164,722 results

1. pADH110

   Plasmid

   #90982

   Purpose

   NAT-marked pSNR52 promoter fragment for use in gRNA expression cassette stitching PCR; for use with pADH119, 139, or 147 to generate target-specific gRNA expression cassette.

   Depositor

   Aaron Hernday

   Article

   Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

   Inserts

   NAT 2 of 2
   pSNR52
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   June 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. TFORF3167

   Plasmid

   #144643

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ZNF512B (ZNF512B Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. CBIG-RORb

   Plasmid

   #48709

   Purpose

   bicistronic expression of RORb and EGFP

   Depositor

   Jeffrey Macklis

   Insert

   RORb (Rorb Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/beta-actin

   Available Since

   Oct. 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLC242-GFP-metap2

   Plasmid

   #100518

   Purpose

   Expresses GFP tagged metap2 in mammalian cells to make lentivirus

   Depositor

   David Sabatini

   Article

   Gu et al Science. 2017 Nov 10;358(6364):813-818.

   Insert

   Metap2 (METAP2 Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. Fusion of Zika NS2B-NS3 proteins

   Plasmid

   #204815

   Purpose

   Bacterial expression of a fusion of Zika NS2B-NS3 proteins

   Depositor

   Frank von Delft, ASAP Discovery

   Insert

   Zika NS2B-NS3 fusion (orf1ab Zika)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 25, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pADH100

   Plasmid

   #90980

   Purpose

   NAT-marked "empty" gRNA expression construct; part 2 of 2 of C.alb HIS1-FLP CRISPR system. Use with pADH99 CAS9 expression construct.

   Depositor

   Aaron Hernday

   Article

   Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

   Insert

   NAT 2 of 2, pSNR52, empty gRNA, gRNA conserved, FRT, DS_HIS1
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   June 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. EK0393 SFFV-mTagBFP2-SGc(EGFP.s2)cSG-mCherry (FLP-IN)

   Plasmid

   #191159

   Purpose

   Expression of a sensor for EGFP CDS in mammalian cells; mTagBFP2 marker, mCherry output

   Depositor

   Xiaojing Gao

   Article

   Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

   Insert

   mTagBFP:EGFP.s2:mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   SFFV

   Available Since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. Mito-V5-BS2 (Mitochondrial matrix)

   Plasmid

   #220344

   Purpose

   Mammalian expression vector BS2 in mitocondria

   Depositor

   Bryan Dickinson

   Article

   Pani et al Nat Chem. 2024 Apr 9. doi: 10.1038/s4155

   Insert

   BS2
   

   Use

   Tags

   MTS-matrix and V5 tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 20, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. V5-BS2-SENP2 (Nuclear Pore)

   Plasmid

   #220345

   Purpose

   Mammalian expression vector BS2 in nuclear pore

   Depositor

   Bryan Dickinson

   Article

   Pani et al Nat Chem. 2024 Apr 9. doi: 10.1038/s4155

   Insert

   BS2
   

   Use

   Tags

   SENP2 (nuclear pore localized protein) and V5 tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 20, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pTNT-pT7-tetR-mCherry

    Plasmid

    #140870

    Purpose

    T7 promoter driven synthesis of TetR-mCherry fusion protein

    Depositor

    Neal Devaraj

    Article

    Niederholtmeyer et al Nat Commun. 2018 Nov 28;9(1):5027. doi:

    Insert

    TetR-mCherry expressed with T7 promoter
    

    Use

    Synthetic Biology

    Tags

    mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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