We narrowed to 5,747 results for: actin
-
Plasmid#206082Purposeexpression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and a mutation in the P loop of Domain IV which reduces trafficking to the cell surfaceDepositorAvailable SinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only
-
Cav2.2 HA EIA (E314A) pCAGGS
Plasmid#206081Purposeexpression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and a mutation in the P loop of Domain I which reduces trafficking to the cell surfaceDepositorAvailable SinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
Cav2.2 HA EIVK (E1658K) pCAGGS
Plasmid#206096Purposeexpression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and an E1658K mutation in the P loop of Domain IV which abolishes trafficking to the cell surfaceDepositorAvailable SinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pORANGE_NFL linker-3xFLAG KI
Plasmid#182679PurposeExpression of spCas9, gRNA targeting the end of mouse Nefl gene and donor linker-3xFLAG. Can be used for C-terminal tagging of endogenous NFL with a linker-3xFLAG tag.DepositorInsertNefl-targeting gRNA and linker-3xFLAG donor sequence (Nefl Mouse)
ExpressionMammalianPromoterU6 and chicken beta-actin promoterAvailable SinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDS255
Plasmid#34970DepositorInsertMid2(SS/TM)-GFP-LOVpep
ExpressionYeastMutationT406A, T407AAvailable SinceMarch 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCAG CAPN7 V18D-OSF
Plasmid#193042PurposeMammalian expression plasmid encoding full-length CAPN7 with V18D mutation that diminishes binding to IST1. Contains C-terminal OSF tag (One-Strep-FLAG)DepositorInsertCAPN7 (CAPN7 Human)
TagsOSF (One-Strep-FLAG)ExpressionMammalianMutationV18DPromoterChicken Beta-actinAvailable SinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG CAPN7 L61D-OSF
Plasmid#193043PurposeMammalian expression plasmid encoding full-length CAPN7 with L61D mutation. Contains C-terminal OSF tag (One-Strep-FLAG)DepositorInsertCAPN7 (CAPN7 Human)
TagsOSF (One-Strep-FLAG)ExpressionMammalianMutationL61DPromoterChicken Beta-actinAvailable SinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG CAPN7 F98D-OSF
Plasmid#193044PurposeMammalian expression plasmid encoding full-length CAPN7 with F98D mutation that diminishes binding to IST1. Contains C-terminal OSF tag (One-Strep-FLAG)DepositorInsertCAPN7 (CAPN7 Human)
TagsOSF (One-Strep-FLAG)ExpressionMammalianMutationF98DPromoterChicken Beta-actinAvailable SinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
BPK880-pCAG-DmrC-NLS-3xFLAG-VP64
Plasmid#136912PurposeDmrC with VP64 fused to it's C-terminus; Mammalian expression vectorDepositorInsertpCAG-DmrC-NLS-3xFLAG-VP64
UseCRISPRExpressionMammalianPromoterChicken Beta ActinAvailable SinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pORANGE_NFL linker(A6TAG)-3xFLAG KI
Plasmid#182680PurposeExpression of spCas9, gRNA targeting the end of mouse Nefl gene and donor linker(A6TAG)-3xFLAG. Can be used for amber codon suppression and click chemistry labeling of endogenous NFL.DepositorInsertNefl-targeting gRNA and linker(A6TAG)-3xFLAG donor sequence (Nefl Mouse)
ExpressionMammalianPromoterU6 and chicken beta-actin promoterAvailable SinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
GFP-Rab19
Plasmid#31741DepositorAvailable SinceSept. 21, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCIBN-tagBFP-hTRF2
Plasmid#103803PurposeBLInCR 'Localizer' construct that marks the telomeres and is targeted by a PHR-tagged effector upon illumination with blue lightDepositorExpressionMammalianMutationhTRF2: deletion of amino acids 1-42 and 476 compa…PromoterCMVAvailable SinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCas9-beta
Plasmid#169240PurposeCas9 and beta expression plasmid for performing gene knockouts via oligo recombineeringDepositorInsertscas9
beta
UseCRISPR and Synthetic BiologyExpressionBacterialAvailable SinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
PHB1-mCherry
Plasmid#232784PurposeEncodes an mCherry-tagged Prohibitin1/PHB1DepositorAvailable SinceMarch 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLexM-HsPQLC1(Δ100-125/GGGS)-FLAG
Plasmid#250422PurposeExpression of C-terminally FLAG-Tagged HsPQLC1 (Δ100-125 - with these residues replaced by a GGGS Linker) in Mammalian CellsDepositorInsertPQLC1 (SLC66A2 Human)
TagsFLAGExpressionMammalianMutationΔ100-125 (Replaced with GGGS)PromoterChicken-beta-actin promoter/CMV EnhancerAvailable SinceFeb. 6, 2026AvailabilityAcademic Institutions and Nonprofits only -
pLexM-HsPQLC1(Δ100-125/3xGGGS)-FLAG
Plasmid#250424PurposeExpression of C-terminally FLAG-Tagged HsPQLC1 (Δ100-125 - with these residues replaced by a 3xGGGS Linker) in Mammalian CellsDepositorInsertPQLC1 (SLC66A2 Human)
TagsFLAGExpressionMammalianMutationΔ100-125 (Replaced with 3xGGGS)PromoterChicken-beta-actin promoter/CMV EnhancerAvailable SinceFeb. 6, 2026AvailabilityAcademic Institutions and Nonprofits only -
pX461-ABE8e-nSpCas9-Intergenic sgRNA
Plasmid#237463PurposeAll-in-one base editor expressing adenine base editor (ABE8e-nSpCas9) and gRNA control targeting Intergenic siteDepositorInsertIntergenic sgRNA
UseCRISPRExpressionMammalianPromoterchicken beta-actin promoter & U6Available SinceJan. 12, 2026AvailabilityAcademic Institutions and Nonprofits only -
pX461-evoCDA1-nSpCas9-Intergenic sgRNA
Plasmid#237464PurposeAll-in-one base editor expressing cytosine base editor (evoCDA1-nSpCas9) and sgRNA control targeting Intergenic siteDepositorInsertIntergenic sgRNA
UseCRISPRExpressionMammalianPromoterchicken beta-actin promoter & U6Available SinceJan. 12, 2026AvailabilityAcademic Institutions and Nonprofits only -
pX462-ABE8e-nSaCas9-Intergenic sgRNA
Plasmid#237465PurposeAll-in-one base editor expressing adenine base editor (ABE8e-nSaCas9) and sgRNA control targeting Intergenic siteDepositorInsertIntergenic sgRNA
UseCRISPRExpressionMammalianPromoterchicken beta-actin promoter & U6Available SinceJan. 12, 2026AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-PHOSPHO2-TEV-Twin-Strep
Plasmid#222016PurposeExpress human PHOSPHO2 protein (C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-fusion protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK)) in mammalian cellDepositorInsertPHOSPHO2 (PHOSPHO2 Human)
TagsTEV cleavable site and Twin-Strep-tagExpressionMammalianMutationdeleted stop codon (TAA)Promoterchicken β-actin promoterAvailable SinceJan. 8, 2025AvailabilityAcademic Institutions and Nonprofits only