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pCF826-sgCIDE-1_U6-sgRNA-EFS-Cas9-P2A-mCherry2

Plasmid

#211690

Purpose

U6-sgRNA-EFS-Cas9-P2A-mCherry2

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

SpyCas9 and sgCIDE-1 guide RNA vector

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pCF826-sgCIDE-2_U6-sgRNA-EFS-Cas9-P2A-mCherry2

Plasmid

#211691

Purpose

U6-sgRNA-EFS-Cas9-P2A-mCherry2

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

SpyCas9 and sgCIDE-2 guide RNA vector

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV_hsyn_HA_Flag-uMASS_LF

Plasmid

#209761

Purpose

AAV virus production for neuonal expression of uMASS (loss-of-function) under hSyn promoter

Depositor

Andre Berndt

Article

Rappleye et al ACS Sens. 2023 Nov 24;8(11):4233-4244. d

Insert

uMASS_LF

Use

AAV

Expression

Mammalian

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pCF826-recipient_U6-sgRNA-EFS-Cas9-P2A-mCherry2

Plasmid

#211688

Purpose

U6-sgRNA-EFS-Cas9-P2A-mCherry2

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

SpyCas9 and guide RNA recipient vector

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pCF827-sgNT-2_U6-sgRNA-EFS-Cas9-P2A-mNeonGreen

Plasmid

#211694

Purpose

U6-sgRNA-EFS-Cas9-P2A-mNeonGreen

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

SpyCas9 and sgNT-2 guide RNA vector

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-hsyn-dMASS_LF_WPRE

Plasmid

#209766

Purpose

AAV virus production for neuronal expression of dMASS_LF (loss-of-function) under hSyn promoter

Depositor

Andre Berndt

Article

Rappleye et al ACS Sens. 2023 Nov 24;8(11):4233-4244. d

Insert

dMASS_LF

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available Since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV_hsyn_HA-Flag-uMASS_1_WPRE

Plasmid

#209760

Purpose

AAV virus production for neuronal expression of uMASS_1 under hSyn promoter

Depositor

Andre Berndt

Article

Rappleye et al ACS Sens. 2023 Nov 24;8(11):4233-4244. d

Insert

uMASS_1

Use

AAV

Expression

Mammalian

Available Since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pAS_4xMmaPylT(UUA)_EF1_sfGFP150TAA

Plasmid

#174897

Purpose

ochre suppression reporter sfGFP150TAA expression, with MmaPylT(UUA) ochre suppressor tRNA cassette

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

sf GFP

Expression

Mammalian

Mutation

150TAA in sfGFP

Promoter

EF1

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pAS_4xMmaPylT(UCA)_EF1_sfGFP150TGA

Plasmid

#174898

Purpose

opal suppression reporter sfGFP150TGA expression, with MmaPylT(UCA) opal suppressor tRNA cassette

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

sfGFP

Expression

Mammalian

Mutation

150TGA in sfGFP

Promoter

EF1

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pTP-PCR C4

Plasmid

#189621

Purpose

Cloned Reduced Mitochondrial Genome of the diatom Thalassiosira pseudonana in yeast and bacteria

Depositor

Bogumil Karas

Article

Cochrane et al Biology (Basel). 2020 Oct 26;9(11). pii:

Insert

Reduced Mitochondrial Genome of Thalassiosira pseudonana

Use

Synthetic Biology; Diatom expression

Expression

Bacterial and Yeast

Mutation

Repeat region removed, and minor additional mutat…

Available Since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmNot2-del393-429-dsRNAres_U

Plasmid

#147712

Purpose

Insect Expression of DmNot2-del393-429-dsRNAres

Depositor

Elisa Izaurralde

Article

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

DmNot2-del393-429-dsRNAres (Rga Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmNot2-del429-453-dsRNAres_U

Plasmid

#147714

Purpose

Insect Expression of DmNot2-del429-453-dsRNAres

Depositor

Elisa Izaurralde

Article

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

DmNot2-del429-453-dsRNAres (Rga Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-lambdaN-HA-DmNot2-W556E-dsRNAres_U

Plasmid

#147717

Purpose

Insect Expression of DmNot2-W556E-dsRNAres

Depositor

Elisa Izaurralde

Article

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

DmNot2-W556E-dsRNAres (Rga Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-HsNot2-344-540_U

Plasmid

#147725

Purpose

Bacterial Expression of HsNot2-344-540

Depositor

Elisa Izaurralde

Article

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

HsNot2-344-540 (CNOT2 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-HsNot2_452-540_U

Plasmid

#147726

Purpose

Bacterial Expression of HsNot2_452-540

Depositor

Elisa Izaurralde

Article

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

HsNot2_452-540 (CNOT2 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-HsNot2_467-540_U

Plasmid

#147727

Purpose

Bacterial Expression of HsNot2_467-540

Depositor

Elisa Izaurralde

Article

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

HsNot2_467-540 (CNOT2 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-NpM-HsNot1iso1_1833-2361_U

Plasmid

#147728

Purpose

Bacterial Expression of HsNot1iso1_1833-2361

Depositor

Elisa Izaurralde

Article

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

HsNot1iso1_1833-2361 (CNOT1 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-NvH-HsNot1iso1_1565-2371_U

Plasmid

#147730

Purpose

Bacterial Expression of HsNot1iso1_1565-2371

Depositor

Elisa Izaurralde

Article

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

HsNot1iso1_1565-2371 (CNOT1 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-EGFP-DmNot2-del393-429-dsRNAres_U

Plasmid

#147697

Purpose

Insect Expression of DmNot2-del393-429-dsRNAres

Depositor

Elisa Izaurralde

Article

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

DmNot2-del393-429-dsRNAres (Rga Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-EGFP-DmNot2-del429-453-dsRNAres_U

Plasmid

#147699

Purpose

Insect Expression of DmNot2-del429-453-dsRNAres

Depositor

Elisa Izaurralde

Article

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

DmNot2-del429-453-dsRNAres (Rga Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge