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We narrowed to 10,047 results for: pET

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Showing: 3821 - 3840 of 10047 results

  1. pET28-H12TEV-hEIF5B-1~1220(FL)

    Plasmid
    #208636
    Purpose
    for E. coli expression of human eIF5B (ENST00000289371)
    Depositor
    Quanfu Ma
    Insert
    human eIF5B (ENST00000289371) (EIF5B Human)
    Tags
    6xhis
    Expression
    Bacterial
    Mutation
    Contain SNP rs1376443, protein sequence not chang…
    Promoter
    T7
    Available since
    May 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    208636-plasmid-map-sequence-id-433645

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  3. pETM11-6xHis-TEV-hORF1p_R261A

    Plasmid
    #202586
    Purpose
    Allows for IPTG-inducible expression of the human L1RP ORF1 protein with the R261A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification with a minimal scar
    Depositor
    Liam Holt
    Publication
    Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75
    Insert
    ORF1p
    Tags
    6x His and TEV protease cleavage site
    Expression
    Bacterial
    Mutation
    Changed ORF1p Arginine 261 to Alanine
    Available since
    Nov. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    202586-plasmid-map-sequence-id-415635

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  4. pETM11-6xHis-TEV-hORF1p_K3AK4A

    Plasmid
    #202585
    Purpose
    Allows for IPTG-inducible expression of the human L1RP ORF1 protein with the K3A and K4A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification with a minimal scar
    Depositor
    Liam Holt
    Publication
    Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75
    Insert
    ORF1p
    Tags
    6x His and TEV protease cleavage site
    Expression
    Bacterial
    Mutation
    Changed ORF1p Lysine 3 to Alanine, changed ORF1p …
    Available since
    Nov. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    202585-plasmid-map-sequence-id-414431

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  5. pETDuet-1-6xHis-TEV-Nsp8

    Plasmid
    #201022
    Purpose
    Bacterial expression of codon optimized SARS-CoV-2 nsp8 tagged with an N-terminus His-tag followed by a TEV protease cleavage site.
    Depositor
    Sharon Rozovsky
    Publication
    Ghelichkhani et al J Mol Biol. 2023 Apr 15;435(8):168008. d
    Insert
    non-structural protein 8 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Mutation
    Gene insert is codon optimized for Ecoli.
    Promoter
    T7/LacO
    Available since
    May 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    201022-plasmid-map-sequence-id-394460

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  6. pcDNA3-PKAsub-YPet-NES

    Plasmid
    #138203
    Purpose
    Encodes C-terminal (substrate) fragment of FRET-based bimolecular PKA activity reporter (BimAKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES.
    Depositor
    Jin Zhang
    Publication
    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PKAsub-YPet-NES
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    138203-plasmid-map-sequence-id-307404

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  9. 3294_pETcon-SARS2-RBD_Omicron-BA1

    Plasmid
    #184408
    Purpose
    yeast surface display of the SARS-CoV-2 Omicron BA.1 variant RBD
    Depositor
    Jesse Bloom
    Publication
    Starr et al Science. 2022 Jun 28:eabo7896. doi: 10.1
    Insert
    SARS-CoV-2 Omicron BA.1 Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)
    Tags
    HA and c-Myc
    Expression
    Yeast
    Mutation
    G339D+S371L+S373P+S375F+K417N+N440K+G446S+S477N+T…
    Available since
    May 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    184408-plasmid-map-sequence-id-362479

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  13. pETM-11-6His-DmBelle_1-694_B

    Plasmid
    #145951
    Purpose
    Bacterial Expression of DmBelle_1-694
    Depositor
    Elisa Izaurralde
    Insert
    DmBelle_1-694 (bel Fly)
    Expression
    Bacterial
    Mutation
    del 155-157, T530A and I685V
    Available since
    Feb. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    145951-plasmid-map-sequence-id-517522

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  15. pcDNA3-PKCsub(T/A)-YPet-NES

    Plasmid
    #138208
    Purpose
    Encodes negative-control C-terminal (substrate) fragment of FRET-based bimolecular PKC activity reporter (bimCKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES
    Depositor
    Jin Zhang
    Publication
    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PKCsub(T/A)-YPet-NES
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    Target Thr residue in substrate domain mutated to…
    Promoter
    CMV
    Available since
    Sept. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    138208-plasmid-map-sequence-id-307411

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  17. pcDNA3-AMPKsub-YPet-Kras

    Plasmid
    #84638
    Purpose
    Encodes C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); plasma membrane targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES
    Depositor
    Jin Zhang
    Publication
    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AMPKsub-YPet-Kras
    Tags
    6xHis, C-terminal targeting sequence from Kras, T…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    84638-plasmid-map-sequence-id-176882

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Showing: 3821 - 3840 of 10047 results