We narrowed to 70,757 results for: LAS

1. pKP999

   Plasmid

   #160816

   Purpose

   Expression of ExbD protein

   Depositor

   Kathleen Postle

   Article

   Ollis et al Mol Microbiol. 2009 Aug;73(3):466-81. do

   Insert

   exbD
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. LP102: pMAGIC (R4-R3) NLS-Sp Cas9-NLS

   Plasmid

   #132927

   Purpose

   pMAGIC R4-R3 entry plasmid, contains 2xNLS SpCas9 (nuclease active) for 3 or 4-component MultiSite Gateway Pro assembly.

   Depositor

   Christopher Newgard

   Insert

   humanized SpCas9
   

   Use

   CRISPR and Synthetic Biology; Pmagic gateway entr…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDK365

   Plasmid

   #134195

   Purpose

   Retroviral plasmid with SKAP insert

   Depositor

   Iain Cheeseman

   Insert

   SKAP Alternate Start- LAP (with G5 hardeneded) (KNSTRN Human)
   

   Use

   Retroviral

   Tags

   LAP (GFP)

   Expression

   Mammalian

   Mutation

   Alternate Start, G5 siRNA hardened

   Promoter

   Available since

   Feb. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSBbi-Pur-Lyn-AktAR2-EV-D

   Plasmid

   #125200

   Purpose

   SB-transposon plasmid for stable expression of unphosphorylatable "dead" version of lipid raft-targeted Lyn-AktAR2-EV variant of FRET-based AKT activity reporter

   Depositor

   Eric Davis

   Insert

   Lyn-AktAR2-EV-D
   

   Use

   Transposon

   Tags

   EV linker, cpVenus[E172] and Lyn tag (GCIKSKRKD),…

   Expression

   Mammalian

   Mutation

   FoxO1 Thr-24 changed to Val rendering this inacti…

   Promoter

   EF1a/RPBSA

   Available since

   May 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMSX2.1.0-gDNA

   Plasmid

   #113793

   Purpose

   CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor MSX2

   Depositor

   Kevin White

   Insert

   MSX2 (MSX2 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTOX2.1.0-gDNA

   Plasmid

   #113787

   Purpose

   CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor TOX2

   Depositor

   Kevin White

   Insert

   TOX2 (TOX2 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTSHZ2.1.0-gDNA

   Plasmid

   #113778

   Purpose

   CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor TSHZ2

   Depositor

   Kevin White

   Insert

   TSHZ2 (TSHZ2 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pZNF462.1.0-gDNA

   Plasmid

   #112476

   Purpose

   CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZNF462

   Depositor

   Kevin White

   Insert

   ZNF462 (ZNF462 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pZNF197.1.0-gDNA

   Plasmid

   #112453

   Purpose

   CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZNF197

   Depositor

   Kevin White

   Insert

   ZNF197 (ZNF197 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMYNN.1.0-gDNA

    Plasmid

    #112450

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor MYNN

    Depositor

    Kevin White

    Insert

    MYNN (MYNN Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pZNF639.1.0-gDNA

    Plasmid

    #112441

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZNF639

    Depositor

    Kevin White

    Insert

    ZNF639 (ZNF639 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pZNF23.1.0-gDNA

    Plasmid

    #112421

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZNF23

    Depositor

    Kevin White

    Insert

    ZNF23 (ZNF23 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTBPL1.1.0-gDNA

    Plasmid

    #112412

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor TBPL1

    Depositor

    Kevin White

    Insert

    TBPL1 (TBPL1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pNR1D2.1.0-gDNA

    Plasmid

    #112410

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor NR1D2

    Depositor

    Kevin White

    Insert

    NR1D2 (NR1D2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pZNF354B.1.0-gDNA

    Plasmid

    #112405

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZNF354B

    Depositor

    Kevin White

    Insert

    ZNF354B (ZNF354B Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. WNK3 (WNK3B-c005)

    Plasmid

    #110262

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    WNK3 (WNK3 Human)
    

    Use

    Tags

    His-TEV

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin promoter

    Available since

    June 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. WNK3 (WNK3B-c001)

    Plasmid

    #110261

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    WNK3 (WNK3 Human)
    

    Use

    Tags

    His-TEV

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin promoter

    Available since

    June 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. WNK2 (WNK2A-c003)

    Plasmid

    #110250

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    WNK2 (WNK2 Human)
    

    Use

    Tags

    His-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7 - lacO

    Available since

    June 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLV-hUV6-sgRNA-dCas9-KRAB-TagBFP2 (identifier AAAA-0244)

    Plasmid

    #202553

    Purpose

    Nontargeting control vector with TagBFP2

    Depositor

    Frederic Meunier

    Article

    Joensuu et al EMBO J. 2023 Jul 3;42(13):e112095. doi:

    Insert

    Humanized dCas9-KRAB-TagBFP2 (tag blue fluorescent protein 2) (TRIM28 S. Pyogenes (dCas9), H. sapiens (KRAB), Entacmaea quadricolor (TagBFP2))
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2

    Expression

    Mutation

    Puromycin-resistance gene in the pLV hU6-sgRNA hU…

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-OVA

    Plasmid

    #64599

    Purpose

    Expression of chicken ovalbumin as model antigen for study of MHC class I and class II presentation

    Depositor

    Martin Zenke, Sandra Diebold

    Article

    Diebold et al Gene Ther. 2001 Mar;8(6):487-93.

    Insert

    Ovalbumin
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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21. ER-mRFP

    Plasmid

    #62236

    Purpose

    Plasmid expresses endoplasmic reticulum targeted mRFP in mammalian cells.

    Depositor

    Erik Snapp

    Article

    Snapp et al Proc Natl Acad Sci U S A. 2006 Apr 25;10

    Insert

    ER-mRFP
    

    Use

    Tags

    mRFP1

    Expression

    Mammalian

    Mutation

    Promoter

    cmv

    Available since

    March 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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22. pAAV-CMV-FLEX-SaCas9-U6-sgRosa26

    Plasmid

    #159914

    Purpose

    Mutagenesis of Rosa26 with SauCas9

    Depositor

    Larry Zweifel

    Insert

    Rosa26 gRNA (Gt(ROSA)26Sor Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. AiP14801 - pAAV-AiE0390m_3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN4801)

    Plasmid

    #208163

    Purpose

    AiE0390m_3xC2 is an optimized enhancer sequence designed to drive AAV-mediated Cre recombination in brain astrocytes

    Depositor

    Jonathan Ting, The Allen Institute for Brain Science

    Insert

    iCreR297T
    

    Use

    AAV

    Tags

    Expression

    Mutation

    NA

    Promoter

    Beta Globin minimal promoter

    Available since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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24. AiP13999 - pAAV-AiE0387m-minBG-iCre(R297T)-BGHpA (Alias: CN3999)

    Plasmid

    #208166

    Purpose

    AiE0387m is an enhancer sequence designed to drive AAV-mediated Cre recombination in brain astrocytes

    Depositor

    Jonathan Ting, The Allen Institute for Brain Science

    Insert

    iCreR297T
    

    Use

    AAV

    Tags

    Expression

    Mutation

    NA

    Promoter

    Beta Globin minimal promoter

    Available since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pZA16mflon

    Plasmid

    #75439

    Purpose

    Plasmid for arabinose-induced expression of mf-lon. Note that this plasmid contains the original mf-lon cassette that is not codon optimized for expression in E. coli.

    Depositor

    James Collins

    Article

    Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

    Insert

    mf-lon
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    Oct. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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26. S96 ELP

    Plasmid

    #68393

    Purpose

    Elastin-like polypeptide (VPGxG) with 96 repeats of Serine guest residue

    Depositor

    Andrew MacKay

    Article

    Shah et al Protein Sci. 2012 Jun;21(6):743-50. doi:

    Insert

    S96 ELP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pESC-URA (V5 and HA)

    Plasmid

    #222485

    Purpose

    Co-expression of two genes in yeast with V5 and HA tags and URA3 selection

    Depositor

    Kiyofumi Yamada, Tetsuo Matsuzaki

    Article

    Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

    Type

    Empty backbone

    Use

    Tags

    HA and V5

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pESC-ADE (V5 and HA)

    Plasmid

    #222489

    Purpose

    Co-expression of two genes in yeast with V5 and HA tags and ADE2 selection

    Depositor

    Kiyofumi Yamada, Tetsuo Matsuzaki

    Article

    Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

    Type

    Empty backbone

    Use

    Tags

    HA and V5

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pJYDNg

    Plasmid

    #162452

    Purpose

    Yeast display plasmid intended for expression of N-terminal fusions with Aga2p yeast agglutinin. The plasmid is carrying the eUnaG2 fluorescent protein and long 2G linker.

    Depositor

    Gideon Schreiber

    Article

    Zahradník et al bioRxiv Preprint 2020

    Type

    Empty backbone

    Use

    Shuttle vector bacteria/yeast

    Tags

    Ha and myc and eUnaG2 fluorescent protein

    Expression

    Mutation

    Promoter

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET28-6His-SUMO-Nsp10

    Plasmid

    #154959

    Purpose

    Expresses Nsp10 from SARS-CoV-2 with a N-terminal SUMO-protease cleavable 6His-SUMO tag in E. coli

    Depositor

    Justin Kollman

    Insert

    6His-SUMO-Nsp10 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    6His-Thrombin-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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