We narrowed to 6,467 results for: poly

1. TBPL1 KO 2

   Plasmid

   #200211

   Purpose

   gRNA for TBPL1 KO

   Depositor

   Sheila Teves

   Article

   Kwan et al Elife. 2023 Mar 30;12:e83810. doi: 10.75

   Insert

   TBP1 KO 2 (Tbpl1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   None

   Promoter

   U6

   Available since

   May 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDGO186N-KS1

   Plasmid

   #174301

   Purpose

   Replicating plasmid with sgRNA cassette containing a killing spacer #1 targeting the wild type polC gene.

   Depositor

   Lee Lynd

   Article

   Lanahan et al Appl Environ Microbiol. 2022 Jan 11;88(1

   Insert

   spacer expression cassette
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDGO186N-KS2

   Plasmid

   #174302

   Purpose

   Replicating plasmid with sgRNA cassette containing a killing spacer #2 targeting the wild type polC gene.

   Depositor

   Lee Lynd

   Article

   Lanahan et al Appl Environ Microbiol. 2022 Jan 11;88(1

   Insert

   spacer expression cassette
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. H1.224.luc

   Plasmid

   #193704

   Purpose

   Expresses a truncated PolII H1 promoter (H1.244)

   Depositor

   Elena Herrera-Carrillo

   Article

   Gao et al J Biol Chem. 2020 Nov 5. pii: S0021-9258

   Insert

   Truncated PolIII H1 promoter of 224 nucleotides (H1.224) + N41 fragment
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. H1.224.M.luc

   Plasmid

   #193705

   Purpose

   Expresses a truncated PolIII H1 promoter of 224 nucleotides (H1.224) with the TATA box mutated

   Depositor

   Elena Herrera-Carrillo

   Article

   Gao et al J Biol Chem. 2020 Nov 5. pii: S0021-9258

   Insert

   Truncated PolIII H1 promoter (H1.224) with the TATA box mutated
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pOP004

   Plasmid

   #185985

   Purpose

   Encodes a 0.8 kb bacterial transcription unit with Broccoli-tRNA fluorogenic aptamer shortly upstream of the intrinsic terminator

   Depositor

   Georgi Belogurov

   Article

   Huang et al Methods Enzymol. 2022;675:207-233. doi:

   Insert

   Bacterial transcription unit encoding Broccoli-tRNA fluorogenic aptamer
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDGO186N-KS3

   Plasmid

   #174303

   Purpose

   Replicating plasmid with sgRNA cassette containing a killing spacer #3 targeting the wild type polC gene.

   Depositor

   Lee Lynd

   Article

   Lanahan et al Appl Environ Microbiol. 2022 Jan 11;88(1

   Insert

   spacer expression cassette
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pOP005

   Plasmid

   #186030

   Purpose

   Encodes a 0.8 kb bacterial transcription unit with Broccoli-tRNA fluorogenic aptamer shortly downstream of the promoter

   Depositor

   Georgi Belogurov

   Article

   Huang et al Methods Enzymol. 2022;675:207-233. doi:

   Insert

   Bacterial transcription unit encoding Broccoli-tRNA fluorogenic aptamer
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. KBB221

   Plasmid

   #185071

   Purpose

   SST::ACT2 "SA236"

   Depositor

   Ken Belanger

   Article

   Belanger et al Planta. 2003 Oct;217(6):931-50. doi: 10.

   Insert

   ACT2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   ACT2-SA236

   Promoter

   Available since

   Aug. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. KBB215

    Plasmid

    #185068

    Purpose

    SST::WAK2 EcoRI/ScaI fragment (secretion confirmed)

    Depositor

    Ken Belanger

    Article

    Belanger et al Planta. 2003 Oct;217(6):931-50. doi: 10.

    Insert

    WAK2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. CDS_Cas9-NLS

    Plasmid

    #136121

    Purpose

    Puchta Arabidopsis codon-optimised Cas9 for CRISPR

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    CDS_AtcoCas9-NLS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. CTAG_eGFP-GHtag-HDEL

    Plasmid

    #136096

    Purpose

    FP with ER retention signal for C-term fusion with CDS12 with ER targeting signal peptide. Contains Gly-His tag

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    CTAG_eGFP(noATG,noStop)-3xGly5xHys-HDEL
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLyGo-Bs-4

    Plasmid

    #163142

    Purpose

    pLyGo cloning vector for a sequence of interest (LPMO) in B. subtilis. Vector encoding the BatLPMO10 signal peptide and the LyGo cassette (SapI-ccdB-SapI)

    Depositor

    Morten Norholm

    Article

    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PamyL-PamyQ-PcryIIIA-cryIIIAstab49

    Available since

    July 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLyGo-Ec-4

    Plasmid

    #163130

    Purpose

    pLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding PhoA signal peptide and the LyGo cassette (SapI-ccdB-SapI)

    Depositor

    Morten Norholm

    Article

    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLyGo-Ec-1

    Plasmid

    #163127

    Purpose

    pLyGo cloning vector for cytoplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding Ubiquitin with an internal His10-tag and the LyGo cassette (SapI-ccdB-SapI)

    Depositor

    Morten Norholm

    Article

    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Ubiquitin

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLyGo-Bs-1

    Plasmid

    #163137

    Purpose

    pLyGo cloning vector for a sequence of interest (LPMO) in Bacillus subtilis. Vector encoding the AmyL signal peptide and the LyGo casette (SapI-ccdB-SapI)

    Depositor

    Morten Norholm

    Article

    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PamyL-PamyQ-PcryIIIA-cryIIIAstab49

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLyGo-Bs-3

    Plasmid

    #163141

    Purpose

    pLyGo cloning vector for a sequence of interest (LPMO) in B. subtilis. Vector encoding the AprE signal peptide and the LyGo cassette (SapI-ccdB-SapI)

    Depositor

    Morten Norholm

    Article

    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PamyL-PamyQ-PcryIIIA-cryIIIAstab49

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLyGo-Bs-2

    Plasmid

    #163140

    Purpose

    pLyGo cloning vector for a sequence of interest (LPMO) in Bacillus subtilis. Vector encoding the AmyQ signal peptide and the LyGo cassette (SapI-ccdB-SapI)

    Depositor

    Morten Norholm

    Article

    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PamyL-PamyQ-PcryIIIA-cryIIIAstab49

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLyGo-Ec-3

    Plasmid

    #163129

    Purpose

    pLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding OmpA signal peptide and the LyGo cassette (SapI-ccdB-SapI)

    Depositor

    Morten Norholm

    Article

    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLyGo-Ec-5

    Plasmid

    #163131

    Purpose

    pLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding DsBA signal peptide and the LyGo cassette (SapI-ccdB-SapI)

    Depositor

    Morten Norholm

    Article

    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge