We narrowed to 17,568 results for: bon;

1. pZDonor-Nm-Cas9-gRNA-hU6

   Plasmid

   #61366

   Purpose

   empty backbone for cloning N. meningiditis protospacers. Encodes constant region of N. meningiditis Cas9 synthetic gRNA

   Depositor

   Charles Gersbach

   Article

   Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Promoter

   human U6

   Available Since

   April 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPBC-LG6s

   Plasmid

   #62809

   Purpose

   pPBC-LG6s expresses PiggyBac inverted terminal repeat-flanked Lck-GCaMP6s under the control of the CAG promoter.

   Depositor

   Karen Wilcox

   Article

   Gee et al Front Mol Neurosci. 2015 Apr 15;8:10. do

   Insert

   ITR-CAG-Lck-GCaMP6s-ITR
   

   Expression

   Bacterial and Mammalian

   Promoter

   CAG

   Available Since

   May 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV-GG-hUBC-EBFP2

   Plasmid

   #212314

   Purpose

   Lentiviral backbone with EBFP2 reporter

   Depositor

   Andrew Wilson

   Article

   Werder et al Sci Adv. 2022 Jul 15;8(28):eabo6566. doi

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV-CAG-dDIO-MCS--4x6T

   Plasmid

   #196422

   Purpose

   Empty vector for astrocyte-selective Dre-dependent AAV expression; astrocyte selectivity generated with 4x6T miRNA targeting cassette

   Depositor

   Stanley Thomas Carmichael

   Article

   Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

   Type

   Empty backbone

   Use

   AAV; Dre/rox; astrocyte-selective

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Feb. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAV10.KH

   Plasmid

   #63208

   Purpose

   pCACS backbone (Amp), Hyg, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into YKL162C-A (NotI).

   Depositor

   Jef Boeke

   Article

   Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial and Yeast

   Available Since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSLQ7751 pHR Ef1α: mCherry-P2A-AcrIIA4

   Plasmid

   #125148

   Purpose

   Lentiviral vector for constitutive expression of AcrIIA4 with mCherry fluorescent reporter.

   Depositor

   Stanley Qi

   Article

   Nakamura et al Nat Commun. 2019 Jan 14;10(1):194. doi:

   Insert

   AcrIIA4-P2A-mCherry
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   May 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. LC101: pMVP/PB/Neo-DEST

   Plasmid

   #121875

   Purpose

   pMVP destination vector, empty PiggyBac transposon vector backbone w/ Neo selection cassette

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Piggybac transposon, pmvp gateway destination vec…

   Expression

   Mammalian

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pENTR-2B-Tbx5

   Plasmid

   #98619

   Purpose

   Gateway entry vector TBX5

   Depositor

   Richard Harvey

   Article

   Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

   Insert

   TBX5 (Tbx5 Mouse)
   

   Use

   Entry vector

   Available Since

   July 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPBC-LG6f

   Plasmid

   #62808

   Purpose

   pPBC-LG6f expresses PiggyBac inverted terminal repeat-flanked Lck-GCaMP6f under the control of the CAG promoter.

   Depositor

   Karen Wilcox

   Article

   Gee et al Front Mol Neurosci. 2015 Apr 15;8:10. do

   Insert

   ITR-CAG-Lck-GCaMP6f-ITR
   

   Expression

   Bacterial and Mammalian

   Promoter

   CAG

   Available Since

   May 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. EC71171

    Plasmid

    #154069

    Purpose

    Level 0 Golden Gate vector, CRE with U5 intron at 254bp, in pICH41308 backbone

    Depositor

    Cristobal Uauy

    Article

    Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

    Insert

    CRE_U5intron
    

    Use

    Synthetic Biology

    Mutation

    All BsaI, Esp3I and BPiI restriction sites were r…

    Available Since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPBC-G3-tdT

    Plasmid

    #62814

    Purpose

    pPBC-G3-tdT expresses PiggyBac inverted terminal repeat-flanked GCaMP3 and tdTomato under the control of the CAG promoter.

    Depositor

    Karen Wilcox

    Article

    Gee et al Front Mol Neurosci. 2015 Apr 15;8:10. do

    Insert

    ITR-CAG-GCaMP3-IRES-tdTomato-ITR
    

    Expression

    Bacterial and Mammalian

    Promoter

    CAG

    Available Since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAV-hSyn-FlicR1

    Plasmid

    #74143

    Purpose

    Express FlicR1 preferentially in neurons

    Depositor

    Robert Campbell

    Article

    Abdelfattah et al J Neurosci. 2016 Feb 24;36(8):2458-72. d

    Insert

    FlicR1
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    April 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCS2+/N-Halo

    Plasmid

    #184417

    Purpose

    vector backbone to clone insert downstream of Halo tag (Halo tag codon optimized for Xenopus), for mRNA synthesis

    Depositor

    Ann Miller

    Article

    Dudley et al STAR Protoc. 2022 Aug 18;3(3):101622. do

    Type

    Empty backbone

    Use

    For mrna synthesis

    Tags

    Halo-tag

    Mutation

    Codon optimized for expression in Xenopus laevis …

    Available Since

    Aug. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCR4-huNFATc1nuc

    Plasmid

    #23988

    Depositor

    Jerry Crabtree

    Article

    Winslow et al Dev Cell. 2006 Jun . 10(6):771-82.

    Insert

    NFATc1 (NFATC1 Human)
    

    Use

    Cloning vector

    Tags

    EE tag

    Mutation

    S172A S175A S176A S178A S179A S181A S184A S187A S…

    Available Since

    March 24, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLCHKO

    Plasmid

    #155048

    Purpose

    Lentiviral backbone for cloning and expressing U6 driven hybrid guide (hg)RNAs with BfuAI cloning sites and puromycin selection.

    Depositor

    Jason Moffat, Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. LPCX BMP-RIB Q203D

    Plasmid

    #12642

    Depositor

    Rik Derynck

    Article

    Skillington et al J Cell Biol. 2002 Oct 14. 159(1):135-46.

    Insert

    BMP-RIB (Bmpr1b Mouse)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Q203D, activating mutation

    Available Since

    Oct. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

17. RRL.sin.cPPT.SFFV/Flag-E2-crimson.IRES-puro.WPRE (CG568)

    Plasmid

    #139445

    Purpose

    Lentiviral vector to ectopically express Flag-tagged E2-crimson from SFFV promoter

    Depositor

    Caroline Goujon

    Article

    Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

    Insert

    FLAG-tag E2-crimson
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pENTR4 TNRC6A

    Plasmid

    #20021

    Depositor

    Thomas Tuschl

    Article

    Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

    Insert

    Trinucleotide repeat containing 6A (TNRC6A Human)
    

    Use

    Entry vector

    Available Since

    Jan. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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19. LentiGuide-Puro-GFPg1 (BB09)

    Plasmid

    #139461

    Purpose

    Lentiviral vector with gRNA targeting GFP; includes puromycin selectable marker

    Depositor

    Caroline Goujon

    Article

    Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

    Insert

    GFP-targeting sgRNA inserted; resistance gene: puroR
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF-1a / U6

    Available Since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGAN201

    Plasmid

    #129204

    Purpose

    yTRAP sensor of [RNQ+], detects aggregation of Rnq1

    Depositor

    Ahmad Khalil

    Article

    Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

    Insert

    RNQ1 (RNQ1 Synthetic, Budding Yeast)
    

    Use

    Synthetic Biology

    Expression

    Yeast

    Promoter

    SUP35

    Available Since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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