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pIDTSmart-CMV-PLRS1

Plasmid

#217365

Purpose

Expression of previously developed E. coli leucyl aminoacyl-tRNA synthesis variant that is "polyspecific"

Depositor

Abhishek Chatterjee

Publication

Huang et al ACS Synth Biol. 2024 Jul 19;13(7):2141-2

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

leucyl-tRNA synthetase polyspecific mutant

Expression

Mammalian

Mutation

M40I, T252A, Y499I, Y527A, H529G

Promoter

CMV

Available since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pTWIN1-His6-Ssp-Htt2-90(A2C)-37Q

Plasmid

#114617

Purpose

Bacterial expression of Htt fragment containing amino acids 2-90, with A2C cysteine mutation and a 37 polyQ repeat

Depositor

Hilal Lashuel

Publication

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 fragment (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

A2C; 23 polyQ repeat

Available since

Oct. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pTWIN1-His6-Ssp-Htt2-90(A2C)-49Q

Plasmid

#114627

Purpose

Bacterial expression of Htt fragment containing amino acids 2-90, with A2C cysteine mutation and a 49 polyQ repeat

Depositor

Hilal Lashuel

Publication

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 fragment (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

A2C; 49 polyQ repeat

Available since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pTWIN1-His6-Ssp-Httex1-43Q-A2C

Plasmid

#114622

Purpose

Bacterial expression of Htt fragment containing exon 1, with A2C cysteine mutation and a 43 polyQ repeat

Depositor

Hilal Lashuel

Publication

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

A2C; 43 polyQ repeat

Available since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pTWIN1-His6-Ssp-Htt2-90-49Q-P90C

Plasmid

#114631

Purpose

Bacterial expression of Htt fragment containing amino acids 2-90, with P90C cysteine mutation and a 49 polyQ repeat

Depositor

Hilal Lashuel

Publication

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 fragment (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

P90C; 49 polyQ repeat

Available since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pTWIN1-His6-Ssp-Htt2-90-15Q-P90C

Plasmid

#114611

Purpose

Bacterial expression of Htt fragment containing amino acids 2-90, with P90C cysteine mutation and a 15 polyQ repeat

Depositor

Hilal Lashuel

Publication

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 fragment (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

P90C; 23 polyQ repeat

Available since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pMAPLe4 PCMTD1

Plasmid

#246503

Purpose

Recombinant protein expression of PCMTD1

Depositor

Steven Clarke

Publication

Warmack et al Biochemistry. 2022 May 17;61(10):879-894

Insert

Protein-L-isoaspartate O-methyltransferase domain-containing protein 1 (PCMTD1 Human)

Tags

His-tag

Expression

Bacterial

Mutation

Residue 312 is Isoleucine in this plasmid but occ…

Available since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pET19pps-EndoIV(Thermus Thermophilus)

Plasmid

#236048

Purpose

pET19pps-APE1(Thermus Thermophilus)

Depositor

Luis Gabriel Brieba

Publication

Cordoba-Andrade et al PeerJ. 2025 Aug 13;13:e19758. doi: 10.77

Insert

Endo IV from Thermus thermophilus

Tags

His tag and Histidine tag

Expression

Bacterial

Promoter

T7 RNA polymerase

Available since

Aug. 1, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5-FRT/TO-N-mEGFP-PARP6

Plasmid

#178005

Purpose

Expresses N-terminally mEGFP-tagged PARP6

Depositor

Karla Feijs

Publication

Weixler et al Life Sci Alliance. 2022 Nov 11;6(1):e202

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PARP6 (PARP6 Human)

Expression

Mammalian

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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A3B-Cas9n-UGI-NLS

Plasmid

#198889

Purpose

Express full-length A3B BE3-based base editor

Depositor

Reuben Harris

Publication

Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-like 3B (APOBEC3B Human)

Tags

NLS

Expression

Mammalian

Promoter

CMV

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP ratAPOBEC1 L173A/G227A

Plasmid

#167169

Purpose

Expresses rat APOBEC1 L173A/G227A in mammalian cells. Please note that this does not express GFP.

Depositor

Silvestro Conticello

Publication

Chieca et al bioRxiv 2021

Insert

APOBEC1 (Apobec1 Rat)

Expression

Mammalian

Mutation

changed Leucine 173 to Alanine; changed Glycine 2…

Promoter

CMV promoter

Available since

May 10, 2022

Availability

Academic Institutions and Nonprofits only

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pTWIN1-His6-Ssp-Httex1-23Q-A2C-A60C

Plasmid

#114613

Purpose

Bacterial expression of Htt fragment containing exon 1, with A2C & A60C cysteine mutations and a 23 polyQ repeat

Depositor

Hilal Lashuel

Publication

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

A2C, A60C; 23 polyQ repeat

Available since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pTWIN1-His6-Ssp-Httex1-43Q-A2C-A60C

Plasmid

#114623

Purpose

Bacterial expression of Htt fragment containing exon 1, with A2C & A60C cysteine mutations and a 43 polyQ repeat

Depositor

Hilal Lashuel

Publication

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

A2C, A60C; 43 polyQ repeat

Available since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pCfB6371

Plasmid

#106153

Purpose

EasyCloneYALI system-based yeast integrative vector for markerfree integration via CRISPR/Cas9 to be used in combination with pCfB6630, integration into Yarrowia lipolytica chromosomal location IntC_3, USER site for cloning, amp resistance

Depositor

Irina Borodina

Publication

Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

Type

Empty backbone

Expression

Yeast

Available since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pFW5000

Plasmid

#224214

Purpose

E. coli-Bacteroides shuttle plasmid; pB8-51 (Bacteroides replication origin), p15A (E. coli replication origin), tetQ

Depositor

Ophelia Venturelli

Publication

Feng et al Cell Host Microbe. 2022 Feb 9;30(2):200-

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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C079

Plasmid

#174348

Purpose

Template for making mRNA for overexpressing eGFP in frog oocytes

Depositor

David Bartel

Publication

Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

Insert

eGFP

Use

In vitro transcription template

Available since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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pLyGo-Ec-6

Plasmid

#163132

Purpose

pLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding PelB signal peptide and the LyGo cassette (SapI-ccdB-SapI)

Depositor

Morten Norholm

Publication

Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7

Available since

July 30, 2021

Availability

Academic Institutions and Nonprofits only

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pJZ002

Plasmid

#120231

Purpose

CRISPR-cas9 targeting in E. coli with ampicillin resistance

Depositor

Bradley Moore

Publication

Bauman et al Cell Chem Biol. 2019 Feb 15. pii: S2451-

Type

Empty backbone

Use

CRISPR

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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AMBERv2 (pICSL80189)

Plasmid

#252516

Purpose

Level 0 plasmid containing AMBERv2 CDS (pICSL80189)

Depositor

Sophien Kamoun

Publication

Desnoyer et al bioRxiv 2026.01.14.699277

Insert

AMBERv2

Expression

Plant

Available since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

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pRK5R MCS+

Plasmid

#211354

Purpose

empty vector control

Depositor

Raghu Chivukula

Publication

Yang et al Science. 2025 Jul 17;389(6757):eado2403.

Type

Empty backbone

Expression

Mammalian

Promoter

CMV

Available since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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