We narrowed to 6,081 results for: nls

1. AmCyan-P2A-mCherry

   Plasmid

   #45350

   Depositor

   Ilpo Huhtaniemi

   Article

   Potorac et al J Endocrinol. 2016 Sep 21. pii: JOE-16-0

   Insert

   AmCyan
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   SV40 NLS

   Promoter

   CMV

   Available since

   Nov. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLX208-cytoFLARE1.0-TF

   Plasmid

   #234513

   Purpose

   Transcriptional reporter for detecting calcium increase in HEK293T (transcription factor)

   Depositor

   Wenjing Wang

   Article

   Zhou et al Cell Rep Methods. 2025 Apr 21;5(4):10101

   Insert

   ERT2-MK2-hLOV-TEVcs-NLS-GAL4-FLAG
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 3, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. pCMV-PE2-VRQR

   Plasmid

   #159983

   Purpose

   Prime editing in mammalian cells

   Depositor

   Yongsub Kim

   Article

   Kweon et al Mol Ther. 2021 Feb 23. pii: S1525-0016(2

   Insert

   PE2-VRQR
   

   Use

   Tags

   BpSV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCMV-PE2-SpRY

   Plasmid

   #159979

   Purpose

   Prime editing in mammalian cells

   Depositor

   Yongsub Kim

   Article

   Kweon et al Mol Ther. 2021 Feb 23. pii: S1525-0016(2

   Insert

   PE2-SpRY
   

   Use

   Tags

   BpSV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pJL-SaKKH-BE3

   Plasmid

   #85170

   Purpose

   Expresses SaKKH-BE3 in mammalian cells [Edit]

   Depositor

   David Liu

   Article

   Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

   Insert

   SaKKH-BE3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCMV-PE2-NG

   Plasmid

   #159977

   Purpose

   Prime editing in mammalian cells

   Depositor

   Yongsub Kim

   Article

   Kweon et al Mol Ther. 2021 Feb 23. pii: S1525-0016(2

   Insert

   PE2-NG
   

   Use

   Tags

   BpSV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pPIK16045_pX330_Flag-SuperFi-Cas9

   Plasmid

   #184370

   Purpose

   Mammalian expression of SuperFi-Cas9 nuclease

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:

   Insert

   SuperFi-Cas9
   

   Use

   CRISPR

   Tags

   3X FLAG and NLS

   Expression

   Mammalian

   Mutation

   Y1010D, Y1013D, Y1016D, V1018D, R1019D, Q1027D, K…

   Promoter

   CBh

   Available since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pYPQ152

   Plasmid

   #69303

   Purpose

   Gateway entry vector with pco-dCas9-VP64

   Depositor

   Yiping Qi

   Article

   Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

   Insert

   pco-dCas9-VP64 (Plant codon-optimized)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   VP64 activator fusion; NLS fusion

   Promoter

   Available since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAT253

   Plasmid

   #40943

   Depositor

   Angela Taddei

   Article

   Dubarry et al Genes Dev. 2011 Jul 1;25(13):1365-70.

   Insert

   GFP-LacI**
   

   Use

   Tags

   GFP(S65T, V163A) and SV40 NLS

   Expression

   Yeast

   Mutation

   LacI**/LacI2G(4 mutations) (see comment below); l…

   Promoter

   His3 promoter fragment

   Available since

   Feb. 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3-nuclear-EKAR4

    Plasmid

    #174439

    Purpose

    Nuclear-targeted EKAR4.

    Depositor

    Jin Zhang

    Article

    Keyes et al eLife. 2020 May 26;9. pii: 57410. doi: 1

    Insert

    nuclear-EKAR4
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBK-VQR-BE3

    Plasmid

    #85171

    Purpose

    Expresses VQR-BE3 in mammalian cells

    Depositor

    David Liu

    Article

    Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

    Insert

    VQR-BE3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSL4393 (pClpX_Eco)

    Plasmid

    #200907

    Purpose

    Expresses human codon-optimized E. coli ClpX from a CMV promoter.

    Depositor

    Samuel H. Sternberg

    Article

    Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

    Insert

    EcoClpX
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Bxb1-GA_AIO-TetOn_mScarlet_Divergent

    Plasmid

    #229791

    Purpose

    Bxb1-GA donor plasmid with divergent syntax for all-in-one doxycycline-inducible mScarlet expression

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    TRE-mScarlet; rtTA-T2A-mTagBFP2
    

    Use

    Synthetic Biology

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    TRE and CAG

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Ubiq-Cas9.874W

    Plasmid

    #112686

    Purpose

    Express hSpCas9 under Ubiquitin-63E promoter

    Depositor

    Omar Akbari

    Article

    Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

    Insert

    hSpCas9
    

    Use

    Tags

    3x FLAG, EGFP, and NLS

    Expression

    Insect

    Mutation

    Humanized Cas9 bacterial sequence

    Promoter

    Ubiquitin-63E promoter

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. 3xFLAG-dCas9/pMSCVhyg

    Plasmid

    #134323

    Purpose

    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

    Depositor

    Hodaka Fujii

    Article

    Yuno et al Biol Methods Protoc. 2021 Jul 9;6(1):bpa

    Insert

    3xFLAG-dCas9
    

    Use

    CRISPR and Retroviral

    Tags

    3xFLAG tag and NLS

    Expression

    Mammalian

    Mutation

    human codon-optimized, D10A + H840A

    Promoter

    LTR

    Available since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pYPQ167

    Plasmid

    #69309

    Purpose

    Gateway entry vector with Cas9p

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Insert

    Cas9p (Plant codon-optimized; high GC content at 5 prime region)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    NLS fusion

    Promoter

    Available since

    Sept. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCMV-PE2-VQR

    Plasmid

    #159982

    Purpose

    Prime editing in mammalian cells

    Depositor

    Yongsub Kim

    Article

    Kweon et al Mol Ther. 2021 Feb 23. pii: S1525-0016(2

    Insert

    PE2-VQR
    

    Use

    Tags

    BpSV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSQT834

    Plasmid

    #53371

    Purpose

    Csy4 and Cas9 nuclease expression plasmid

    Depositor

    Keith Joung

    Article

    Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

    Insert

    Csy4-T2A-Cas9-NLS
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. 3xFLAG-dCas9/pMXs-puro

    Plasmid

    #51240

    Purpose

    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

    Depositor

    Hodaka Fujii

    Article

    Fujita et al PLoS One. 2014 Jul 22;9(7):e103084. doi:

    Insert

    3xFLAG-dCas9
    

    Use

    CRISPR and Retroviral

    Tags

    3xFLAG tag and NLS (nuclear localization signal)

    Expression

    Mammalian

    Mutation

    human codon-optimized, D10A + H840A

    Promoter

    LTR

    Available since

    Aug. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pREDIT_MCP-RecT

    Plasmid

    #164803

    Purpose

    mammalian-optimized expression of SSAP RecT fused to MCP (for MS2 aptamer), pLenti-EF1A-MCP-EXTEN-RecT-NLS

    Depositor

    Le Cong

    Article

    Wang et al Nucleic Acids Res. 2021 Apr 6;49(6):e36.

    Insert

    RecT
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge