Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 18,211 results for: puro

Showing: 3801 - 3820 of 18211 results

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pHAGE-IRES-puro-NLS-dLwaCas13a-EGFP-NLS-3xFlag

Plasmid

#132407

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dLwaCas13b

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

R474A; H479A; R1046A; R1051A

Available Since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pLY092b-lenti-EFS-BCMA-BBz-T2APRODH2-T2APuro-WPRE

Plasmid

#192193

Purpose

Lenti-BCMA-CAR-PRODH2

Depositor

Sidi Chen

Article

Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

Insert

Lenti-BCMA-CAR-PRODH2 (TNFRSF17 Synthetic)

Use

Lentiviral; Mammalian expression

Mutation

NA

Available Since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1alpha-SARS-CoV-2-orf3a-2xStrep-IRES-Puro

Plasmid

#141383

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-orf3a (ORF3a SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available Since

April 8, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pLVX-EF1alpha-SARS-CoV-2-nsp2-2xStrep-IRES-Puro

Plasmid

#141368

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-nsp2 (ORF1ab SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available Since

April 8, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pLVX-EF1alpha-SARS-CoV-2-orf8-2xStrep-IRES-Puro

Plasmid

#141390

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-orf8 (ORF8 SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available Since

April 8, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pLVX-EF1alpha-SARS-CoV-2-orf7a-2xStrep-IRES-Puro

Plasmid

#141388

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-orf7a (ORF7a SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available Since

April 9, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pSLQ8454 pHR-hU6-LbCpf1 crRNA BB-EF1a-Puro-WPRE

Plasmid

#140087

Purpose

Backbone for cloning U6 driven LbCpf1 (Cas12a) crRNA, with EF1a driven puromycin resistance

Depositor

Stanley Qi

Article

Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

Insert

LbCpf1 (LbCas12a) crRNA backbone

Use

Lentiviral

Tags

EF1a-Puro

Expression

Mammalian

Promoter

U6

Available Since

May 7, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-HyperdLbCas12a-StayGold-NLS-3xFlag

Plasmid

#236368

Purpose

Overexpression of HyperdLbCas12a in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

Insert

HyperdLbCas12a

Use

CRISPR and Lentiviral

Mutation

D156R, D235R, E292R, D350R

Available Since

July 15, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dPspCas13b-EGFP-NLS-3xFlag

Plasmid

#132397

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dPspCas13b

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

H133A; H1058A

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-protein1-IRES-protein2-IRESatt-EGFP-2A-PuroR-SMAR-g5

Plasmid

#208377

Purpose

Episomal plasmid for expression of 2 proteins with puromycin selection and EGFP monitoring

Depositor

Daniel Hawiger

Article

Fallahee et al Antibodies (Basel). 2024 Mar 11;13(1):18

Type

Empty backbone

Expression

Mammalian

Promoter

human CMV

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dRfxCas13d-EGFP-NLS-3xFlag

Plasmid

#132411

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dRfxCas13b

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

R239A; H244A; R858A; H863A

Available Since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only

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tre-stblHIF: pLenti-TRE-stblHIF-PGK-rtTAA-2A-Puro

Plasmid

#177203

Purpose

encoding stblHIF under inducible promoter

Depositor

Markus Covert

Article

Kudo et al bioRxiv (2022)

Insert

stblHIF

Use

Lentiviral

Expression

Mammalian

Mutation

P402A/P577G/N813A

Promoter

TRE

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pLXV-EF1alpha-2xStrep-SARS-CoV-2-nsp14-IRES-Puro

Plasmid

#141380

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-nsp14 (ORF1ab SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available Since

April 9, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pLVX-EF1alpha-SARS-CoV-2-nsp10-2xStrep-IRES-Puro

Plasmid

#141376

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-nsp10 (ORF1ab SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available Since

April 9, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pLVX-TREtight-Ngn2:2A:Ascl1-PGK-Puro (XTP-N2A)

Plasmid

#84777

Purpose

Second generation lenti expressing Mash1 and Neurogenin for iN transdifferentation to neurons

Depositor

Fred Gage

Article

Mertens et al Cell Stem Cell. 2015 Oct 6. pii: S1934-5

Insert

Ngn2 and Mash1 with a T2A (NEUROG2, ASCL1 Human)

Use

Lentiviral

Promoter

pTIGHT

Available Since

Nov. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1alpha-SARS-CoV-2-nsp6-2xStrep-IRES-Puro

Plasmid

#141372

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-nsp6 (ORF1ab SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available Since

Sept. 17, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pLVX-EF1alpha-SARS-CoV-2-orf6-2xStrep-IRES-Puro

Plasmid

#141387

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-orf6 (ORF6 SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available Since

April 9, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pLVX-EF1alpha-SARS-CoV-2-orf9b-2xStrep-IRES-Puro

Plasmid

#141392

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-orf9b

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available Since

April 8, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pLVX-EF1alpha-SARS-CoV-2-nsp4-2xStrep-IRES-Puro

Plasmid

#141369

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-nsp4 (ORF1ab SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available Since

April 8, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pLVX-EF1alpha-SARS-CoV-2-nsp9-2xStrep-IRES-Puro

Plasmid

#141375

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-nsp9 (ORF1ab SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available Since

April 8, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge

Showing: 3801 - 3820 of 18211 results

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