic expression system

    Depositor

    Hana El-Samad

    Article

    Harrigan et al Cell. 2018 Oct 18;175(3):877-886.e10. do

    Insert

    RTG3AD-GSx7-CIB1-SV40NLS (CIB1 Mustard Weed, Synthetic, Budding Yeast)
    

    Expression

    Yeast

    Promoter

    ADH1

    Available Since

    Aug. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1-PsuACR_353-EYFP

    Plasmid

    #111076

    Purpose

    Anion channelrhodopsin from Proteomonas sulcata (CCMP704) for expression in mammalian cells

    Depositor

    John Spudich

    Article

    Govorunova et al eNeuro. 2018 Jul 10;5(3). pii: eN-MNT-01

    Insert

    Anion channelrhodopsin PsuACR_353
    

    Tags

    EYFP

    Expression

    Mammalian

    Available Since

    June 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pONL-C1_RhoB

    Plasmid

    #65698

    Purpose

    Expresses ONL-RhoB in mammalian cells

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    RhoB (RHOB Human)
    

    Use

    Luciferase

    Tags

    ONL

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pYNL-C1_RhoB

    Plasmid

    #65696

    Purpose

    Expresses YNL-RhoB in mammalian cells

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    RhoB (RHOB Human)
    

    Use

    Luciferase

    Tags

    YNL

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pONL-N1_vinculin

    Plasmid

    #65692

    Purpose

    Expresses Vinculin-ONL in mammalian cells

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    vinculin (Vcl Mouse)
    

    Use

    Luciferase

    Tags

    ONL

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

20. pYNL-N1_vinculin

    Plasmid

    #65690

    Purpose

    Expresses Vinculin-YNL in mammalian cells

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    vinculin (Vcl Mouse)
    

    Use

    Luciferase

    Tags

    YNL

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 15, 2016

    Availability

    Academic Institutions and Nonprofits only