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We narrowed to 4,823 results for: donor

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Showing: 3841 - 3860 of 4823 results

  1. pAAVS1-Puro-CAG-mNG2(1-10)

    Plasmid
    #206042
    Purpose
    Repair template for the integration of a mNG2(1-10) expression cassette into the AAVS1 locus in human cells.
    Depositor
    Alisa Piekny
    Publication
    Husser et al Elife. 2024 Apr 23;12:RP92819. doi: 10.7
    Insert
    AAVS1 homology arms with SA-P2A-Puro marker and CAG-mNG2(1-10) expression cassette
    Use
    CRISPR and Synthetic Biology; Donor template
    Expression
    Mammalian
    Available since
    Oct. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    206042-plasmid-map-sequence-id-411117

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  2. AICSDP-26:ST6GAL1-mEGFP

    Plasmid
    #101786
    Purpose
    Homology arms and linker-mEGFP sequence for C-terminus tagging of human ST6GAL1
    Depositor
    Allen Institute for Cell Science
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ST6GAL1 Homology Arms with linker-mEGFP (ST6GAL1 Human)
    Use
    CRISPR; Donor template
    Tags
    linker-mEGFP
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available since
    Oct. 31, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    1 Article
    101786-plasmid-map-sequence-id-197675

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  3. AICSDP-22:CETN2-mTagRFP-T

    Plasmid
    #101781
    Purpose
    Homology arms and mTagRFPT-linker sequence for N-terminus tagging of human CETN2
    Depositor
    Allen Institute for Cell Science
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CETN2 Homology Arms with mTagRFPT-linker (CETN2 Human)
    Use
    CRISPR; Donor template
    Tags
    mTagRFPT-linker
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available since
    Oct. 31, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    1 Article
    101781-plasmid-map-sequence-id-197673

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  4. AICSDP-69: NPM1-mTagRFP-T

    Plasmid
    #124608
    Purpose
    Homology arms and linker-mTagRFP-T sequence for C-terminus tagging of human NPM1
    Depositor
    Allen Institute for Cell Science
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NPM1 Homology Arms with linker-mTagRFP-T (NPM1 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Available since
    May 13, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    124608-plasmid-map-sequence-id-244304

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  6. pSLS.492

    Plasmid
    #184957
    Purpose
    Test effect of a1/a2 length on editing rpoB using retron recombineering
    Depositor
    Seth Shipman
    Publication
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1 RT and recombineering ncRNA, rpoB T1534C, a1/a2 length: 22
    Expression
    Bacterial
    Mutation
    rpoB donor T1534C, a1/a2 length extended to 22 bp
    Promoter
    T7/lac
    Available since
    Nov. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    184957-plasmid-map-sequence-id-376681

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  7. AAVS1-SAGFP

    Plasmid
    #127100
    Purpose
    SA-GFP substrate (Addgene #41594) flanked by human AAVS1 homology arms. Endogenous AAVS1 promoter drives T2A-hygromycin expression after integration. SA-GFP cassette contains PGK-puromycin.
    Depositor
    Adam Karpf
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SA-GFP reporter
    Expression
    Mammalian
    Available since
    June 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    127100-plasmid-map-sequence-id-247555

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  8. AICSDP-117:POLR2A-mEGFP

    Plasmid
    #164500
    Purpose
    Homology arms and mEGFP-linker sequence sequence for N-terminus tagging of human POLR2A
    Depositor
    Allen Institute for Cell Science
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    POLR2A Homology Arms with mEGFP-linker (POLR2A Human)
    Use
    CRISPR; Donor template
    Tags
    mEGFP-linker
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available since
    Feb. 17, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    164500-plasmid-map-sequence-id-324900

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  9. AICSDP-63: ACTN2-mEGFP

    Plasmid
    #124607
    Purpose
    Homology arms and linker-mEGFP sequence for C-terminus tagging of human ACTN2, via excisable Cas9-excisable CAGGS-mCherry selection cassette
    Depositor
    Allen Institute for Cell Science
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ACTN2 Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (ACTN2 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Available since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    124607-plasmid-map-sequence-id-244336

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  10. C17A

    Plasmid
    #27801
    Depositor
    Steven Vogel
    Publication
    Koushik et al Biophys J. 2006 Dec 15. 91(12):L99-L101.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cerulean-17-Amber
    Use
    Fret donor control
    Tags
    Amber and Cerulean
    Expression
    Mammalian
    Available since
    May 23, 2011
    Availability
    Academic Institutions and Nonprofits only

  11. AICSDP-57: SMC1A-mEGFP

    Plasmid
    #114406
    Purpose
    Homology arms and linker-mEGFP sequence for C-terminus tagging of human SMC1A
    Depositor
    Allen Institute for Cell Science
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SMC1A Homology Arms with linker-mEGFP (SMC1A Human)
    Use
    CRISPR; Donor template
    Tags
    linker-mEGFP
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available since
    Sept. 4, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    2 Articles
    114406-plasmid-map-sequence-id-220833

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  12. AAVS1-PDL1

    Plasmid
    #159280
    Purpose
    Human AAVS1 targeting vector for knockin of a CAGGS promoter-driven human PDL1/CD274 (cloned between AgeI and PacI). Targeted cells will be puromycin resistant.
    Depositor
    Rudolf Jaenisch
    Publication
    Ma et al Cell Rep Med. 2020 Sep 22;1(6):100097. d
    Insert
    Human PDL1/CD274 (CD274 Human)
    Use
    AAV; S1 knockin donor vector
    Expression
    Mammalian
    Promoter
    CAGGS
    Available since
    Sept. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    159280-plasmid-map-sequence-id-312917

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  14. SOX9-T2A-H2B-EGFP repair template

    Plasmid
    #167972
    Purpose
    SOX9 reporter repair template
    Depositor
    Emma Rawlins
    Publication
    Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SRY-Box Transcription Factor 9 (SOX9 Human)
    Use
    CRISPR; Donor template
    Tags
    H2B-EGFP
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available since
    June 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    167972-plasmid-map-sequence-id-331137

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  15. AICSDP-27:SLC25A17-mEGFP

    Plasmid
    #101784
    Purpose
    Homology arms and linker-mEGFP sequence for C-terminus tagging of human SLC25A17
    Depositor
    Allen Institute for Cell Science
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SLC25A17 Homology Arms with linker-mEGFP (SLC25A17 Human)
    Use
    CRISPR; Donor template
    Tags
    linker-mEGFP
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available since
    Oct. 31, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    1 Article
    101784-plasmid-map-sequence-id-197677

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  17. C32A

    Plasmid
    #27802
    Depositor
    Steven Vogel
    Publication
    Koushik et al Biophys J. 2006 Dec 15. 91(12):L99-L101.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cerulean-32-Amber
    Use
    Fret donor control
    Tags
    Amber and Cerulean
    Expression
    Mammalian
    Available since
    May 23, 2011
    Availability
    Academic Institutions and Nonprofits only

  19. pAAV KI Cloning Vector_hSyn mTagBFP2-CAAX2

    Plasmid
    #236245
    Purpose
    Cloning template for knock-in based strategy with U6 promoter, gRNA and donor tag cloning cassettes, and a fluorescent marker for the plasma membrane under hSyn promoter
    Depositor
    Jennifer Lippincott-Schwartz
    Publication
    Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do
    Insert
    mTagBFP2 fused to the plasma membrane targeting sequence CAAX2
    Use
    AAV
    Tags
    mTagBFP2
    Promoter
    U6 and human Synapsin1
    Available since
    May 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    236245-plasmid-map-sequence-id-471239

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Showing: 3841 - 3860 of 4823 results