We narrowed to 9,187 results for: Pol;

1. p1A CbbL-

   Plasmid

   #162693

   Purpose

   p1A negative control expressing inactive CbbLS (carbamylated K194M mutation) and Prk

   Depositor

   David Savage

   Article

   Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

   Insert

   H. neapolitanus CbbLS and S. elongatus Prk
   

   Tags

   N terminal 6x His tag on PRK

   Expression

   Bacterial

   Mutation

   CbbL K194M (inactive rubisco)

   Promoter

   PLtet0-1 promoter

   Available Since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNG1304

   Plasmid

   #162488

   Purpose

   Expresses HelD K239A from Bacillus subtillis

   Depositor

   Peter Lewis

   Article

   Newing et al Nat Commun. 2020 Dec 18;11(1):6420. doi:

   Insert

   HelD (helD Bacillus subtillis 168)
   

   Tags

   6His-V5

   Expression

   Bacterial

   Mutation

   Changed Lysine 239 to Alanine (K239A)

   Available Since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX330_gRNA_IG-DMR-1

   Plasmid

   #159931

   Purpose

   Specific gRNA against mouse IG-DMR cloned in the pX330 backbone (Addgene Number 42230). Deletion of IG-DMR in mouse ESC.

   Depositor

   Anton Wutz

   Article

   Aizawa et al PLoS One. 2020 Sep 10;15(9):e0233072. do

   Insert

   gRNA mouse IG-DMR
   

   Use

   CRISPR and Mouse Targeting

   Expression

   Mammalian

   Promoter

   U6, CBh

   Available Since

   Nov. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX330_gRNA_IG-DMR-2

   Plasmid

   #159932

   Purpose

   Specific gRNA against mouse IG-DMR cloned in the pX330 backbone (Addgene Number 42230). Deletion of IG-DMR in mouse ESC.

   Depositor

   Anton Wutz

   Article

   Aizawa et al PLoS One. 2020 Sep 10;15(9):e0233072. do

   Insert

   gRNA mouse IG-DMR
   

   Use

   CRISPR and Mouse Targeting

   Expression

   Mammalian

   Promoter

   U6, CBh

   Available Since

   Nov. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGL3-Basic-ZC3H12D_PromoterWT

   Plasmid

   #153065

   Purpose

   ZC3H12D promoter region cloned upstream of luciferase reporter gene

   Depositor

   Silvia Monticelli

   Article

   Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi

   Insert

   ZC3H12D promoter region (ZC3H12D Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Promoter

   None

   Available Since

   Sept. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKM5

   Plasmid

   #154166

   Purpose

   Aedes aegypti Rel2 (long isoform) expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virus.

   Depositor

   Kevin Maringer

   Article

   Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

   Insert

   Rel2 (long isoform)
   

   Tags

   FLAG

   Expression

   Insect

   Promoter

   IE1 promoter and hr5 enhancer from Autographa cal…

   Available Since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKM28

   Plasmid

   #154165

   Purpose

   Aedes aegypti Rel1A expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virus

   Depositor

   Kevin Maringer

   Article

   Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

   Insert

   Rel1A
   

   Tags

   FLAG

   Expression

   Insect

   Promoter

   IE1 promoter and hr5 enhancer from Autographa cal…

   Available Since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. EGFP-Mm_Plk5(D168A)

   Plasmid

   #136318

   Purpose

   Mammalian expression of mouse Plk5-KD (D168A) with N-terminal EGFP

   Depositor

   Guillermo de Cárcer, Marcos Malumbres

   Article

   de Carcer et al Mol Cell Biol. 2011 Mar;31(6):1225-39. d

   Insert

   PLK5 (Plk5 Mouse)
   

   Expression

   Mammalian

   Mutation

   D168A

   Available Since

   Aug. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. EGFP-Hu_Plk5(W207F)

   Plasmid

   #136320

   Purpose

   Mammalian expression of human Plk5 W207F with N-terminal EGFP

   Depositor

   Guillermo de Cárcer, Marcos Malumbres

   Article

   de Carcer et al Mol Cell Biol. 2011 Mar;31(6):1225-39. d

   Insert

   PLK5 (PLK5 Human)
   

   Expression

   Mammalian

   Mutation

   W207F

   Available Since

   Aug. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. Lenti-XYLT2-sgRNA

    Plasmid

    #154862

    Purpose

    Lentiviral expression of Cas9 and sgRNA targeting XYLT2

    Depositor

    Yihong Ye

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2020 May 19;11

    Insert

    XYLT2 sgRNA (XYLT2 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    July 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDF11-SFPQ(214-535)

    Plasmid

    #135439

    Purpose

    Expresses SFPQ 214-535 with TEV-cleavable hexahistidine tag

    Depositor

    Charles Bond

    Article

    Lee et al Nucleic Acids Res. 2015 Apr 20;43(7):382

    Insert

    splicing factor proline and glutamine rich (SFPQ Human)
    

    Tags

    Hexahistidine

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET14b-dabAB2 D353A

    Plasmid

    #133016

    Purpose

    pET14b carrying the dabA2 gene (Uniprot: D0KWS7) with a D353A mutation fused to a c-terminal strep tag and the dabB2 gene (Uniprot: D0KWS8) with a c-terminal sfGFP V206K fusion and 6xHis tag

    Depositor

    David Savage

    Article

    Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

    Inserts

    dabB2
    dabA2
    

    Tags

    6xHis, StrepII tag, and sfGFP

    Expression

    Bacterial

    Mutation

    D353A

    Promoter

    t7

    Available Since

    Nov. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET14b-dabAB2 C351A

    Plasmid

    #133014

    Purpose

    pET14b carrying the dabA2 gene (Uniprot: D0KWS7)  with a C351A mutation fused to a c-terminal strep tag and the dabB2 gene (Uniprot: D0KWS8) with a c-terminal sfGFP V206K fusion and 6xHis tag

    Depositor

    David Savage

    Article

    Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

    Inserts

    dabB2
    dabA2
    

    Tags

    6xHis, StrepII tag, and sfGFP

    Expression

    Bacterial

    Mutation

    C351A

    Promoter

    t7

    Available Since

    Nov. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET15b-GreBdm-D41N

    Plasmid

    #129691

    Purpose

    express E. coli GreB E82C/C68S/D41N

    Depositor

    Jeff Gelles

    Article

    Tetone et al Proc Natl Acad Sci U S A. 2017 Feb 14;11

    Insert

    GreB E82C C68S D41N (greB E. coli)
    

    Tags

    his6

    Expression

    Bacterial

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMH-HT_CtCHAD-H253A-R256A-R260A

    Plasmid

    #127788

    Purpose

    Expresses a CtCHAD mutant protein in E. coli cells

    Depositor

    Michael Hothorn

    Article

    Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

    Insert

    CtCHAD (CT0884 Chlorobium tepidum)
    

    Tags

    6xHis tag + TEV cleavage site

    Expression

    Bacterial

    Mutation

    Residues 208-522 (CHAD domain), mutations in His2…

    Available Since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMH-HT_CtCHAD-H253A-R256A-R260A-R296A

    Plasmid

    #127789

    Purpose

    Expresses a CtCHAD mutant protein in E. coli cells

    Depositor

    Michael Hothorn

    Article

    Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

    Insert

    CtCHAD (CT0884 Chlorobium tepidum)
    

    Tags

    6xHis tag + TEV cleavage site

    Expression

    Bacterial

    Mutation

    Residues 208-522 (CHAD domain), mutations in His2…

    Available Since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMH-HT_CtCHAD-R385A-K386A-K389A-K390A

    Plasmid

    #127792

    Purpose

    Expresses a CtCHAD mutant protein in E. coli cells

    Depositor

    Michael Hothorn

    Article

    Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

    Insert

    CtCHAD (CT0884 Chlorobium tepidum)
    

    Tags

    6xHis tag + TEV cleavage site

    Expression

    Bacterial

    Mutation

    Residues 208-522 (CHAD domain), mutations in Arg3…

    Available Since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. PCRII apoA-Ib

    Plasmid

    #121896

    Purpose

    Plasmid for making zebrafish apoA-Ib in situ probe

    Depositor

    Steven Farber

    Article

    Otis et al Dis Model Mech. 2015 Mar;8(3):295-309. d

    Insert

    ApoA1b (apoa1b Zebrafish)
    

    Available Since

    May 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. PCRII apoBb.2

    Plasmid

    #121899

    Purpose

    Plasmid for making zebrafish apoBb.2 in situ probe

    Depositor

    Steven Farber

    Article

    Otis et al Dis Model Mech. 2015 Mar;8(3):295-309. d

    Insert

    ApoBb.2 (apobb.2 Zebrafish)
    

    Use

    Unspecified

    Available Since

    May 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. PCRII apoA-IVb.3

    Plasmid

    #121905

    Purpose

    Plasmid for making zebrafish apoA-IVb.3 in situ probe

    Depositor

    Steven Farber

    Article

    Otis et al Dis Model Mech. 2015 Mar;8(3):295-309. d

    Insert

    apoa4b.3 (apoa4b.3 Zebrafish)
    

    Use

    Unspecified

    Available Since

    May 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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21. PCRII apoA-IVa

    Plasmid

    #121902

    Purpose

    Plasmid for making zebrafish apoA-IVa in situ probe

    Depositor

    Steven Farber

    Article

    Otis et al Dis Model Mech. 2015 Mar;8(3):295-309. d

    Insert

    ApoA4a (apoa4a Zebrafish)
    

    Use

    Unspecified

    Available Since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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22. PCRII apoA-Ia

    Plasmid

    #121895

    Purpose

    Plasmid for making zebrafish apoA-Ia in situ probe

    Depositor

    Steven Farber

    Article

    Otis et al Dis Model Mech. 2015 Mar;8(3):295-309. d

    Insert

    apoA-Ia (apoa1a Zebrafish)
    

    Available Since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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23. pRK5 Flag RagC (D181N)

    Plasmid

    #112752

    Purpose

    express Flag-RagC (D181N mutant)

    Depositor

    Roberto Zoncu

    Article

    Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

    Insert

    Flag-RagC (D181N) (RRAGC Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    181N

    Available Since

    Oct. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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24. pEGFP-N1 Dynamin1 P294A

    Plasmid

    #112102

    Purpose

    Mammalian expression of GFP-tagged Dynamin protein.

    Depositor

    Justin Taraska

    Article

    Kong et al Nature. 2018 Aug;560(7717):258-262. doi:

    Insert

    Dynamin1 (DNM1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    P294A

    Promoter

    CMV

    Available Since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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25. p.UTA.2.0-GL-2-3 perfect copies

    Plasmid

    #82441

    Purpose

    Dual Fluorescent endogenous miRNA sensor Control insert. Three Perfect copies of complementary region of a siRNA control targeting firefly luciferase encoded by the pGL2

    Depositor

    Jens Gruber

    Article

    Lemus-Diaz et al Sci Rep. 2017 Mar 24;7:45197. doi: 10.10

    Insert

    Luciferase
    

    Mutation

    The complementary region for GL2 siRNA control wa…

    Available Since

    March 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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26. pEMS1962

    Plasmid

    #82572

    Purpose

    High copy number plasmid containing Ple303 MiniPromoter insert.

    Depositor

    Elizabeth Simpson

    Article

    Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

    Insert

    Ple303
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Available Since

    Sept. 14, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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27. pEMS1963

    Plasmid

    #82573

    Purpose

    High copy number plasmid containing Ple304 MiniPromoter insert.

    Depositor

    Elizabeth Simpson

    Article

    Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

    Insert

    Ple304
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Available Since

    Sept. 14, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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28. pEMS1961

    Plasmid

    #82571

    Purpose

    High copy number plasmid containing Ple302 MiniPromoter insert.

    Depositor

    Elizabeth Simpson

    Article

    Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

    Insert

    Ple302
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Available Since

    Sept. 14, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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29. pEMS1960

    Plasmid

    #82570

    Purpose

    High copy number plasmid containing Ple301 MiniPromoter insert.

    Depositor

    Elizabeth Simpson

    Article

    Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

    Insert

    Ple301
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Available Since

    Sept. 14, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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30. pEMS1957

    Plasmid

    #82568

    Purpose

    High copy number plasmid containing hs1218 MiniPromoter insert.

    Depositor

    Elizabeth Simpson

    Article

    Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

    Insert

    hshs1218
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Available Since

    Sept. 14, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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