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pFA6a-Pho8∆60-His3MX6

Plasmid

#240971

Purpose

To endogenously tag S. cerevisiae proteins at their C-terminus with Pho8∆60; HIS3 selection marker

Depositor

Fulvio Reggiori

Article

Valdbjorn Kanne et al Autophagy Rep. 2025 May 31;4(1):2511724.

Insert

PHO8∆60 (PHO8 Budding Yeast)

Expression

Bacterial

Available Since

Oct. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pJBL3986

Plasmid

#204132

Purpose

Thermotoga petrophila (T. pe) fluoride riboswitch, wild type

Depositor

Julius Lucks

Article

Hertz et al Nucleic Acids Res. 2024 May 8;52(8):4466

Insert

T. pe fluoride riboswitch sfGFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

Aug. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pJBL811

Plasmid

#204135

Purpose

Low-copy constitutively expressed sfGFP for E. coli

Depositor

Julius Lucks

Article

Hertz et al Nucleic Acids Res. 2024 May 8;52(8):4466

Insert

sfGFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

Aug. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pNC-Y

Plasmid

#238998

Purpose

Node C carrying sgRNA-1 that can be used for the assembly of pEND-11Y or pEND-ZY

Depositor

Javier Santos-Moreno

Article

Nevot et al ACS Synth Biol. 2025 Aug 15;14(8):2878-2

Insert

sgRNA-1 downstream of a weak constitutive promoter and a binding site for sgRNA-2

Use

Synthetic Biology

Expression

Bacterial

Mutation

WT

Available Since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-SV40-puro-EF1a-HA-NLGN1dAChE-bc

Plasmid

#220437

Purpose

Expresses HA-tagged human NLGN1 with K578A/V579A substitutions

Depositor

Paul Blainey

Article

Le et al Cell Rep. 2025 Jun 24;44(7):115889. doi:

Insert

Neuroligin-1 (NLGN1 Human)

Use

Lentiviral

Tags

HA-tag

Expression

Mammalian

Mutation

K578A and V579A substitutions

Promoter

EF1a

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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mAID(KR)–p53 NB(WT)

Plasmid

#232301

Purpose

Hybrid p53 adaptor

Depositor

Masato Kanemaki

Article

Islam et al J Cell Sci. 2025 Jun 9:jcs.263961. doi:

Inserts

Hybrid p53 adaptor
OsTIR1(F74G)

Expression

Mammalian

Available Since

June 16, 2025

Availability

Academic Institutions and Nonprofits only

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mAID(KR)–RAS MB(KR)

Plasmid

#232302

Purpose

KR RAS adaptor

Depositor

Masato Kanemaki

Article

Islam et al J Cell Sci. 2025 Jun 9:jcs.263961. doi:

Inserts

KR RAS monobody
OsTIR1(F74G)

Expression

Mammalian

Available Since

June 16, 2025

Availability

Academic Institutions and Nonprofits only

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mAID(KR)–RAS MB(WT)

Plasmid

#232303

Purpose

Hybrid RAS adaptor

Depositor

Masato Kanemaki

Article

Islam et al J Cell Sci. 2025 Jun 9:jcs.263961. doi:

Inserts

Hybrid RAS adaptor
OsTIR1(F74G)

Expression

Mammalian

Available Since

June 16, 2025

Availability

Academic Institutions and Nonprofits only

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mAID(WT)–GFP NB(WT)

Plasmid

#232297

Purpose

WT GFP nanbody adaptor

Depositor

Masato Kanemaki

Article

Islam et al J Cell Sci. 2025 Jun 9:jcs.263961. doi:

Inserts

WT GFP adaptor
OsTIR1(F74G)

Expression

Mammalian

Available Since

June 16, 2025

Availability

Academic Institutions and Nonprofits only

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mAID(KR)–GFP NB(WT)

Plasmid

#232299

Purpose

Hybrid GFP adaptor

Depositor

Masato Kanemaki

Article

Islam et al J Cell Sci. 2025 Jun 9:jcs.263961. doi:

Inserts

Hybrid GFP adaptor
OsTIR1(F74G)

Expression

Mammalian

Available Since

June 16, 2025

Availability

Academic Institutions and Nonprofits only

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mAID(KR)–p53 NB(KR)

Plasmid

#232300

Purpose

KR p53 adaptor

Depositor

Masato Kanemaki

Article

Islam et al J Cell Sci. 2025 Jun 9:jcs.263961. doi:

Inserts

KR p53 nanobody adaptor
OsTIR1(F74G)

Expression

Mammalian

Available Since

June 16, 2025

Availability

Academic Institutions and Nonprofits only

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pET28a-AviTag-CC-S2

Plasmid

#233243

Purpose

Bacterial expression of Avi-CC-S2

Depositor

Khalid Salaita

Article

Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

Insert

AviTag-CC-S2

Expression

Bacterial

Promoter

T7

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pCBE-dGFP-gRNA

Plasmid

#226862

Purpose

Human gRNA expression vector targeting the Y93H mutation of non-fluorescent EGFP in plasmid pCBE-dGFP; for use with CBEs

Depositor

Alexis Komor

Article

Osgood et al Methods in Enzymology, February 5, 2025

Insert

Sp-gRNA

Expression

Mammalian

Promoter

hU6

Available Since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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apt-CBE-SL3

Plasmid

#219940

Purpose

Mammalian expression of apt-CBE-SL3 protein (MCP_16aa-linker_evoAPOBEC1) with P2A-EGFP marker

Depositor

Alexis Komor

Article

Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

Insert

MCP_16-aa_evo-rA1_2xUGI

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pET29_anti-MERS-Nanobody-VHH-1

Plasmid

#206890

Purpose

Express anti-MERS-Nanobody-VHH-1 in E. coli

Depositor

Magnus Rueping

Article

Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

Insert

anti-mers-nanobody-vhh-1

Tags

6xHis

Expression

Bacterial

Available Since

Jan. 13, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pET29_anti-MERS-Nanobody-VHH-55

Plasmid

#209417

Purpose

Express anti-MERS-Nanobody-VHH-55 in E. coli

Depositor

Magnus Rueping

Article

Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

Insert

anti-mers-nanobody-vhh-55

Tags

6xHis

Expression

Bacterial

Available Since

Jan. 13, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pET29_anti-MERS-Nanobody-VHH-83

Plasmid

#209418

Purpose

express anti-MERS-Nanobody-VHH-83 in E. coli

Depositor

Magnus Rueping

Article

Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

Insert

anti-mers-nanobody-vhh-83

Tags

6xHis

Expression

Bacterial

Available Since

Jan. 13, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pET29_anti-MERS-Nanobody-VHH-84

Plasmid

#209419

Purpose

Express anti-MERS-Nanobody-VHH-84 in E. coli

Depositor

Magnus Rueping

Article

Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

Insert

anti-mers-nanobody-vhh-83

Tags

6xHis

Expression

Bacterial

Available Since

Jan. 13, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5/FRT/TO_eGFP-pScaC-MTS

Plasmid

#228827

Purpose

For the expression of the passenger domain of the Orientia tsutsugamushi protein ScaC C-terminally fused to a mitochondrial targeting sequence in HeLa Flp-In cells

Depositor

Andrew Carter

Article

Manigrasso et al bioRxiv 2024.04.11.589026

Insert

ScaC

Tags

MTS and eGFP

Expression

Mammalian

Mutation

aa 33-223

Available Since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pACEBac1_Strep-Psc-BICD2

Plasmid

#228829

Purpose

Insect cell expression of BICD2

Depositor

Andrew Carter

Article

Manigrasso et al bioRxiv 2024.04.11.589026

Insert

BICD2 (Bicd2 Mouse)

Tags

Strep

Expression

Insect

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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