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pBLO 62.5

Plasmid

#123124

Purpose

Human expression vector for planctomyces CasX and sgRNA

Depositor

Jennifer Doudna, Benjamin Oakes

Article

Liu et al Nature. 2019 Feb 4. pii: 10.1038/s41586-

Inserts

CasX protein
CasX sgRNA

Use

CRISPR

Expression

Mammalian

Available Since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

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pBLO 62.4

Plasmid

#123123

Purpose

Human expression vector for deltaproteobacteria CasX and sgRNA

Depositor

Jennifer Doudna, Benjamin Oakes

Article

Liu et al Nature. 2019 Feb 4. pii: 10.1038/s41586-

Inserts

CasX protein
CasX sgRNA

Use

CRISPR

Expression

Mammalian

Available Since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

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G1333 DddAtox-C–dSpCas9

Plasmid

#157834

Purpose

expresses split DddAtox-Cas9 construct in mammalian cells

Depositor

David Liu

Article

Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

Insert

bpNLS–G1333 DddAtox-C–dSpCas9–UGI–UGI–bpNLS

Expression

Mammalian

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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IMPT-10316:GABR1

Plasmid

#157850

Purpose

HA-TEV-VFT-TMD-CC-PreX-eGFP in pFastBac1 for Sf9 expression

Depositor

Vadim Cherezov, Raymond Stevens

Article

Shaye et al Nature. 2020 Jun 17. pii: 10.1038/s41586

Insert

GABR1 (GABBR1 Human)

Expression

Insect

Promoter

Polyhedrin

Available Since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pDF0237 pCMV - dead huDisCas7-11 - NES - huADAR2

Plasmid

#172510

Purpose

Encodes human codon optimized catalytically dead DisCas7-11-NES-huADAR2 fusion protein alone for expression in mammalian cells

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

pCMV - dead huDisCas7-11 - NES - huADAR2

Expression

Mammalian

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pET28-His-MBP-NLS-RfxCas13d-NLS-HA

Plasmid

#171586

Purpose

Bacterial expression of NLS-RfxCas13d-NLS-HA

Depositor

Neville Sanjana

Article

Mendez-Mancilla et al Cell Chem Biol. 2022 Feb 17;29(2):321-32

Insert

His-MBP-NLS-RfxCas13d-NLS-HA

Tags

HA

Expression

Bacterial

Available Since

Oct. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pET 2C-T10-dCasX

Plasmid

#126418

Purpose

E. coli expression vector for inactive casX (triple mutations)--Used for CasX protein purification

Depositor

Jennifer Doudna

Article

Liu et al Nature. 2019 Feb 4. pii: 10.1038/s41586-

Insert

DpbCasX

Use

CRISPR

Tags

His-MBP

Expression

Bacterial

Available Since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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pCAGGS-Flag-hsDicer (D1320A/D1709A)

Plasmid

#41585

Depositor

Phil Sharp

Article

Gurtan et al RNA. 2012 Jun;18(6):1116-22. Epub 2012 A

Insert

hsDicer (DICER1 Human)

Tags

Flag

Expression

Mammalian

Mutation

D1320A/D1709A

Promoter

CMV

Available Since

Feb. 22, 2013

Availability

Academic Institutions and Nonprofits only
pCMV6-AN-HA_aa 1-420 POLI

Plasmid

#201582

Purpose

Expresses the catalytic domain (aa 1-420) of human DNA polymerase iota with a HA tag in mammalian cells

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

DNA polymerase iota (POLI Human)

Tags

HA

Expression

Mammalian

Mutation

Expresses amino acids 1-420 of DNA polymerase iota

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pDF0250 DisCas7-11 full locus with Golden gate acceptor site for spacer

Plasmid

#172501

Purpose

Encodes the DisCas7-11 full locus along with a DR and golden gate site for cloning spacers

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

DisCas7-11 full locus with Golden gate acceptor site for spacer

Expression

Bacterial

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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