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Plasmid#1311DepositorInsertHsp104 Y466W (HSP104 Budding Yeast)
UseTagsExpressionYeastMutationChanged Tyr 466 to TrpPromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pGALSc104b Y507W
Plasmid#1312DepositorInsertHsp104 Y507W (HSP104 Budding Yeast)
UseTagsExpressionYeastMutationChanged Tyr 507 to TrpPromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pGALSc104b L553W
Plasmid#1313DepositorInsertHsp104 L553W (HSP104 Budding Yeast)
UseTagsExpressionYeastMutationChanged Leu 553 to TrpPromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pGALSc104b Y819W
Plasmid#1314DepositorInsertHsp104 Y819W (HSP104 Budding Yeast)
UseTagsExpressionYeastMutationChanged Tyr 819 to TrpPromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pGALSc104b L892W
Plasmid#1315DepositorInsertHsp104 L892W (HSP104 Budding Yeast)
UseTagsExpressionYeastMutationChanged Leu 892 to TrpPromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
piHGpd/T22I
Plasmid#1110DepositorInsertHSP82 (HSP82 Budding Yeast)
UseTagsExpressionYeastMutationHSP82 with threonine 22 changed to isoleucine; re…PromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
piHGpd/G81S
Plasmid#1111DepositorInsertHSP82 (HSP82 Budding Yeast)
UseTagsExpressionYeastMutationHSP82 with glycine 81 changed to serine; regulate…PromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
piHGpd/G313S
Plasmid#1114DepositorInsertHSP82 (HSP82 Budding Yeast)
UseTagsExpressionYeastMutationHSP82 with glycine 313 changed to serine; regulat…PromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
piHGpd/E381K
Plasmid#1115DepositorInsertHSP82 (HSP82 Budding Yeast)
UseTagsExpressionYeastMutationHSP82 with glutamic acid 381 changed to lysine; r…PromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
piHGpd/A587T
Plasmid#1116DepositorInsertHSP82 (HSP82 Budding Yeast)
UseTagsExpressionYeastMutationHSP82 with alanine 587 changed to threonine; regu…PromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
piHGpd/A41V
Plasmid#1117DepositorInsertHSP82 (HSP82 Budding Yeast)
UseTagsExpressionYeastMutationHSP82 with alanine 41 changed to valine; regulate…PromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pRS313-prp8-S2197F
Plasmid#89967PurposeS2197F point mutantDepositorInsertPRP8 (PRP8 Budding Yeast)
UseTagsExpressionYeastMutationS2197FPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pRS313-prp8-H2387P
Plasmid#89968PurposeH2387P point mutantDepositorInsertPRP8 (PRP8 Budding Yeast)
UseTagsExpressionYeastMutationH2387PPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pRS313-prp8-R2388G
Plasmid#89969PurposeR2388G point mutantDepositorInsertPRP8 (PRP8 Budding Yeast)
UseTagsExpressionYeastMutationR2388GPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pRS314-prp16-1
Plasmid#89972Purposeprp16-1 allele in TRP plasmidDepositorInsertPRP16 (PRP16 Budding Yeast)
UseTagsExpressionYeastMutationprp16-1 allele (Y386D)PromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pRS314-prp16-101
Plasmid#89973Purposeprp16-101 allele in TRP plasmidDepositorInsertPRP16 (PRP16 Budding Yeast)
UseTagsExpressionYeastMutationprp16-101 allele (G373S)PromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
enz.043-SETD7-Y305F
Plasmid#154045PurposeExpresses SETD7 (Y305F) in E. coliDepositorInsertHistone-lysine N-methyltransferase SETD7 (SETD7 Human)
UseTagsHis, MBPExpressionBacterialMutationchanged tyrosine 305 to phenylalaninePromoterT7Available sinceAvailabilityAcademic Institutions and Nonprofits only -
WT Ecm2
Plasmid#169922PurposeEcm2 +/-300bp of up- and downstream seq. cloned into the NotI and SalI sites of pRS416DepositorInsertEcm2
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pBXNH3CA_MBP
Plasmid#132700Purposeexpresses Avi-tagged MBP in E.coli, to use as positive control in ELISADepositorInsertMaltose binding Protein
UseTagsAvi and HisExpressionBacterialMutationPromoterpBADAvailable sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAC-94N
Plasmid#53281PurposeContains crtI, crtY, and crtI genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and produces beta-carotene in E. coli when complemented with a gene encoding geranylgeranyl diphosphate synthaseDepositorInsertcrtY, crtI, crtB
UseLow copy number bacterial cloning vectorTagsExpressionMutationPromoterendogenous promotersAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSb_init containing the MBP sybody Sb_MBP#1
Plasmid#132699Purposeexpresses the MBP sybody Sb_MBP#1 in E.coli, to use as positive control in ELISADepositorInsertsybody_MBP#1
UseTagsExpressionBacterialMutationPromoterpBADAvailable sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHRdSV40-scFv-GCN4-sfGFP-VP64-GB1-NLS
Plasmid#60904PurposeThe plasmid encodes a antibody that binds to the GCN4 peptide from the SunTag system, and is fused to a transcriptional activation domain VP64DepositorInsertscFv-GCN4
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
His6_MBP_FMRP_P451S
Plasmid#198693PurposeExpression of human FMRP protein in E. coliDepositorInsertFMR1 (FMR1 Human)
UseTagsMBPExpressionBacterialMutationchanged proline 451 to serinePromoterT7Available sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pJBL7045
Plasmid#167216Purposeoverexpression of CueR in E. coli for cell-free copper sensingDepositorInsertCueR
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJBL7062
Plasmid#167231Purposecell-free cascaded copper sensorDepositorInsertpCue-AKSIRV
UseSynthetic BiologyTags6X HisExpressionMutationPromoterAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSETB/mStayGold
Plasmid#212017PurposemStayGold has an n1 adaptor at its N-terminus and is useful for C-terminal tagging. This construct is used also for expression of mStayGold itself.DepositorInsertmStayGold
UseTagsExpressionBacterialMutationPromoterT7Available sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET29a-YS14
Plasmid#66890PurposePolycistronic coexpression of Xenopus laevis histones (H2A, H2B, H3 and H4)DepositorInsertsHis6-Thrombin-Xenopus laevis histones H2A
Xenopus laevis histones H2B
Xenopus laevis histones H3
Xenopus laevis histones H4-Thrombin
UseTagsH2A, H2B, H3, H4, His6 tag, and ThrombinExpressionBacterialMutationPromoterT7Available sinceSept. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPB_mU6_enh-gRNA_Puro-T2A-BFP
Plasmid#235571PurposeEnhanced guide RNA expression & genomic integration. Target gRNA sequences are cloned in via the BstXI and BlpI sites.DepositorInsertsgRNA backbone
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 29, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET15b-RvLEAMshort
Plasmid#218122PurposeExpresses truncated RvLEAM protein under IPTG induction in bacterial host cells.DepositorInsertRvLEAMshort
UseTags6xHISExpressionBacterialMutationTruncated form (58-181aa)PromoterT7Available sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEvolvR-enCas9-PolI5M
Plasmid#113078PurposeExpresses enCas9 fused to PolI5M in E. coli. contains a BsmbI-flanked GFP cassette for sgRNA cloning.DepositorInsertenCas9-PolI5M
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
2C-3XtbGFP-PEST
Plasmid#69072PurposeExpresses 3XturboGFP-NLS-PEST under MERVL_LTRDepositorInsertturboGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
EcSTH
Plasmid#211923PurposeThis plasmid contains human codon optimized sequence of EcSTH which can be used to increase NADH/NAD ratio and decrease NADPH/NADP ratio in mammalian cells.DepositorInsertEcSTH
UseLentiviral and Synthetic BiologyTags3xFLAGExpressionMutationPromoterAvailable sinceApril 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
hSERT-N1-pEYFP
Plasmid#70105Purposemammalian expression of hSERT with C-terminal YFP fusionDepositorInsertSERT (SLC6A4 Human)
UseTagsEYFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
His6-MBP-uTEV3
Plasmid#135464PurposeBacterial expression of uTEV3 (full-length) proteaseDepositorInsertHis6-MBP-uTEV3
UseTagsMBP, His6ExpressionBacterialMutationS219V mutation (improves stability) , I138T/S153N…PromoterT5Available sinceJan. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
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VR124 pETv2 10His-pG-Tn5 (E54K, L372P)
Plasmid#198468PurposeE. coli protein expression plasmid encoding hyperactive Tn5 transposase fused to protein G, in T7 Express lysY/Iq protein expression strain.DepositorInsert10His-pG-Tn5 (E54K, L372P)
UseTags10His-pGExpressionBacterialMutationE54K, L372PPromoterConsensus φ10 T7 PromoterAvailable sinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28-His6-GFP-C-LACE
Plasmid#133913PurposeBacterial expression of His6-GFP-C-LACEDepositorInsertsuperfolder green fluorescent protein
UseTagsLACE tag (GSGPRKVIKMESEE) and TEV-cleavable His6-…ExpressionBacterialMutationPromoterT7Available sinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
lenti-sgRNA-BSD
Plasmid#175583PurposeExpresses sgRNA for CasRx in mammalian cellDepositorInsertsgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceNov. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
MI-Luciferase-IRES-mCherry
Plasmid#75020PurposeEncodes the expression of Luciferase and mCherryDepositorInsertsfirefly Luciferase
mCherry
UseLuciferase and RetroviralTagsExpressionMutationPromoterAvailable sinceJune 1, 2016AvailabilityAcademic Institutions and Nonprofits only