We narrowed to 6,927 results for: 11

1. CD4-C-BFP HDRT Source (pTR 176)

   Plasmid

   #112020

   Purpose

   DNA sequence source for amplifying an HDR template to tag endogenous human CD4 gene with BFP

   Depositor

   Alexander Marson

   Article

   Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

   Insert

   CD4-BFP HDRT (CD4 Human)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pWZL Sox2 R74P

   Plasmid

   #26355

   Depositor

   Matthew Meyerson

   Article

   Bass et al Nat Genet. 2009 Nov . 41(11):1238-42.

   Insert

   Sox2 (SOX2 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   R74P

   Promoter

   Available since

   Sept. 22, 2010

   Availability

   Academic Institutions and Nonprofits only

3. CD4-C-mCherry HDRT Source (pTR 175)

   Plasmid

   #112019

   Purpose

   DNA sequence source for amplifying an HDR template to tag endogenous human CD4 gene with mCherry

   Depositor

   Alexander Marson

   Article

   Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

   Insert

   CD4-mCherry HDRT (CD4 Human)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pRGEB32

   Plasmid

   #63142

   Purpose

   Express sgRNA/PTG with rice snoRNA U3 promoter and Cas9 with rice ubiquitin promoter for Agrobacterium-mediated transformation.

   Depositor

   Yinong Yang

   Article

   Xie et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Rice snoRNA U3 promoter for gRNA/PTG expression a…

   Available since

   April 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA GFP10-ZIP-GFP11

   Plasmid

   #182243

   Purpose

   Tripartite split-GFP mammalian. Express leucine zipper with GFP10 and GFP11 (self-assembing with GFP1-9)

   Depositor

   Stéphanie Cabantous

   Article

   Cabantous et al Sci Rep. 2013 Oct 4;3:2854. doi: 10.1038

   Insert

   GFP10-ZIP-11
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Stat3 pcDNA3

   Plasmid

   #8706

   Purpose

   See Depositor Comments below for variant information.

   Depositor

   Jim Darnell

   Article

   Zhong et al Proc Natl Acad Sci U S A 1994 May 24;91(

   Insert

   Stat3 (Stat3 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   * see comments

   Promoter

   Available since

   Sept. 26, 2005

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. LentiCRISPR-V2_mMSH2

   Plasmid

   #186156

   Purpose

   sgRNA targeting murine Msh2 gene

   Depositor

   David McFadden

   Article

   Povedano et al Cell Chem Biol. 2019 Sep 19;26(9):1315-1

   Insert

   Msh2_gRNA (Msh2 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   July 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAV-U6+27-Tornado-RhoBAST

   Plasmid

   #196341

   Purpose

   Overexpression of circular RhoBAST

   Depositor

   Murat Sunbul

   Article

   Sunbul et al Nat Biotechnol. 2021 Jun;39(6):686-690.

   Insert

   Tornado-RhoBAST
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6+27

   Available since

   Feb. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pTRE-Slow-FT

   Plasmid

   #31915

   Depositor

   Vladislav Verkhusha

   Article

   Subach et al Nat Chem Biol. 2009 Feb;5(2):118-26. Epu

   Insert

   Slow-FT
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   M18V/E30V/K69R/L84W/A179V compared to mCherry (bu…

   Promoter

   PTight Tet-responsive PminCMVd

   Available since

   Dec. 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pTRE-Medium-FT

    Plasmid

    #31914

    Depositor

    Vladislav Verkhusha

    Article

    Subach et al Nat Chem Biol. 2009 Feb;5(2):118-26. Epu

    Insert

    Medium-FT
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    M18L/N23D/T43S/K69R/L84W/M152I/Q194L/L205M/Y221C/…

    Promoter

    PTight Tet-responsive PminCMVd

    Available since

    Sept. 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGY037 BSMV-gamma-AmCyan

    Plasmid

    #234818

    Purpose

    To express flourescent protein AmCyan from the gamma genome of Barley Stripe Mosaic Virus Vector (T-DNA)

    Depositor

    Daniel Voytas

    Article

    Baysal et al Plant J. 2025 Jan;121(2):e17196. doi: 10

    Insert

    AmCyan
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCB094 FoMV-sgLw

    Plasmid

    #234816

    Purpose

    To express guide gRNA from Foxtail Mosaic Virus Vector targeting SbLw (T-DNA)

    Depositor

    Daniel Voytas

    Article

    Baysal et al Plant J. 2025 Jan;121(2):e17196. doi: 10

    Insert

    gRNA targeting SbLw
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pHR-TRE3G-dCas9-EGFP-HaloTag

    Plasmid

    #169451

    Purpose

    expression of dCas9-EGFP-HaloTag

    Depositor

    Stanley Qi

    Article

    Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

    Insert

    dCas9-EGFP-HaloTag
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. CD4-MKII-hLOV1-TEVcs(ENLYFQ/M)- Gal4

    Plasmid

    #163026

    Purpose

    FLiCRE TF for HEK293T expression

    Depositor

    Alice Ting

    Article

    Kim et al Cell. 2020 Dec 23;183(7):2003-2019.e16.

    Insert

    CD4-MKII-hLOV1-TEVcs-Gal4
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTRE-Fast-FT

    Plasmid

    #31913

    Depositor

    Vladislav Verkhusha

    Article

    Subach et al Nat Chem Biol. 2009 Feb;5(2):118-26. Epu

    Insert

    Fast-FT
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    M18V/E34K/K69R/L84W/S149T/A224S compared to mCher…

    Promoter

    PTight Tet-responsive PminCMVd

    Available since

    Sept. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3.1 3xFlag IFIT1

    Plasmid

    #53554

    Purpose

    mammalian expression of IFIT1

    Depositor

    Kathleen Collins

    Article

    Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

    Insert

    IFIT1 (IFIT1 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFast-FT-N1

    Plasmid

    #31910

    Depositor

    Vladislav Verkhusha

    Article

    Subach et al Nat Chem Biol. 2009 Feb;5(2):118-26. Epu

    Insert

    Fast-FT
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    M18V/E34K/K69R/L84W/S149T/A224S compared to mCher…

    Promoter

    CMV

    Available since

    Sept. 12, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTM1_NP_ZEBOV

    Plasmid

    #69121

    Purpose

    Encodes for Ebola Virus Zaire NP under control of a T7 RNA polymerase promoter

    Depositor

    Elke Mühlberger

    Article

    Trunschke et al Virology. 2013 Jul 5;441(2):135-45. doi:

    Insert

    NP ZEBOV (NP )
    

    Use

    T7

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET21a - NR

    Plasmid

    #89625

    Purpose

    Expresses recombinant 6HIS tagged T. cruzi nitrate reductase in E. coli upon IPTG induction

    Depositor

    Judy Lieberman

    Article

    Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

    Insert

    Nitrate reductase
    

    Use

    Tags

    6HIS and T7 tag (gene 10 leader)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pT7-EGFP-C1-HsXRN1_K

    Plasmid

    #146784

    Purpose

    Mammalian Expression of HsXRN1

    Depositor

    Elisa Izaurralde

    Article

    Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

    Insert

    HsXRN1 (XRN1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pSlow-FT-N1

    Plasmid

    #31912

    Depositor

    Vladislav Verkhusha

    Article

    Subach et al Nat Chem Biol. 2009 Feb;5(2):118-26. Epu

    Insert

    Slow-FT
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    M18V/E30V/K69R/L84W/A179V compared to mCherry (bu…

    Promoter

    CMV

    Available since

    Sept. 12, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pTara:500

    Plasmid

    #60717

    Purpose

    Expresses T7 RNAP from the arabinose inducible promoter PBAD with a RBS strength of 500 from the Salis RBS calculator. Also includes a constitutive araC ORF

    Depositor

    Matthew Bennett

    Article

    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

    Insert

    T7 RNAP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PBAD

    Available since

    Nov. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. EGFP-Lyn11C32S-PIP4K2A

    Plasmid

    #202748

    Purpose

    weakly plasma membrane targeted fluorescent protein-tagged enzyme

    Depositor

    Gerry Hammond

    Article

    Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

    Insert

    EGFP:LYN (1-11) (C3S):PIP4K2A (PIP4K2A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAT253

    Plasmid

    #40943

    Depositor

    Angela Taddei

    Article

    Dubarry et al Genes Dev. 2011 Jul 1;25(13):1365-70.

    Insert

    GFP-LacI**
    

    Use

    Tags

    GFP(S65T, V163A) and SV40 NLS

    Expression

    Yeast

    Mutation

    LacI**/LacI2G(4 mutations) (see comment below); l…

    Promoter

    His3 promoter fragment

    Available since

    Feb. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pSUB7

    Plasmid

    #169698

    Purpose

    Template plasmid for generating C-terminal 6-His epitope tag fusions to bacterial genes by "Lambda red" recombineering.

    Depositor

    Nara Figueroa

    Article

    Uzzau et al Proc Natl Acad Sci U S A. 2001 Dec 18;98

    Insert

    6xHis epitope tag adjacent to FRT-flanked aph (KanR) gene
    

    Use

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pSU315

    Plasmid

    #169697

    Purpose

    Template plasmid for generating C-terminal hemagglutinin (1xHA) epitope tag fusions to bacterial genes by "Lambda red" recombineering.

    Depositor

    Nara Figueroa

    Article

    Uzzau et al Proc Natl Acad Sci U S A. 2001 Dec 18;98

    Insert

    1xHA epitope tag adjacent to FRT-flanked aph (KanR) gene
    

    Use

    Tags

    1xHA tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pST-2K

    Plasmid

    #44564

    Purpose

    for ectopic constitutive expression of two mycobacterial proteins in one vector

    Depositor

    Vinay Nandicoori

    Article

    Parikh et al Appl Environ Microbiol. 2013 Mar;79(5):1

    Type

    Empty backbone

    Use

    E.coli-mycobacteria shuttle vector

    Tags

    Flag tag and His tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCS2+cPLA2-mKate2

    Plasmid

    #162068

    Purpose

    Used to provide a steady-state readout of cPLA2 activation in live cells. Amino-terminally fused to mKate2 for red fluorescence.

    Depositor

    Philipp Niethammer

    Article

    Enyedi et al Nat Cell Biol. 2013 Sep;15(9):1123-30. d

    Insert

    cytosolic phospholipase a2 (pla2g4aa Zebrafish)
    

    Use

    Avian, xenopus, zebrafish

    Tags

    mKate2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMBA334

    Plasmid

    #214746

    Purpose

    High copy thyA-infA plasmid containing gfp and no antibiotic resistance gene (ARG)

    Depositor

    Tae Seok Moon

    Article

    Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

    Insert

    BBa_J23119-RiboJ-RBS(21992)-gfpmut3
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pHR-TRE3G-dCas9-HaloTag

    Plasmid

    #169448

    Purpose

    expression of dCas9-HaloTag

    Depositor

    Stanley Qi

    Article

    Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

    Insert

    dCas9-HaloTag
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge