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pAc5.1B-lambdaN-HA-DmTral-LSm-R21EE23KS13AI15A-V5His6_C

Plasmid

#146072

Purpose

Insect Expression of DmTral-LSm-R21EE23KS13AI15A

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

Insert

DmTral-LSm-R21EE23KS13AI15A (tral Fly)

Expression

Insect

Mutation

one silent mutation A198C compared to the sequenc…

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmTral-DFDF-KFKF_C

Plasmid

#146038

Purpose

Insect Expression of DmTral-DFDF-KFKF

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

Insert

DmTral-DFDF-KFKF (tral Fly)

Expression

Insect

Mutation

three silent mutations A198C, A387G, A1437G and 4…

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmTral-D30KT35A_C

Plasmid

#146042

Purpose

Insect Expression of DmTral-D30KT35A

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

Insert

DmTral-D30KT35A (tral Fly)

Expression

Insect

Mutation

two silent mutations A198C, A387G and a mutation …

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmTral-del46-60_C

Plasmid

#146043

Purpose

Insect Expression of DmTral-del46-60

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

Insert

DmTral-del46-60 (tral Fly)

Expression

Insect

Mutation

two silent mutations A198C, A387G and a mutation …

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmTral-F44A_C

Plasmid

#146044

Purpose

Insect Expression of DmTral-F44A

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

Insert

DmTral-F44A (tral Fly)

Expression

Insect

Mutation

two silent mutations A198C, A387G and a mutation …

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmTral-K73AD74A_C

Plasmid

#146045

Purpose

Insect Expression of DmTral-K73AD74A

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

Insert

DmTral-K73AD74A (tral Fly)

Expression

Insect

Mutation

two silent mutations A198C, A387G and a mutation …

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pnCS_mEGFP-DSep2_Peanut-Strep

Plasmid

#174496

Purpose

bacterial co-expression of Drosophila Sep2 fused to monomeric EGFP and of Drosophila Peanut

Depositor

Manos Mavrakis

Article

Mavrakis et al Methods Cell Biol. 2016;136:199-220. doi

Inserts

Sep2 (Sep2 Fly)
peanut (pnut Fly)

Tags

Strep and mEGFP

Expression

Bacterial

Available Since

Sept. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pET-DEST42-dre4

Plasmid

#170440

Purpose

Express drosophila dre4 in E.coli expression system. Generated from pDONR-dre4 by Gateway system. Used for custom antibody purification.

Depositor

Joan Conaway

Article

Tettey et al Cell Rep. 2019 Jun 25;27(13):3770-3779.e

Insert

dre4 (dre4 Fly)

Tags

6xHis and V5

Expression

Bacterial

Mutation

Deleted amino acids 1-20

Promoter

T7

Available Since

July 22, 2021

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmTral-R42E_B

Plasmid

#145988

Purpose

Insect Expression of DmTral-R42E

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

Insert

DmTral-R42E (tral Fly)

Expression

Insect

Mutation

two silent mutations A198C, A387G and a mutation …

Available Since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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pDEST-Hemmar-DSCP-ftz-FLP(D)

Plasmid

#119894

Purpose

FLP(D) with a Drosophila Synthetic Core Promoter (DSCP) and ftz intron

Depositor

Rita Sousa-Nunes

Article

Chai et al Elife. 2018 Nov 27;7. pii: 38393. doi: 1

Inserts

FLP(D)
DSCP-ftz (ftz Fly)

Use

Drosophila expression

Mutation

a mutated form of FLP, which at position 5 contai…

Available Since

Feb. 25, 2019

Availability

Academic Institutions and Nonprofits only

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mei-S332-GFP 5.6kb genomic DNA in Casper4 T331D

Plasmid

#112986

Purpose

P element vector for transposon insertion of mei-S332-GFP 5.6kb genomic DNA (T331D) into Drosophila genome

Depositor

Terry Orr-Weaver

Article

Nogueira et al G3 (Bethesda). 2014 Jul 31;4(10):1849-58

Insert

mei-S332 (mei-S332 Fly)

Use

P insertion vector

Tags

GFP

Mutation

T331D

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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mei-S332-GFP 5.6kb genomic DNA in Casper4 T331A

Plasmid

#112988

Purpose

P element vector for transposon insertion of mei-S332-GFP 5.6kb genomic DNA (T331A) into Drosophila genome

Depositor

Terry Orr-Weaver

Article

Nogueira et al G3 (Bethesda). 2014 Jul 31;4(10):1849-58

Insert

mei-S332 (mei-S332 Fly)

Use

P insertion vector

Tags

GFP

Mutation

T331A

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pMAL c2x Tral N

Plasmid

#113009

Purpose

For bacterial expression of MBP fusion of N terminal region of Drosophila Tral

Depositor

Terry Orr-Weaver

Article

Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

Insert

N terminus of tral (tral Fly)

Expression

Bacterial

Mutation

amino acids 1-355

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pMAL c2x Tral FL-15A

Plasmid

#113011

Purpose

For bacterial expression of MBP fusion of full-length Drosophila Tral with phosphomutations (15A)

Depositor

Terry Orr-Weaver

Article

Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

Insert

full length tral (tral Fly)

Expression

Bacterial

Mutation

15 PNG phosphorylation sites mutated to A

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pMAL c2x Tral C

Plasmid

#113008

Purpose

For bacterial expression of MBP fusion of C terminal region of Drosophila Tral

Depositor

Terry Orr-Weaver

Article

Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

Insert

C terminus of tral (tral Fly)

Expression

Bacterial

Mutation

amino acids 356-652

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pMAL c2x Tral C 15A

Plasmid

#113007

Purpose

For bacterial expression of MBP fusion of C terminal region of Drosophila Tral phosphomutant (15A)

Depositor

Terry Orr-Weaver

Article

Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

Insert

C terminus of tral (tral Fly)

Expression

Bacterial

Mutation

PNG phosphorylation sites mutated to A

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pFOSGE_COF4D03

Plasmid

#70969

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Taf12 (Taf12 Fly)

Use

Gateway entry vector

Available Since

Dec. 16, 2015

Availability

Academic Institutions and Nonprofits only
pFOSGE_CUS1F03

Plasmid

#71071

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Su(var)205 (Su(var)205 Fly)

Use

Gateway entry vector

Available Since

Nov. 25, 2015

Availability

Academic Institutions and Nonprofits only
KHBD00295

Plasmid

#34421

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG12052 (lola Fly)

Use

Gateway donor vector

Available Since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00321

Plasmid

#34446

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG8676 (Hr39 Fly)

Use

Gateway donor vector

Available Since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only