We narrowed to 9,514 results for: try

1. pCMV14-3X-Flag-SARS-CoV-2 S

   Plasmid

   #145780

   Purpose

   Over expression of SARS-CoV-2 S (spike) in mammalian cell line

   Depositor

   Zhaohui Qian

   Article

   Ou et al Nat Commun. 2020 Mar 27;11(1):1620. doi:

   Insert

   SARS-CoV-2 S (S SARS CoV 2)
   

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   Codon optimized and C-terminal 19 amino acids del…

   Promoter

   CMV

   Available Since

   May 6, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pHIV-IL13-IgG4hinge-PDGFR

   Plasmid

   #240245

   Purpose

   Lentiviral transfer plasmid encoding IL13 with IgG4 hinge and PDGFR TM domain

   Depositor

   Michael Birnbaum

   Article

   Dobson et al Nat Methods. 2022 Apr;19(4):449-460. doi

   Insert

   IL-13 (IL13 Human)
   

   Use

   Lentiviral

   Tags

   EGFP and HA

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   June 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCF140_Gag-SpyCas9-NLS

   Plasmid

   #225959

   Purpose

   CMV-Intron-Gag-SpyCas9-NLS. Expresses Gag-SpyCas9-NLS for VLP production.

   Depositor

   Christof Fellmann

   Article

   Ramadoss et al bioRxiv [Preprint]. 2024 Jun 26:2024.06.

   Insert

   CMV-Intron-Gag-SpyCas9-NLS
   

   Expression

   Mammalian

   Available Since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. 139H2_HC

   Plasmid

   #206201

   Purpose

   For recombinant expression of the full heavy chain of the Mouse anti-MUC1 antibody 139H2 in mammalian cells. Includes a C-terminal -His8 tag for purification.

   Depositor

   Joost Snijder

   Article

   Peng et al Life Sci Alliance. 2024 Mar 20;7(6):e202

   Insert

   anti-MUC1 antibody 139H2 heavy chain (Ighg1 Mouse)
   

   Tags

   -AAAHHHHHHHH

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. ACE2

   Plasmid

   #164219

   Purpose

   Expresses full length human ACE2 protein, along with TagBFP reporter and Puromycin selection marker

   Depositor

   Sriram Neelamegham

   Article

   Yang et al Elife. 2020 Oct 26;9. pii: 61552. doi: 1

   Insert

   ACE2 (ACE2 Human)
   

   Use

   Lentiviral

   Mutation

   silent mutation to remove internal EcoRI site (GA…

   Promoter

   CMV

   Available Since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pENTR1A-TAZ S89A

   Plasmid

   #179911

   Purpose

   Entry vector of TAZ S89A for gateway coloning.

   Depositor

   Xin Chen

   Article

   Wang et al J Hepatol. 2021 Aug 28. pii: S0168-8278(

   Insert

   WW Domain Containing Transcription Regulator 1 (WWTR1 Human)
   

   Use

   Entry vector

   Tags

   3X Flag-tag

   Mutation

   Serine 89 mutated to Alanine

   Available Since

   March 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEX-GST-BcPC-PLC

   Plasmid

   #224255

   Purpose

   Express N-terminal HRV 3C protease cleavable GST fused to Bacillus cereus PC-PLC in E.coli

   Depositor

   Chiaki Murakami

   Article

   Murakami et al Anal Biochem. 2017 Jun 1;526:43-49. doi:

   Insert

   Bacillus cereus phospholipase C (plC, plC Bacillus cereus ATCC 14579)
   

   Tags

   GST and HRV-3C (LEVLFQGP) site

   Expression

   Bacterial

   Mutation

   The gene of Bacillus cereus ATCC 14579 was used f…

   Promoter

   lac promoter

   Available Since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pENTR1A FKBP12F36V-OGT

   Plasmid

   #154287

   Purpose

   Entry vector encoding FKBP12(F36V)-2xHA-OGT (dTAG fused-O-GlcNAc Transferase)

   Depositor

   Suzanne Walker

   Article

   Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

   Insert

   O-GlcNAc Transferase (OGT Human)
   

   Use

   Gateway entry vector

   Tags

   2x HA and FKBP12(F36V)

   Available Since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCR8/GW_TOPO_Cdk12_Nterm_FlagHa

   Plasmid

   #127177

   Purpose

   N-terminal Flag- HA- tandem epitope tagged mouse Cdk12 transgene (NM_001109626.1 isoform) cloned into Gateway entry vector

   Depositor

   Phil Sharp

   Article

   Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi:

   Insert

   N-terminally Flag- HA- Epitope Tagged Mouse Cyclin Dependent Kinase 12 (Cdk12 Mouse)
   

   Use

   Entry vector with transgene for gateway cloning

   Tags

   Flag- HA- Tandem Epitopes

   Promoter

   None

   Available Since

   July 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pT7-G-AsCas12a crRNA-BsaI cassette (MSP3491)

    Plasmid

    #114067

    Purpose

    T7 promoter crRNA entry vector used for in vitro transcription of AsCas12a guides

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    entry vector for AsCas12a crRNAs
    

    Use

    In vitro transcription

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKK-TEV-CyOFP1

    Plasmid

    #105799

    Purpose

    Expression of your protein of interest in fusion with orange fluorescent protein at the C-terminus (cleavable by TEV) (PMID: 27240196). CyOFP1 is useful for multi-channel imaging.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    TEV-CyOFP1

    Expression

    Mammalian

    Available Since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. MAC-FURIN

    Plasmid

    #158386

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

    Insert

    furin, paired basic amino acid cleaving enzyme (FURIN Human)
    

    Tags

    MAC-tag

    Expression

    Mammalian

    Available Since

    Aug. 25, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pME-GcAMP5

    Plasmid

    #126569

    Purpose

    Multisite gateway middle entry clone containing zebrafish calcium reporter GcAMP5. Parton lab clone ANR.

    Depositor

    Rob Parton

    Article

    Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

    Insert

    GcAMP5
    

    Use

    Gateway entry clone

    Available Since

    Nov. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUC19-U6-BsmBI_cassette-Nme/Nme2Cas9-sgRNA (KAC32)

    Plasmid

    #133794

    Purpose

    U6 promoter sgRNA entry vector used for all NmeCas9 or Nme2Cas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to sgRNA architecture from Amrani et al. Genome Biology 2018

    Depositor

    Joseph Bondy-Denomy, Benjamin Kleinstiver

    Article

    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

    Insert

    NmeCas9 and Nme2Cas9 sgRNA entry vector
    

    Expression

    Mammalian

    Mutation

    sgRNA architecture from Amrani et al. Genome Biol…

    Promoter

    U6

    Available Since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRSFDuet-v2 RsTAL At4CL

    Plasmid

    #126524

    Purpose

    expresses TAL (Rhodobacter sphaeroides) and 4CL (Arabidopsis thaliana paralog 1) for PYP chromophore biosynthesis

    Depositor

    Andrew Woolley

    Article

    Brechun et al Biochemistry. 2019 Jun 11;58(23):2682-26

    Inserts

    tyrosine ammonia lyase
    p-coumaroyl-CoA ligase (4CL1 Mustard Weed)
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEN_TT 3xFLAG ID2

    Plasmid

    #98390

    Purpose

    Gateway entry vector for inducible expression of human ID2 with N-terminal 3xFLAG tag

    Depositor

    Kevin Janes

    Article

    Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

    Insert

    ID2 (ID2 Human)
    

    Use

    Entry vector for gateway cloning

    Tags

    3xFLAG

    Promoter

    TRE tight promoter

    Available Since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. NKP66 pEGFP-N2 (1176)

    Plasmid

    #62039

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Korfali et al PLoS One. 2011 Apr 14;6(4):e18762. doi:

    Insert

    MARCHV (MARCHF5 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAP03-sp44/p21

    Plasmid

    #120232

    Purpose

    Carries bidirectional promoter cassette containing sp44 and p21 promoters and apramycin resistance gene flanked by two FRT sites that can be amplified with homology sequence for refactoring.

    Depositor

    Bradley Moore

    Article

    Bauman et al Cell Chem Biol. 2019 Feb 15. pii: S2451-

    Insert

    bidirectional promoter cassette containing sp44 and p21 promoters and apramycin resistance gene flanked by two FRT sites
    

    Use

    Synthetic Biology

    Mutation

    aac(3)IV (apramycin resistance gene)

    Available Since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pENTR221-YAP1 CA

    Plasmid

    #79502

    Purpose

    MultiSite Gateway entry clones, second fragment (attL5, attL2) Human YAP1 S127A mutant CDS

    Depositor

    Peter Jon Nelson

    Article

    Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

    Insert

    YAP1 (YAP1 Human)
    

    Use

    Multisite gateway entry clone

    Tags

    3XFLAG

    Mutation

    S127A

    Promoter

    no Promoter

    Available Since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET-Sac-Abeta(MC1-42)

    Plasmid

    #127151

    Purpose

    Expresses N-terminal cysteine Abeta(1-42)

    Depositor

    James Nowick

    Article

    Zhang et al Biochemistry. 2021 Apr 20;60(15):1191-12

    Insert

    Abeta(MC1-42) (APP Human)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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