We narrowed to 38,498 results for: LAT;

1. pcDNA-Cx43-YA/VD-GFP

   Plasmid

   #60703

   Purpose

   mammalian expression of rat Cx43 YA/VD mutant fused to GFP

   Depositor

   Parmender Mehta

   Article

   Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

   Insert

   Cx43 (Gja1 Rat)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   YA/VD (Y286A/V289D)

   Promoter

   CMV

   Available since

   July 6, 2015

   Availability

   Academic Institutions and Nonprofits only

2. Pyr pUAST-HA

   Plasmid

   #69768

   Purpose

   Pyramus (FGF8-like-2) Ligand in P element-based pUAST vector for Gal4-regulated expression in Drosophila.

   Depositor

   Arno Muller

   Article

   Klingseisen et al Development. 2009 Jul;136(14):2393-402.

   Insert

   Pyramus (FGF8like-2) (pyr Fly)
   

   Use

   P element-based puast vector for gal4-regulated e…

   Tags

   HA-Tag

   Expression

   Insect

   Mutation

   241T amino acid insertion compared to reference s…

   Promoter

   hsp70 promoter

   Available since

   Nov. 24, 2015

   Availability

   Academic Institutions and Nonprofits only

3. INSR-bio-His

   Plasmid

   #53341

   Purpose

   Expresses full-length Insulin receptor precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   INSR (INSR Human)
   

   Use

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. FCGR2a-bio-His

   Plasmid

   #51754

   Purpose

   Expresses full-length Low affinity immunoglobulin gamma Fc region receptor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   FCGR2a (FCGR2A Human)
   

   Use

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pJWK_VD_01

   Plasmid

   #158102

   Purpose

   5'UTR stem-loop luciferase reporter: 0kcal/mol

   Depositor

   Ted Dawson

   Article

   Kim et al Cell Stem Cell. 2020 Oct 1;27(4):633-645

   Insert

   5'UTR synthetic stem-loop 0kcal/mol
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. His6tev CBP BCHZDeltaAL

   Plasmid

   #99551

   Purpose

   Bacterial expression of His tagged mouse CBP HAT Brd-ZZ(1079-1751) 1557-1617 replaced by SGGSG

   Depositor

   Peter Wright

   Article

   Park et al Proc Natl Acad Sci U S A. 2017 Jun 19. p

   Insert

   mouse CBP BRD-ZZ domains (1079-1751) DeltaAL (Crebbp Mouse)
   

   Use

   Tags

   His6tev

   Expression

   Bacterial

   Mutation

   replaced 1557-1618 with SGGSG

   Promoter

   T7

   Available since

   June 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. SMN1-1

   Plasmid

   #156022

   Purpose

   For use in RBP tethering screen

   Depositor

   Eugene Yeo

   Article

   Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

   Insert

   SMN1 (SMN1 Human)
   

   Use

   Tags

   V5-MCP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   EF-1a

   Available since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDN-G1TGt

   Plasmid

   #44508

   Depositor

   Gabor Balazsi

   Article

   Nevozhay et al Proc Natl Acad Sci U S A. 2009 Mar 31;10

   Inserts

   PGal1-D12 promoter (GAL1 Budding Yeast, Synthetic)
   tetR::yEGFP
   

   Use

   Synthetic Biology; Expression regulator/reporter

   Tags

   Expression

   Yeast

   Mutation

   Two tandem tet operators (2XtetO2) downstream of …

   Promoter

   PGal1-D12 promoter

   Available since

   April 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. LXSN-Cx43-S279/282A

   Plasmid

   #60713

   Purpose

   retroviral expression of rat Cx43 S279/282A mutant

   Depositor

   Parmender Mehta

   Article

   Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

   Insert

   Cx43 (Gja1 Rat)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   S279/282A

   Promoter

   LTR

   Available since

   July 6, 2015

   Availability

   Academic Institutions and Nonprofits only

10. LXSN-Cx43-S279/282D

    Plasmid

    #60715

    Purpose

    retroviral expression of rat Cx43 S279/282D mutant

    Depositor

    Parmender Mehta

    Article

    Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

    Insert

    Cx43 (Gja1 Rat)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    S279/282D

    Promoter

    LTR

    Available since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pT7-7 asyn S87E

    Plasmid

    #36059

    Depositor

    Hilal Lashuel

    Article

    Paleologou et al J Neurosci. 2010 Mar 3;30(9):3184-98.

    Insert

    alpha-synuclein S87E (SNCA Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    S87E

    Promoter

    T7

    Available since

    July 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMT-zic2a-cytoBirA-2A-mCherry_Ras

    Plasmid

    #80064

    Purpose

    Zic2a promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
    

    Use

    Zebrafish biotagging

    Tags

    3x HA

    Expression

    Bacterial

    Mutation

    Promoter

    Zic2a enhancer driving c-fos promoter

    Available since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTER GAMMA-TUBULIN shRNA Human

    Plasmid

    #87955

    Purpose

    reduced the expression of gamma-tubulin in human cells

    Depositor

    Ana Carrera, Maria Alvarado-Kristensson

    Article

    Alvarado-Kristensson et al Nat Cell Biol. 2009 Sep;11(9):1081-92. d

    Insert

    sh gamma-tubulin (TUBG1 Human)
    

    Use

    Tags

    No-tag

    Expression

    Mammalian

    Mutation

    The protein is a sh-gamma-tubulin resistant gene.…

    Promoter

    Available since

    Oct. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV-EF1a-double floxed-ClopHensorN-WPRE-HGHpA

    Plasmid

    #193728

    Purpose

    Cre-activated AAV expression of ClopHensorN for measuring intracellular chloride and pH in genetically-defined cell types

    Depositor

    Colin Akerman

    Article

    Calin et al iScience. 2023 Mar 9;26(4):106363. doi:

    Insert

    ClopHensorN
    

    Use

    AAV

    Tags

    Strep-II (N terminal on insert)

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pS6-C-hα2M-H6

    Plasmid

    #128497

    Purpose

    Expresses human C-α2M (from F789 to S1468) in mammalian cells. With C-t H6x tag.

    Depositor

    F Xavier Gomis-Ruth

    Article

    Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

    Insert

    A2M (F789-S1468) (A2M Human)
    

    Use

    Tags

    His tag (6x)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. LXSN-Cx43-mAPPLE

    Plasmid

    #60696

    Purpose

    retroviral expression of rat Cx43 fused to mAPPLE

    Depositor

    Parmender Mehta

    Article

    Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

    Insert

    Cx43 (Gja1 Rat)
    

    Use

    Retroviral

    Tags

    mAPPLE

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pMT-ubb-NLS-BirA-2a-mCherry

    Plasmid

    #79886

    Purpose

    Ubiquitin promoter driving HA-tagged biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein; flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
    

    Use

    Tags

    3x HA

    Expression

    Bacterial

    Mutation

    Promoter

    Ubiquitin

    Available since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLL5.0 hCortKD-Flcortmut3YF EGFP

    Plasmid

    #187263

    Purpose

    Lentiviral expression of shRNA targeting Cortactin + reconstitution of Cortactin3YF mutant

    Depositor

    Alpha Yap

    Article

    Liang et al Nat Commun. 2017 Oct 5;8(1):790. doi: 10

    Insert

    Cortactin (CTTN Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mutation

    3YF (Y412, Y470, Y486)

    Promoter

    U6, 5'LTR

    Available since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMT-MCS-NLS-BirA-2A-mCherry_Ras

    Plasmid

    #80059

    Purpose

    MCS for cloning promoter to drive HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
    

    Use

    Zebrafish biotagging

    Tags

    3x HA

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. DMGDH

    Plasmid

    #155549

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    DMGDH (DMGDH Human)
    

    Use

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge