We narrowed to 9,586 results for: Coli
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Plasmid#119834PurposeProduction of recombinant iGluSnFR S72T ultrafast variant, a single-wavelength glutamate indicator, in E. coliDepositorInsertiGluu
TagsGSTExpressionBacterialMutationS72TPromoterT7Available SinceApril 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pM9-BetaGal-8C
Plasmid#172489PurposePlasmid encoding E. coli BetaGalactosidase with an N-terminal M9 nuclear localization sequenceDepositorInsertM9-BetaGal-8C
Tags6xHis and M9 nuclear localization sequence (from …ExpressionBacterialMutationBetaGal has two cysteine mutations (C77A, C1022S)…PromoterT7Available SinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
PatoL-OG241
Plasmid#72841PurposeInduced by acetoacetate to express GFP using atoSC two-component system in E. coli. Contains the atoDAEB promoter (Pato) with a strong RBS, Bba_B0034 (iGEM). OG241 backbone.DepositorInsertPatoL
ExpressionBacterialPromoterPatoLAvailable SinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET15b/bPanK
Plasmid#73619PurposeProduces Escherichia coli pantothenate kinase, with N-terminal His6 tag (H6PanK)DepositorInsertpantothenate kinase (coaA Escherichia coli)
TagsHis6ExpressionBacterialMutationrelative to MG1655 sequence, changes glutamate-5 …PromoterT7Available SinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEB2_mScarletI_mVenusNB-MrkHR113A
Plasmid#182292Purposeexpresses c-di-GMP mutant biosensor in E. coliDepositorInsertmVenusNB-MrkHR113A
TagsmVenusNB internal fusion to MrkH with a R113A poi…ExpressionBacterialMutationchanged arginine 113 to alanine, MrkH numbering …PromoterproCAvailable SinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCarCBADE_ProB(I69E)A
Plasmid#117756PurposeProB(I69E)A and codon optimized CarCBADE enzymes (in that order) in pBbaA5k backboneDepositorInsertGlutamate kinase (ProA), mutant pyrroline-5-carboxylate synthase (ProBI69E) from E. coli and codon optimised CarCBADE operon
ExpressionBacterialPromoterPLac(UV5)Available SinceDec. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pK1E-RNAP
Plasmid#48144PurposeShuttle vector E. coli/B.meg.; encoding the RNA polymerase of the phage K1E under control of the xylose inducible promoter PxylADepositorInsertrna polymerase K1E
UseSynthetic BiologyExpressionBacterialAvailable SinceOct. 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET41a iGluSnFR
Plasmid#119828PurposeProduction of recombinant iGluSnFR, a single-wavelength glutamate indicator, in E. coliDepositorInsertiGluSnFR
TagsGSTExpressionBacterialPromoterT7Available SinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet1-His-TEV-OTC_Ub
Plasmid#172134PurposeScaffold design for studying ubiquitin interacting proteins by cryo-EM.DepositorInsertE. coli ornithine transcarbamylase (OTC), fused to human ubiquitin
Tags6xHis-TEVExpressionBacterialPromoterT7Available SinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET41a iGluh
Plasmid#119830PurposeProduction of recombinant iGluSnFR E25A high affinity variant, a single-wavelength glutamate indicator, in E. coliDepositorInsertiGluh
TagsGSTExpressionBacterialMutationE25APromoterT7Available SinceAug. 22, 2024AvailabilityAcademic Institutions and Nonprofits only