We narrowed to 24,606 results for: Nes

1. p2x35S (GB0222)

   Plasmid

   #68173

   Purpose

   Provides the CaMV dual 35S Promoter as a level 0 GoldenBraid part

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   2x35S promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available Since

   March 16, 2016

   Availability

   Academic Institutions and Nonprofits only

2. p6xUAS (GB0179)

   Plasmid

   #68182

   Purpose

   Provides 6xUAS (upstream activating sequence) as a level 0 GoldenBraid part

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   6xUAS
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available Since

   March 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDGB3_omega1R

   Plasmid

   #68240

   Purpose

   GoldenBraid level 1 Omega plant expression vector

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   empty vector
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Oct. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pT2A11 (GB0143)

   Plasmid

   #68189

   Purpose

   Provides the S. Lycopersicum 2A11 terminator as a level 0 GoldenBraid part

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   Sl2A11 terminator
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available Since

   Oct. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pTE8 (GB0144)

   Plasmid

   #68190

   Purpose

   Provides the S. Lycopersicum E8 terminator as a level 0 GoldenBraid part

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   SlE8 terminator
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available Since

   Oct. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLexAOp (GB0118)

   Plasmid

   #68181

   Purpose

   Provides the 4xLexA Operator as a level 0 GoldenBraid part

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   4xOpLexA
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available Since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDGB3_omega2R

   Plasmid

   #68241

   Purpose

   GoldenBraid level 1 Omega plant expression vector

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   empty vector
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Oct. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Nano-lantern(Ca2+)_63/pRSETB

   Plasmid

   #51980

   Purpose

   bacterial expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=63nM

   Depositor

   Takeharu Nagai

   Article

   Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

   Insert

   Nano-lantern_CaM_E104Q-4GS
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   S257G in Rluc8

   Promoter

   Available Since

   March 25, 2014

   Availability

   Academic Institutions and Nonprofits only

9. Khc73(1-387)-GFP

   Plasmid

   #31747

   Depositor

   Ron Vale

   Article

   Huckaba et al J Biol Chem. 2011 Mar 4;286(9):7457-67.

   Insert

   Khc73 (Khc-73 Fly)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Contains only aa 1-387

   Promoter

   Metallothionein

   Available Since

   Nov. 8, 2011

   Availability

   Academic Institutions and Nonprofits only

10. Khc73(1-432)-GFP

    Plasmid

    #31748

    Depositor

    Ron Vale

    Article

    Huckaba et al J Biol Chem. 2011 Mar 4;286(9):7457-67.

    Insert

    Khc73 (Khc-73 Fly)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Contains only aa 1-432

    Promoter

    Metallothionein

    Available Since

    Nov. 8, 2011

    Availability

    Academic Institutions and Nonprofits only

11. pEN_TTmiRC2_3xflag_Luciferase

    Plasmid

    #136519

    Purpose

    Used as a donor vector containing N-term 3xFLAG to clone into pSLIK

    Depositor

    Kevin Janes

    Article

    Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

    Insert

    Luciferase
    

    Use

    Entry vector for gateway cloning

    Tags

    3x FLAG

    Expression

    Mutation

    Promoter

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJK412

    Plasmid

    #155371

    Purpose

    dCas9 oscillator v1 (dCRv1) + [dCas9 + PA4-mVenus]

    Depositor

    Pamela Silver

    Article

    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

    Inserts

    dCas9 (bacteria)
    PA4-mVenus
    dCas9 sgRNA oscillator v1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    D10A H840A (catalytically inactive)

    Promoter

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJK430

    Plasmid

    #155377

    Purpose

    PphlF_A2NT in dCRv1, 2x cascade + [dCas9 + PA4-mVenus]

    Depositor

    Pamela Silver

    Article

    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

    Inserts

    dCas9 (bacteria)
    PA4-mVenus
    PphlF_A2NT in dCas9 sgRNA oscillator v1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    D10A H840A (catalytically inactive)

    Promoter

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. PB-TO-hNGN2

    Plasmid

    #172115

    Purpose

    Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression

    Depositor

    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

    Article

    Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1

    Inserts

    hNGN2 (NEUROG2 Human)
    puroR-T2A-mycNLS-mTagBFP2
    

    Use

    Tags

    T2A-mycNLS-mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a and TRE3G

    Available Since

    April 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. PB-PG13-mCherry-EF1a-PuroR-EGFP

    Plasmid

    #195292

    Purpose

    piggyBac transposon vector with mCherry reporter containing 13 copies of the p53-binding consensus sequence (constitutive puromycin resistance and EGFP)

    Depositor

    Hideyuki Oguro

    Article

    Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1

    Inserts

    mCherry
    PuroR-P2A-EGFP
    

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. RalGDS-YPet

    Plasmid

    #134929

    Purpose

    Fluorescently tagged affinity reagent

    Depositor

    Klaus Hahn

    Article

    O'Shaughnessy et al J Cell Biol. 2019 Sep 2;218(9):3153-3160

    Insert

    RaIGDS RBD, YPet
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGEX-4T-1-3xFLAG-HSP27

    Plasmid

    #129229

    Purpose

    Encodes FLAG tagged human HSP27 for purification by GST affinity

    Depositor

    Kevin Janes

    Article

    Smolko et al Sci Rep. 2019 Dec 19;9(1):19409. doi: 10

    Insert

    HSP27 (HSPB1 Human)
    

    Use

    Tags

    FLAG and GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pETM6-RgTALsyn

    Plasmid

    #100947

    Purpose

    Expression of Tyrosine Ammonia Lyase from inducible T7 promoter

    Depositor

    Mattheos Koffas

    Article

    Jones et al MBio. 2017 Jun 6;8(3). pii: e00621-17. d

    Insert

    RgTALsyn
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Silent mutation to remove internal NdeI site on T…

    Promoter

    T7

    Available Since

    Sept. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pYES260-sc.eEF2

    Plasmid

    #85119

    Purpose

    yeast expression of EEF2

    Depositor

    Pal Falnes

    Article

    Davydova et al J Biol Chem. 2014 Oct 31;289(44):30499-5

    Insert

    EFT1/EFT2 (EFT2, EFT1 Budding Yeast)
    

    Use

    Tags

    6xHis

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available Since

    Nov. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEN_TT 3xFLAG-TWIST2

    Plasmid

    #197935

    Purpose

    Gateway entry vector for an inducible 3xFLAG-tagged TWIST2

    Depositor

    Kevin Janes

    Article

    Dutta et al Genome Res. 2023 Feb 21;33(3):314-31. do

    Insert

    TWIST2 (TWIST2 Human)
    

    Use

    Gateway entry vector

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    Available Since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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