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msHook2 g1 lentiCRISPRv2-mCherry

Plasmid

#218652

Purpose

Knockout vector for mouse Hook2

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

Hook2 (Hook2 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Aug. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221 msNLRP3 (STOP)

Plasmid

#218638

Purpose

Gateway shuttling vector for cloning N-terminal NLRP3 tags (contains a stop codon--note: bulky N-terminal tags are likely not functional)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

NLRP3 (Nlrp3 Mouse)

Use

Gateway Cloning

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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msHook2 g2 lentiCRISPRv2-mCherry

Plasmid

#218653

Purpose

Knockout vector for mouse Hook2

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

Hook2 (Hook2 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221-kcnk13b

Plasmid

#208997

Purpose

Riboprobe generation

Depositor

GuangJun Zhang

Article

Park et al Dev Dyn. 2024 Jan 25. doi: 10.1002/dvdy.

Insert

kcnk13b (kcnk13b )

Use

Cloning

Promoter

T7

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221-kcnk15

Plasmid

#208998

Purpose

Riboprobe generation

Depositor

GuangJun Zhang

Article

Park et al Dev Dyn. 2024 Jan 25. doi: 10.1002/dvdy.

Insert

kcnk15 (kcnk15 Zebrafish)

Use

Cloning

Promoter

T7

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221-kcnk20

Plasmid

#209002

Purpose

Riboprobe generation

Depositor

GuangJun Zhang

Article

Park et al Dev Dyn. 2024 Jan 25. doi: 10.1002/dvdy.

Insert

kcnk20 (si:ch211-261a10.5 Zebrafish)

Use

Cloning

Promoter

T7

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221-kcnk1b

Plasmid

#208982

Purpose

Riboprobe generation

Depositor

GuangJun Zhang

Article

Park et al Dev Dyn. 2024 Jan 25. doi: 10.1002/dvdy.

Insert

kcnk1b (kcnk1b Zebrafish)

Use

Cloning

Promoter

T7

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR-D-Topo-kcnk12

Plasmid

#208995

Purpose

Riboprobe generation

Depositor

GuangJun Zhang

Article

Park et al Dev Dyn. 2024 Jan 25. doi: 10.1002/dvdy.

Insert

kcnk12 (kcnk12 Zebrafish)

Use

Cloning

Promoter

T7

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pCB40

Plasmid

#205136

Purpose

Integrating expression of SnxA-GFP in Dictyostelium

Depositor

Jason King

Article

Vines et al J Cell Biol. 2023 Sep 4;222(9):e20220907

Insert

SnxA

Use

Dictyostelium expression

Tags

EGFP

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pSLIK BirA* zeo

Plasmid

#192332

Purpose

Lentiviral expression vector for an inducible 3xFLAG-BirA

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

BirA*

Use

Lentiviral

Tags

3xFLAG

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pEN_TT BirA* w ccdB

Plasmid

#192310

Purpose

Gateway entry vector for cloning

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

BirA*

Use

Gateway Cloning

Tags

3xFLAG

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pEN_TT 3xFLAG-Linker

Plasmid

#192305

Purpose

Gateway entry vector for cloning

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

Linker

Use

Gateway Cloning

Tags

3xFLAG

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-NcPAN3_234-656_U

Plasmid

#147680

Purpose

Bacterial Expression of NcPAN3_234-656

Depositor

Elisa Izaurralde

Article

Christie et al Mol Cell. 2013 Aug 8;51(3):360-73. doi:

Insert

NcPAN3_234-656

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pMPRAduo:∆orf

Plasmid

#193740

Purpose

MPRAduo backbone

Depositor

Ryan Tewhey

Article

Mouri et al Cell Genom. 2022 Dec 16;3(1):100234. doi

Type

Empty backbone

Expression

Mammalian

Promoter

none

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pYTK001_P2Aq(2nd)

Plasmid

#183089

Purpose

2A peptide P2A cloned in yeast MoClo entry vector (pYTK001) for use as the second 2A peptide in quad-cistronic construct assembly

Depositor

Zhen Wang

Article

Mukherjee et al Biotechnol Bioeng. 2022 Sep 27. doi: 10.

Insert

P2A

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2094

Plasmid

#162142

Purpose

Complementation plasmid for MlaE E98A

Depositor

Gira Bhabha, Damian Ekiert

Article

Coudray et al Elife. 2020 Nov 25;9. pii: 62518. doi: 1

Insert

MlaFEDCB

Expression

Bacterial

Mutation

MlaF-MlaE(E98A)-(6xHis-MlaD)-MlaC-MlaB

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2095

Plasmid

#162143

Purpose

Complementation plasmid for MlaE K205A

Depositor

Gira Bhabha, Damian Ekiert

Article

Coudray et al Elife. 2020 Nov 25;9. pii: 62518. doi: 1

Insert

MlaFEDCB

Expression

Bacterial

Mutation

MlaF-MlaE(K205A)-(6xHis-MlaD)-MlaC-MlaB

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2097

Plasmid

#162144

Purpose

omplementation plasmid for MlaE D250A

Depositor

Gira Bhabha, Damian Ekiert

Article

Coudray et al Elife. 2020 Nov 25;9. pii: 62518. doi: 1

Insert

MlaFEDCB

Expression

Bacterial

Mutation

MlaF-MlaE(D250A)-(6xHis-MlaD)-MlaC-MlaB

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2057

Plasmid

#162145

Purpose

Expression plasmid for MlaE Tyr81Bpa mutant for in vivo crosslinking

Depositor

Gira Bhabha, Damian Ekiert

Article

Coudray et al Elife. 2020 Nov 25;9. pii: 62518. doi: 1

Insert

MlaFEDCB

Expression

Bacterial

Mutation

MlaF-(6xHis-MlaE, Tyr81Bpa)-MlaD-MlaC-MlaB

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2060

Plasmid

#162146

Purpose

Expression plasmid for MlaE Val77Bpa mutant for in vivo crosslinking

Depositor

Gira Bhabha, Damian Ekiert

Article

Coudray et al Elife. 2020 Nov 25;9. pii: 62518. doi: 1

Insert

MlaFEDCB

Expression

Bacterial

Mutation

MlaF-(6xHis-MlaE, Val77Bpa)-MlaD-MlaC-MlaB

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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