We narrowed to 14,099 results for: CAN
-
Plasmid#218629PurposeThis plasmid contains human codon optimized sequence of mitoEcSTH which is a soluble transhydrogenase from E. coli that can be used to increase NADH/NAD+ ratio in mammalian cells.DepositorInsertmitoEcSTH
UseLentiviral and Synthetic BiologyTagsFLAG and MTSExpressionMammalianAvailable SinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLKO-Cre stuffer v4
Plasmid#158032Purposelenti-viral construct with Cre recombinase and U6 driven sgRNA casette with modified tracrRNA (20bp sgRNA can be cloned by digesting the stuffer 1.8kb with BsmBI). NO Cas9DepositorTypeEmpty backboneExpressionMammalianAvailable SinceSept. 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pX261-U6-DR-hEmx1-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro
Plasmid#42337PurposeDual expression plasmid of human codon-optimized SpCas9 and a gRNA to the human Emx1 locus, can be used to test SpCas9 cleavage in cell lines of choice.DepositorArticleInserthumanized S. pyogenes Cas9
UseCRISPRTags3xFLAGExpressionMammalianAvailable SinceFeb. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-130b-3p
Plasmid#103217PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-130b-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-130b-3p target (MIR130B Human)
UseSynthetic BiologyExpressionMammalianPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-218-5p
Plasmid#103357PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-218-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-218-5p target
UseSynthetic BiologyExpressionMammalianPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-EGFP-NLS-P2A-mCherry-PTS1
Plasmid#87803PurposeMammalian expression vector template for co-expression of EGFP-tagged and mCherry-tagged proteins using P2A. NLS and PTS1 can be replaced by proteins of interest.DepositorInsertsNLS
PTS1
TagsEGFP and mCherryExpressionMammalianPromoterCMV promoter, tetracycline operatorAvailable SinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pmScarlet-i_H2A_C1
Plasmid#85053PurposeIn vivo visualization of the nucleus (can be used for colocalization studies)DepositorAvailable SinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pIDTSmart-MbPylRS
Plasmid#217361PurposeExpresses Methanosarcina barkeri aminoacyl-tRNA synthetases; can be packaged into bacmidDepositorInsertM. barkeri pyrrolysyl-tRNA synthetase
ExpressionMammalianPromoterCMVAvailable SinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
mCherry-p2A-CreERT2-FRT-kan-FRT
Plasmid#108454PurposeThis plasmid can be used to recombine the mCherry-p2a-CreERT2-pA cassette (or only CreERT2-pA) into a BAC. Also to produce mCherry-p2A-CreERT2 mRNA using SP6.DepositorInsertmCherry-p2a-CreERT2
UseCre/LoxAvailable SinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-30d-5p
Plasmid#103417PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-30d-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-30d-5p target (MIR30D Human)
UseSynthetic BiologyExpressionMammalianPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only