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pAAV-SCP1-dSa VPR mini.-2X snRP-1 Afp

Plasmid

#99696

Purpose

Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA for Afp, vector allows for strong activation of mouse Afp, can be packaged and delivered as AAV

Depositor

George Church

Insert

dCas9 and gRNA targeting Afp (Afp Synthetic)

Use

AAV

Tags

VPR mini

Mutation

dead Cas9

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-Ai14-luc

Plasmid

#87118

Purpose

AAV backbone plasmid including luc-pA knock-in donor and Ai14gRNA for HITI

Depositor

Juan Belmonte

Article

Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

Insert

U6-Ai14sgRNA-Luciferase-nEF-GFPKASH-hGHpA

Use

AAV, CRISPR, and Luciferase

Available Since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

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AiP14358 - pAAV-CAG-SYFP2-WPRE-SV40pA (Alias: CN4358)

Plasmid

#234712

Purpose

Direct-expressing SYFP2 AAV virus

Depositor

Tanya Daigle, Allen Institute for Brain Science, Jonathan Ting

Article

Kussick et al Cell Rep. 2025 Jun 24;44(6):115730. doi:

Insert

SYFP2

Use

AAV

Promoter

CAG

Available Since

June 27, 2025

Availability

Academic Institutions and Nonprofits only

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HCT163_pAAV-eHGT_3Xcore_1137m-minBGprom-ChR2(H134R)-EYFP-WPRE3-bGHpA

Plasmid

#233562

Purpose

Direct-expressing ChR2(H134R)-EYFP AAV virus

Depositor

Tanya Daigle, Allen Institute for Brain Science

Article

Kussick et al Cell Rep. 2025 Jun 24;44(6):115730. doi:

Insert

ChR2(H134R)-EYFP

Use

AAV

Promoter

minBglobin

Available Since

June 27, 2025

Availability

Academic Institutions and Nonprofits only

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AAV Syn-FLEX-coca-GlyR-IRES-mCherry

Plasmid

#242196

Purpose

Cre-dependent AAV vector for cocaine-gated chemogenetic anion channel

Depositor

Scott Sternson

Article

Gomez et al Nature. 2025 Aug 27. doi: 10.1038/s41586

Insert

Syn-FLEX-coca-GlyR-IRES-mCherry

Use

AAV

Expression

Mammalian

Mutation

L141G G175K Y217F

Promoter

Synapsin

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-FLEX-macaque OPN4-IRES-EGFP

Plasmid

#223253

Purpose

AAV construct for Cre-dependent (FLEX) expression of macaque melanopsin (Opn4, short isoform, GenBank:OP856641; hSyn promoter) and of EGFP via an IRES.

Depositor

Michael Do

Article

Liu et al Science. 2023 Jan 27;379(6630):376-381.

Insert

macaque OPN4-IRES-EGFP (OPN4 )

Use

AAV

Promoter

human Synapsin 1 (hSyn)

Available Since

Nov. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pGP-AAV-CAG-flex-iGABASnFR2(no bind)-WPRE

Plasmid

#218882

Purpose

AAV-mediated expression of improved GABA sensor control (no change in fluorescence)

Depositor

GENIE Project

Article

Kolb et al eLife. 2026 Mar 18;14:RP108319. doi: 10.

Insert

iGABASnFR2(no bind)

Use

AAV and Cre/Lox

Expression

Mammalian

Mutation

S99A F102G R168P

Promoter

CAG

Available Since

June 18, 2024

Availability

Academic Institutions and Nonprofits only

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pOTTC1618 pAAV SYN1 Nuc-EYFP miR-30 FF3

Plasmid

#135564

Purpose

An AAV vector expressing miR-30a shRNA vs FF luciferase and a nuclear EYFP reporter

Depositor

Christopher Richie

Article

Venniro et al Proc Natl Acad Sci U S A. 2020 Apr 7;117

Inserts

miR-30 FF3
Nuc-EYFP

Use

AAV

Tags

3xNLS

Expression

Mammalian

Promoter

SYN1 and hSYN1

Available Since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pOTTC1619 pAAV SYN1 Nuc-EYFP miR-30 SST

Plasmid

#135565

Purpose

An AAV vector expressing miR-30a shRNA vs rat SOM (aka SST) and a nuclear EYFP reporter

Depositor

Christopher Richie

Article

Venniro et al Proc Natl Acad Sci U S A. 2020 Apr 7;117

Inserts

miR-30 rat SST
Nuc-EYFP

Use

AAV

Tags

3xNLS

Expression

Mammalian

Promoter

SYN1 and hSYN1

Available Since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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AAV CMV-driven AcrIIA4-scaffold (2xBsmBI sites)

Plasmid

#120297

Purpose

AAV vector for expression of AcrIIA4 (no miR binding sites, control vector)

Depositor

Dominik Niopek

Article

Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

Insert

AcrIIA4

Use

AAV and CRISPR

Tags

FLAG

Expression

Mammalian

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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AAV CMV-driven AcrIIC3-scaffold (2xBsmBI sites)

Plasmid

#120301

Purpose

AAV vector for expression of AcrIIC3 (no miR binding sites, control vector)

Depositor

Dominik Niopek

Article

Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

Insert

AcrIIC3

Use

AAV and CRISPR

Expression

Mammalian

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-mCherry-SynaptoZip

Plasmid

#177318

Purpose

AAV expression of the synaptic activity-marker SynaptoZip fused to mCherry driven by human Synapsin promoter

Depositor

Antonio Malgaroli

Article

Lamanna et al Sci Rep. 2022 Jun 30;12(1):11055. doi: 1

Insert

mCherry-SynaptoZip (Vamp2 Rat)

Use

AAV

Tags

Myc and mCherry

Expression

Mammalian

Promoter

hSyn

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.FLEX.iGluSnFR3.v857.PDGFR

Plasmid

#196219

Purpose

Fluorescent reporter for glutamate, third generation, variant 857 in PDGFR backbone. iGluSnFR3.v857.PDGFR

Depositor

Kaspar Podgorski

Article

Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

Has Service

AAV2

Insert

pAAV CAG FLEX iGluSnFR3 v857.PDGFR

Use

AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

Tags

IgK-chain, Myc epi tag, and PDGFR TM Domain

Expression

Mammalian

Available Since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-UBC-S2-RAB_EKARev

Plasmid

#160726

Purpose

Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains RFP-based fluorescent reporter RAB-EKARev (ERK indicator), VSV-G tag, and S2 protein scaffold.

Depositor

Edward Boyden

Article

Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

Insert

S2-RAB_EKARev

Use

AAV

Expression

Mammalian

Mutation

N/A

Promoter

Human ubiquitin C (UBC) promoter

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-JEDI3sub-WPRE

Plasmid

#246619

Purpose

Genetically encoded voltage indicator (GEVI) JEDI3sub in an AAV production vector for neuron -specific expression using the promoter hSyn

Depositor

Francois St-Pierre

Article

Land et al Nat Methods. 2026 Apr 15. doi: 10.1038/s

Insert

JEDI3sub

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available Since

April 23, 2026

Availability

Academic Institutions and Nonprofits only

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AiP20027 - pAAV-AiE2182m_3xC2-minBG-iCre(R297T)-BGHpA

Plasmid

#220735

Purpose

AiE2182m_3xC2 is an optimized enhancer sequence, designed to induce cre-dependent recombination in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

iCre(R297T)

Use

AAV

Promoter

minBG

Available Since

Feb. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV_MLP-BC0250-luc2

Plasmid

#214666

Purpose

barcoded assay, internal control

Depositor

Michael Wehr

Article

Popovic et al STAR Protoc. 2024 Apr 16;5(2):102987. do

Insert

Adenovirus major late promoter (MLP) with barcode 0250

Use

AAV

Expression

Mammalian

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV.U6-sasgRNA(SapI)_Ple155-CI-EGFP-W3SL_BbsI(GGA)

Plasmid

#231367

Purpose

Ple155-driven EGFP, also encoding U6-driven sgRNA cassette, with SapI sites to clone crRNA sequence

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

EGFP

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.U6-sasgRNA(SapI)_Ple155-CI-mRuby2-W3SL_BbsI(GGA)

Plasmid

#231368

Purpose

Ple155-driven mRuby2, also encoding U6-driven sgRNA cassette, with SapI sites to clone crRNA sequence

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

mRuby2

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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AiP1045-pAAV-mscRE10-minBGpromoter-iCre-WPRE-hGHpA

Plasmid

#163477

Purpose

Direct-expressing iCre AAV Virus. Alias: AiP1045 - pAAV-AiE2010m-minBG-iCre-WPRE-HGHpA

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Article

Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

Insert

iCre

Use

AAV

Promoter

Beta Globin minimal promoter

Available Since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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